A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

信号与系统中的matlab程序

在现行的理工科教学中,matlab的应用已经成为一个重要的内容。尤其在电子类的核心专业课信号与系统中,matlab的作用更加值得教师关注。文章就信号与系统这一课程中牵涉到的一些典型matlab程序,进行了注解和说明。试图在信号与系统和matlab的应用两方面找到了一个更为恰当的结合点,使学生和教师在专业知识和应用性工具方面,得到一个更好的处理方式。     

基于循环经济模式的交通运输可持续发展研究

文章阐明了循环经济的基本概念和基本理论，在分析我国交通运输业可持续发展面临的诸多问题后，结合循环经济模式对我国交通运输可持续发展战略进行了研究。     

Multiple MIPS 4Kc cores based interrupt controller design and its implementation on HDTV SoC platform

A multiple MIPS 4Kc processor cores based interrupt processing system is introduced. The interrupt controller plays a key role in the high definition television （HDTV） system-on-a-chip （SoC） platform, especially when it is a multiple processor system. Based on a general introduction to the whole HDTV SoC platform, a layered interrupt controller and its implementation are discussed in detail. The proposed scheme was implemented in our FPGA verification board. The results indicate that our scheme is reliable and efficient. Meanwhile, as a functional intellectual property （IP）, the interrupt controller has reusability and expandability with the layered structure.     

寻找精神家园的“歌手”——《浮士德》探幽

歌德的《浮士德》是“一种生活的发现艺术”。通过阅读作品，我们就会发现无论是“浮士德”形象还是“浮士德精神”，其最终的指向都是人的心灵世界，是人类追求和寻找精神家园的一种艺术化表达，浮士德的一生是寻找精神家园的一次审美体验。     

态叠加原理及其在结构化学上的应用

本文对态叠加原理的物理实质进行了讨论。又重点地介绍了态叠加原理在结构化学上三个方面的应用。     

冷拉拔对LY12铝合金小棒组织和性能的影响

研究了淬火前不同冷变形量对ＬＹ１２铝合金挤压小棒组织和性能的影响．结果表明：不同的冷拉拔量对ＬＹ１２铝合金组织和性能有明显的影响；适当的冷变形量能减少或消除该合金挤压棒材的粗晶环．作者还对粗晶环的形成原因及其消除方法进行了讨论．     

二维正态分布矩形区域概率质量的局部单调性

研究了二维正态分布矩形区域概率质量当矩形区域中心沿某特定方向平移时的局部单调性。同时给出了一簇矩形区域,其中任一矩形区域的概率质量对正态分布相关系数的绝对值具有局部单调性。     

面向对象的MIS重构

在MIS(Management Information System)的开发中,软件重用是提高开发效率的一条重要途径。本文通过引入对象的表示、变换等,提出了软件重构的概念和方法,它是对软件重用的重要扩充。     

论短跑摆腿技术的特点

近年来，随着田径运动技术水平的提高和发展，短跑的技术也有所变化，更加强调摆腿技术的重要性。摆腿的速度、力量、方向、幅度都直接影响着后蹬的效果。只有加强摆腿技术，才能更好地促进后蹬，只有提高蹬、摆的配合效果，才能更快，更好地提高运动成绩。