井条件下的几何因子理论

本文论述了有井和无井时多层介质的测井响应与两个无限均匀介质的界面响应之间的关系。将分层均匀地层的测井响应化简成关于各层物理特征的代数方程,并定义了各种测井方法的几何因子和井条件下的几何因子,提出了该几何因子的计算方法及实际应用时的成立条件,并给出了验证实例。     

Formation of carthamin by a partially purified enzyme from safflower seedlings

Summary An enzyme responsible for synthesizing carthamin from precarthamin was partially purified and the catalytic properties were investigated.Acknowledgments. The authors wish to thank Prof. A. Komamine, University of Tokyo, for reading this paper. We are indebted to Prof. A. Saito, Tokai University, for performing emission spectrochemical analysis of the enzyme protein used in this study.     

Peripheral neuropathies, hepatocellular carcinomas and islet cell adenomas in transgenic mice

The ability to introduce foreign DNA into the genome of mice offers unique opportunities to produce new models of disease process. Recent experiments have shown that integration and expression of simian virus 40 (SV40) T antigen genes and the murine mammary tumour virus (MMTV)-myc genes in transgenic mice can lead to the development of neoplasia in a remarkably tissue-specific manner. In the case of SV40-bearing mice, tumours consistently develop in the choroid plexus. In the accompanying paper, we show that the 72-base pair (bp) enhancer in the SV40 genome is instrumental in directing tumorigenesis to the choroid plexus. However, when the enhancer is deleted from a construction also containing the metallothionein-human growth hormone fusion gene (SV delta e-MGH), an entirely new pattern of pathology results. The present report focuses on transgenic mice carrying this construct; they develop demyelinating peripheral neuropathies, hepatocellular carcinomas and islet cell adenomas.     

棉纤维素/N-甲基吗啉-N-氧化物—水合物(NMMO·H_2O)溶液的流变性能

利用Instron3211型毛细管流变仪和Instron3325型流变力学谱仪研究了棉纤维素/N-甲基吗啉-N-氧化物一水合物(NMMO·H_2O)溶液流变性能,讨论了剪切速率、温度和溶液中的纤维素的质量分数对棉纤维素/NMMO·H_2O溶液流变性能的影响,计算了不同质量分数溶液的粘流活化能。     

Regional distribution of thiamin pyrophosphokinase in rat brain

Summary The highest specific activity of thiamin pyrophosphokinase was found in the cerebellum, and lower activity in cerebral cortex and midbrain. The regional difference in the enzyme activity was similar to that in thiamin content and the influx rate in rat brain, suggesting that the enzyme is involved in the thiamin transport.     

涡轮流量计数学模型与优化设计

本文应用流体力学边界层理论,对涡轮流量计涡轮叶片受到的流体粘性阻力矩作了较详尽的理论分析,得到了粘性阻力矩的计算公式。公式表明,在层流流动时,粘性阻力矩与流量的3/2次方成正比;在湍流流动时与流量成13/7次方关系。从而导出了全新的涡轮流量计的数学模型。根据这一数学模型计算的涡轮流量计的特性曲线与实验结果甚为吻合。文中还提出了关于涡轮流量计临界雷诺数Re_(c1)的新概念。认为可以用Re_(c1)的值作为判别涡轮流量计特性曲线优劣的准则数,对仪表结构参数进行优化设计,以扩大仪表的量程比。     

用边界单元法求解三维弹塑性问题的格式

基于初应力法弹塑性理论,给出了求解三维弹塑性问题边界单元法的一种有效的格式,它适用于较复杂的本构关系,在迭代格式和处理内点奇异积分方面所采用的方法是有效的,能保证精度并加快求解速度。     

Unusual helical packing in crystals of DNA bearing a mutation hot spot

The target sequence of the restriction enzyme NarI (GGCGCC) is a hot spot for the -2 frameshift mutagenesis (GGCGCC----GGCC) induced by the chemical carcinogens such as N-2-acetyl-aminofluorene. Of the guanine residues, all of which show equal reactivity towards the carcinogen, only binding to the 3'-most proximal guanine within the NarI site is able to trigger the frameshift event. We selected the non-palindromic dodecamer d(ACCGGCGCCACA), whose sequence corresponds to the most mutagenic NarI site in pBR322 DNA; for X-ray structure analysis. Its molecular structure determined at 2.8 A resolution reveals significant deviations from the structure of canonical B-form DNA, with partial opening of three G-C base pairs, high propeller twist values and sequence-dependent three-centred hydrogen bonds. This crystal structure shows a novel kind of packing in which helices are locked together by groove-backbone interactions. The partial opening of G-C base pairs is induced by interactions of phosphate anionic oxygen atoms with the amino group of cytosine bases. This provides a model for close approach of DNA molecules during biological processes, such as recombination.     

Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intermolecular process

Subtilisin E, an alkaline serine protease consisting of a single polypeptide chain of 275 amino acids is produced from a pre-pro-protein. The pre-sequence functions as the signal peptide for protein secretion across the membrane. Deletion of the pro-sequence yields mature but inactive subtilisin: the 77-amino acid pro-sequence must precede the mature subtilisin to guide the latter into an active conformation. Pro-subtilisin denatured in 6 M guanidine-HCl can be self-processed to the active enzyme intramolecularly, with concomitant cleavage of the pro-sequence, when dialysed against renaturing buffer. We have constructed an active-centre mutant of pro-subtilisin (Asp 32----Asn) which is not processed to active enzyme, unlike the wild-type pro-subtilisin, because intramolecular processing is prevented. Here we report an intermolecular pathway for the refolding of the inactive mature protein to an active enzyme in vitro with the aid of exogenously added pro-sequence. We establish conditions under which the mature inactive form, as well as acid-denatured subtilisins Carlsberg and BPN', can be renatured by the mutant pro-subtilisin.     

钻孔灌注桩常见施工质量问题及防治措施

文章通过介绍水下灌注桩施工中常见事故出现的原因,总结施工中应注意的各种预防措施及事后补救办法。