铂(II)与胺类配合物的合成、晶体结构和抗肿瘤活性研究

研究铂(II)与三种胺类配合物的合成、晶体结构和体外抗肿瘤活性,化合物1的X-射线单晶结构分析表明:这是一个很少见的具有四方锥几何构型的铂(II)配合物,顺式平面四方形配位的铂的轴向上有第5个配位原子。其抗肿瘤活性实验显示3个配合物对几种研究的癌细胞都有较强的抑制作用。     

采用大规模专用OSDC芯片的数字视频混合器研究

讨论了基于大规模OSDC(0n Screen Display Control)集成电路的数字视频混合器(Video Digital Mixer)的设计原理．数字视频混合器广泛应用于高速公路、治安卡口、十字路口等交通管理领域，对于自动化和智能管理有着重要的作用．现在普遍使用的数字视频混合器存在功能单一、无法显示彩色字符、采用低速的串行通信接口、字库容量小、没有时间日期显示、字符显示和视频信号无法同步等问题．所述的数字视频混合器实现了在输入的视频信号上叠加可自定义的字符或图符，通过PC机的PCI接口实现对字符显示的位置、字符的大小、字符的颜色等的控制．内置的时间、日期控制器可以协同控制单元实现平滑、无闪烁的时间和日期显示．采用带看门狗功能中央处理期能够保证系统的高度稳定，独特的双端口总线实现了对闪存的单向写入和双向读出。对所用到的主要集成芯片的功能和特点进行了说明．将采用大规模集成电路设计的数字视频混合器和以前使用中小规模集成电路设计的数字视频混合器进行了对比。得到了非常显的技术进步．     

边界单元法三维应力分析计算程序

以三维弹性力学问题的基本解 (Kelvin解 )为基础开发了边界单元法三维弹性应力分析计算程序 ,并对其进行了验证。结果表明 ,该程序可用来求解三维弹性应力问题 ,尤其适用于三维应力集中问题。程序中采用动态分配内存 ,自动选点积分 ,并利用分块解法求解方程组 ,有效地节省了计算机资源 ,扩大了求解问题的规模。     

Bi含量对SrBi4Ti4O15铁电材料性能的影响

~~Bi含量对SrBi_4Ti_4O_(15)铁电材料性能的影响@朱骏$扬州大学物理科学与技术学院!江苏扬州,225002 @毛翔宇$扬州大学物理科学与技术学院!江苏扬州,225002 @卢网平$扬州大学物理科学与技术学院!江苏扬州,225002 @惠荣$扬州大学物理科学与技术学院!江苏扬州,225002 @陆文峰$扬州大学物理科学与技术学院!江苏扬州,225002 @陈小兵$扬州大学物理科学与技术学院!江苏扬州,225002~~~~~~~~     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

Evolution of the South China Sea and monsoon history revealed in deep-sea records

As the third summary report of ODP Leg 184 to the South China Sea (SCS), this paper discusses the evolution of the East Asian monsoon and the SCS basin. A multi-proxy approach, involving geochemistry, micropale-ontology, pollen and other analyses, was adopted for reconstructing the evolutionary history of the East Asian monsoon, which was characterized by a series of paleo-climate events especially at 8, 3.2, 2.2 and 0.4 Ma. The new record indicates similar stages in the development of the East and South Asian monsoons, with an enhanced winter monsoon over East Asia being the major difference. The rich spectrums of monsoon variability from the southern SCS also reveal other characteristic features of the low latitude ocean. Evidence for the evolution of the SCS includes the hemipelagic Oligocene sediments, implying the existence of deep water environments during the early seafloor spreading stage of the SCS basin. The four major unconformities and some remarkabl ediagenetic features in upper Oligocene deposits indicate the strongest tectonic events in the region. From a careful comparison of lithologies and sedimentation rates, we conclude that the prominent differences in sedimentary environments between the southern and northern SCS were established only by ～3 Ma.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

微孔草种子化学成分的研究

从微孔草 (MicroulasikkimenisHemsl)种子中首次分离出 8个已知化合物 :β 谷甾醇 (1)、羽扇豆醇 (2 )、白桦脂醇 (3 )、豆甾 4 烯 3 酮 (4)、豆甾 4 烯 3 二酮 (5 )、羟基豆甾 4 烯 3酮 (6)、诺米林 (7)、如忒文 (8) .报道了这8个化合物的分离纯化和结构鉴定     

微波诱导合成5-芳氧亚甲基-2-[5′-(4″-氯代苯基)- 2′-呋喃甲酰胺基]-1,3,4-噁二唑

在微波辐射条件下,由1 芳氧乙酰基 4 [5′ (4″ 氯代苯基) 2′ 呋喃甲酰基]氨基硫脲(1a～1i)在醋酸汞存在下,快速环合合成了一系列5 芳氧亚甲基 2 [5′ (4″ 氯代苯基) 2′ 呋喃甲酰胺基] 1,3,4 二唑(2a～2i).     

口服转胸腺素α1基因聚球藻对小鼠T细胞亚群作用的研究

为探讨口服后转胸腺α1(Tα1)基因聚球藻对T细胞亚群的作用,将小鼠随机分为空白对照组、野生藻组、转化藻(小剂量、中剂量和大剂量)组和日达仙组,用灌服的方式,给予空白对照组20mL·kg~(-1)的生理盐水,野生藻组0.4g·kg~(-1)的野生聚球藻;转化藻小、中、大剂量组,分别0.2g·kg~(-1)、0.4g·kg~(-1)和0.6g·kg~(-1)的转Tα1基因聚球藻;日达仙组则肌注给予3.2mg·kg~(-1)的胸腺素α1(日达仙)。结果表明转Tα1基因藻口服后可显著提高CD3亚群水平,显著提高CD4亚群水平,明显提高CD4/CD8的比值。提示转Tα1基因聚球藻具有增强机体免疫功能的作用。