Expression, purification of a synthetic fuse-protein-TSF from PF4 and TSP1 fragments and its effect on angiogenesis and tumor growth

Sequences encoding PF4 (58–70) and TSP1 (429–459) were linked to yield a single gene TSF which encodes the fuse-protein of TSF. The gene was cloned into a pGEX-2T expression vector to generate a protein GST-TSF, which was strongly expressed inE. coli. The purified GST-TSF was degraded with thrombin to generate the protein TSF. With the methods of MTT and wound repair assay, the effects of TSF on the proliferation and migration of EC were detected, respectively. The results showed that TSF significantly suppressed BAEC proliferation and migration in a dose-dependent manner. The fuse protein GST-TSF, and the peptides PF4 (58–70) and TSP1 (429–459) also inhibited BAEC proliferation and migration, respectively, but their inhibition rates were not as high as TSF. Using the CAM assay, it was shown that TSF, GST-TSF, PF4 (58–70) and TSP1 (429–459) inhibited angiogenesis in chick CAM potentially, the effect of TSF was the highest.In vivo, the growth of Lewis lung carcinoma was potently inhibited by TSF treatment, and the inhibition rate was 68.75% at a dose of 1.00 μmol/kg · d. These findings suggest that the design on TSF gene was successful, and TSF with its anti-angiogenic and anti-tumor activity, should be a useful source of the inhibitor.     

Microtextural characterization of as-cast and hot press-deformed TiN whiskers in TiNw-AINp/Al composite

The local distribution of preferred orientation and misorientation in TiN whiskers in TiNw-AINp/A1 composite system is investigated using backscattering electron diffraction combined with a scanning electron microscope. These preliminary results demonstrate the utility of this new automated technique for characterizing local textures in composite materials using backscattering electron diffraction. The composite investigated comprises TiN whiskers in a polycrystalline aluminum matrix. The local textures of samples before and after deformation were measured. The misorientation distributions show a breakdown of the monocrystal of TiN whisker in the deformed composite material relative to the starting material and an increase in small angle misorientations.     

介质阻挡放电照相技术的应用

在研究介质阻挡放电照相机理和质量的基础上,采用高压纳秒脉冲介质阻挡放电技术,以硬币、植物叶片以及人体手指为样品,对五种应用实例进行了实验尝试和讨论。结果表明,用该方法在拍摄单一色彩的非平面图案时,所得的图像比用普通光学摄影获得的图像更为完整、清晰、且对比度高。该技术可应用于非平面样品图案图像的提取和复印、植物新鲜程度的鉴别、人体指纹的获取以及特殊摄影等领域。     

彩色等离子体显示器智能接口电路的研究

提出了可自动识别信号制试和格式的彩色等离子体显示（PDP）接口电路和实现方法,该电路可智能识别并适应常见不同分辨率的VGA信号格式和NTSC、PAL等两种电视制式,其音频处理部分支持最新的丽音标准,带有杜比Pro Logic处理功能。研究和实验结果发现,本接口电路应用于54cm和107cm彩色PDP的图像显示效果均很好,完全达到了实用化的程度。     

考虑横向剪切效应的竹材层合板弯曲变形

用层梁经典理论,一阶剪切变形理论以及有限单元法分别计算了竹材层合板梁的弯曲变形,为验证理论分析与数值计算结果是否合理,对实际结构变形进行了测定。结果显示,竹材层合板梁在跨高比较小时具有十分显的的横向剪切交 ,一阶剪切变形理论与有限单元法均能足够精确地反映含有剪切效应的梁的弯曲变形,横向剪切效应很小时,经典理论的解与实际情况的符合程序优于一阶剪切变形理论。     

Li1+2x+yAlxEuyTi2-x-ySixP3-xO12系统锂快离子导体

Li1+2x +yAlxEuyTi2 -x -ySixP3 -xO12 锂快离子导体可用精选的天然高岭石Al4 [Si4 O10 ](OH) 8为原料 ,与Li2 CO3,TiO2 ,NH4 H2 PO4 ,Eu2 O3 高温 (80 0～ 10 0 0℃ )固相反应约 2 0h而制得 .在 y =0 .5 ,x≤ 0 .3 ;x =0 .2 ,y≤ 0 .3的原始组成范围内 ,可以形成一个空间群为R3C的固熔体相 .它们具有较好的离子电导率和较小的离子导电活化能 .当x =0 .2 ,y =0 .1,t=40 0℃时 ,其电导率达 9.98mS·cm-1,其活化能为 34.0kJ.mol-1.     

邻硝基对甲基苯酚的相转移催化合成

研究了用季nin盐作相转移催化剂催化合成邻硝基对甲基苯酚,在18％的硝酸、1．0g催化剂、反应温度20℃～25℃、反应45min的条件下,获得70％以上的产率。此法具有产率高、易操作、反应条件温和、催化剂能重复使用的优点。     

弱光Hartmann-Shack波前传感器的阈值选取

研究以像增强CCD为光电探测器的弱光Hartmann-Shack(H-S)波前传感器在质心同探测过程中阈值选取对质心探测精度的影响,首先给出了阈值选取对质心探测系统误差和随机误差的影响,一义质心测量精度的评价指标,并根据此指标指出阈值选取的最佳范围为－3σr≤Tm≤-σr,并以实验验证了理论分析结果。     

煤的可溶化技术与煤的化学族组成

综述了煤可溶化技术研究的现状,讨论了影响煤可溶化的主要因素以及几种强化方法,指出了今后应作深入研究的几个方面,并提出了利用煤的可深化技术进行煤的化学族组成研究的设想。     

供应链管理中的生产批量与数：折扣定价联合决策模型

理论研究与企业实践表明,激烈的市场竞争要求企业必须抛弃传统基于劳 动分工的职能管理思想,而采用以流程为基础的集成化管理的思想．为此,传统的生 产、营销等职能部门的决策应该得到有效的协调．本文在供应链背景下提出了一种生 产批量与定价的联合决策模型．在分析了最优联合决策的性质后,开发了求解模型的 算法,以有效地辅助供应链协调管理．,It is shown that the process-based management philosophy should be used to replace the la bor-division-based management philosophy so that the company can survive in the intensive competitive market. For this reason, the traditional department of production, marketing and finance should be coor dinated. In this paper, a joint decision model is proposed for the management of production decision and the marketing decision in a supply chain. In the model, the marketing variable is the quantity discount pricing policy and the production variable is the lot-sizing policy, which were studied separately in the past researches. After the properties of the solution to the model are studied, an effective algorithm is de veloped for supporting the supply chain management.