Cardiac protective role of a novel erythrocyte-derived depressing factor on rats and its Ca^2＋ mechanism

The cardiac protective role of a novel erythro-cyte-derived depressing factor (EDDF) on spontaneous hy-pertensive rats (SHR), calcium overload (CaO) rats and Wistar rats and its mechanism was evaluated. Mean artery pressure (MAP), heart rate (HR) and LVdp/dtmax were measured by physiological recorder. The effect of EDDF on the Ca2+-ATPase activity in myocardial sarcoplasmic reticu-lum (SR) of CaO rats was determined by inorganic phos-phate assay. Calcium transport in myocytes was measured by 45Ca2+ radioactive isotope measurement. The phosphoryla-tion levels of extracellular signal-regulated protein kinases (ERK1/2) in myocardial tissue of SHR and CaO rats were measured by Western blot method. And the ultrastructures of cardiac muscle cells were observed with the transmission electron microscope. The results indicated that EDDF could significantly decrease MAP, HR and LVdp/dtmax in a dose dependent manner (P < 0.05). It seems that the mechanism might relate with activating the Ca2+-APTase, enhancing the uptake and release of Ca2+ from SR (P < 0.05), decreasing the phosphorylation levels of ERK1/2 of myocytes (P < 0.01) and lightening the ultrastructural lesion of cardiac muscle cells. In CaO rats, the Ca2+-ATPase activity decreased clearly com-pared to control (64.99 7.16 vs 94.48 7.68 nmol·min-1 ·mg-1 protein, P < 0.01), while EDDF (100 mg/mL) could significantly increase the activity (87.93 ?9.54 vs 64.99 ?7.16, P < 0.05, n = 7). Both uptake and release rate of Ca2+ (祄ol 45Ca2+/g protein/min) from myocardial SR of CaO rats re-markably decreased compared to control (32.40 ?2.70 and 15.46 ?1.49 vs 61.09 ?10.89 and 25.47 ?4.29, P < 0.05); EDDF (100 mg/mL) could significantly stimulate their activi-ties (50.48 6.76 and 21.76 2.75 vs 32.40 2.70 and 15.46 1.49, P < 0.05). EDDF could evidently down-regulate the phosphorylation of ERK1/2 in myocardial tissue from SHR and CaO rats (P < 0.01), lighten the ultrastructural lesion of cardiac muscle cells of SHR as well. It is concluded that EDDF seems to play protective roles on both structure and function of heart, which closely related with amelioration of Ca2+ transport and inhibition of Ca2+-MAP kinase pathway.     

沥青路面防止反射裂缝措施的试验路研究

针对半刚性基层沥青路面的反射裂缝问题,采取基层预锯缝和铺设土工布和APP改性沥青油毡的方法进行试验研究,为更加深入地开展减缓甚至在某种程度上防止部分基层裂缝的措施和方法,积累资料和经验.     

浅谈教学中如何培养学生的创造性思维

结合笔者多年教学经验,阐述了在实际教学过程中培养学生创新性思维的重要性,并提出培养学生创新性思维的方法。     

公路环境景观设计

对公路建设与环境的关系、公路景观设计的原则和主要的景观设计内容及方式进行了介绍和论述。     

沥青路面防止反射裂缝措施的试验路研究

针对半刚性基层沥青路面的反射裂缝问题,采取基层预锯缝和铺设土工布和APP改性沥青油毡的方法进行试验研究,为更加深入地开展减缓甚至在某种程度上防止部分基层裂缝的措施和方法,积累资料和经验。     

浅谈教学中如何培养学生的创造性思维

结合笔者多年教学经验,阐述了在实际教学过程中培养学生创新性思维的重要性,并提出培养学生创新性思维的方法。     

用进化-模拟退火算法求解Lambert方程

Lambert方程在轨道拦截和初始轨道确定起着重要作用。求解Lambert方程的传统算法主要有Newton迭代方法和超几何级数展开方法等,但这些算法都有一定的局限性(如有可能出现迭代收敛过慢,级数展开收敛性问题)。采用进化-模拟退火算法(EA-SA)算法求解Lambert方程,其中进化算法具有全局搜索能力,而模拟退火具有局部锁搜索能力。该方法克服了某些情况下梯度下降法有时收敛过慢和超几何级数不收敛的缺点,并具有通用性,便于操作和理解。通过仿真计算对比表明,EA-SA具有普适性,而且精度优于其它两种算法。     

Musielak-Orlicz-Sobolev空间关于Luxemburg范数的端点

本文着重讨论Musielak-Orlicz-Sobolev空间端点的性质,得到Musielak-Orlicz-Sobolev空间关于Luxemburg范数端点的充分条件.     

Ag纳米线过热的热力学模型

对Ag纳米线的熔化和过热作了研究,提出了一种经典热力学模型对Ag纳米线的过热机制作了阐述;当考虑到低能Ag/Ni界面的作用时,推导了过热的定量表达式,说明了过热与纳米线的半径和界面能有密切关系.     

袋装水泥土材料的力学性能试验研究

袋装水泥土是一种新型的砌筑材料.针对一2160 mm×1000 mm×2250 mm挡土墙的袋装水泥土试块进行了一系列的实验研究,对挡土墙中的袋装水泥土试块在不同龄期、不同堆积荷载、浸泡与不浸泡的条件下的无侧限抗压强度进行了对比分析;讨论了模型袋、堆积荷载、龄期、浸泡对袋装水泥土的无侧限抗压强度的影响,以及同条件下不同龄期和不同堆积荷载下的袋装水泥土的强度关系式,以及相同配比、相同龄期的袋装水泥土在浸泡后与浸泡前强度比值,为其用于实际工程提供了实验依据.