Fragile X mental retardation protein interacts with TDG

Fragile X syndrome is the most common form of inherited mental retardation disease, resulting from absent of expression of its disease geneFMR1. To study the function of the fragile X mental retardation protein (FMRP) through protein/protein interaction, a mouse embryo cDNA library was screened by the yeast two-hybrid system. A clone was found to interact specifically with FMRP. The cDNA of this clone (Genbank accession number af 102875) encoded a protein highly homologous to human G/T mismatch-specific DNA thymine glycosylase (hTDG). Interactions between various alternatively spliced FMRP isoforms and a series of mTDG deletion proteins were further studied in the yeast two-hybrid system and their interaction amino acid regions were determined. Interaction between FMRP and TDG existed inside exon 13 of FMRP (amino acid residue 397–425) and around amino acid residue 122–346 of TDG. These results will be helpful to the study of the biological role of FMRP.     

Lanthanides’ enhancing absorption of insulin and reduction of blood glucose of rat by pulmonary administration

To explore the possibility of lanthanides as the enhancer of insulin absorption, the promoting effects of lanthanide ions on the absorption of intrapulmonary delivered insulin and reduction of blood glucose were investigated by means of two approaches, preadministration and coadministration of lanthanide ions with insulin. The results indicate that, compared with the results of those given insulin only, lanthanide ions can effectively enhance the level of insulin and the reduction of glucose level in blood. These effects are dependent on lanthanide species and their concentrations. The mechanism was discussed.     

Inheritance of resistance toHelicoverpa armigera of 3 kinds of transgenicBt strains available in upland cotton in China

There are 3 kinds of transgenicBt strains, Shanxi 94-24, Zhongxin 94, and R19, in upland cotton in China. Their transgenicBt insect-resistance cultivars or hybrids have been developed and grown by farmers. Genetic studies indicate that the resistance of the 3 transgenicBt cotton strains toHelicoverpa armigera is controlled by one pair of non-allelic dominant genes. Linkage relationship between the resistant genes of R19 and Shanxi 94-24 transgenicBt strains shows that they may be inserted in the same chromosome. F₁ hybrids crossed among the 3 strains show that high levels of protection from feeding damage are the same as that of their parents. Therefore, there is no co-suppression phenomenon in many transgenic plants. The results presented here afford a fundamental reliance in developing transgenicBt insect-resistant cultivars and exploiting the heterosis of hybrids in upland cotton.     

二维应力分析的虚拟体力法

对于二维弹性问题,将虚拟体力设置在城外的假想边界上,采用二次插值对虚拟体力进行离散。根据弹性问题的Ｋｅｌｖｉｎ基本解,建立了虚边界积分方程,推导了各类边值问题的数值求解公式。算例表明,与传统边界元方法相比,本文方法求解思路简洁;与配点法相比,本文方法仅需少量的单元即可获得较高的数值精度。     

ROPS/FOPS弹塑性变形计算方法的研究

ROPS/FOPS(Roll Over Protective Structure/Falling Object Protect Structure)是加装在工程车辆驾驶室外的一种装置 ,其主要功能是在车辆发生滚翻与受落物冲击时有效地保护司机的生命安全。本文推导了弹性、弹塑性阶段 ROPS/FOPS变形的计算公式 ,编制了相应的计算机程序 ,计算了 TY32 0马力推土机的 ROPS/FOPS弹塑性变形量。     

鸡肾型传染性支气管炎的病理形态学观察

:剖检 2 0只鸡肾型传染性支气管炎病的病死鸡 ,分别取气管、肺脏、心脏、肝脏、肾脏、脾脏和肠管等器官 ,采用石蜡包埋切片法 ,H E染色法 ,在显微镜下进行了病理组织学观察。结果显示 :实质器官为渗出性炎症 ;上皮细胞肿胀、变性、坏死和血液循环障碍     

甲烷部分氧化制合成气反应中热点位置的研究

将热电偶插入La2 O3 Ni/MgAl2 O4 催化剂反应床层 ,通过移动热电偶详细考察了反应炉温、空速、原料气组成和加入水蒸气对甲烷部分氧化制合成气反应床层温升的影响。结果表明 ,反应热点位于催化剂床层入口处 ,而且热点位置不受上述反应因素的影响。但是 ,其温升值随空速的增大而升高 ,随反应炉温的升高而降低。反应中引入水蒸气可以降低反应热点的温度 ,但同时使产物中CO2 含量增大。根据反应热计算了燃烧重整机理和直接转化机理的绝热温升 ,与实验结果的对比表明 ,反应床层的温升主要来自于部分氧化反应的放热 ,即甲烷部分氧化制合成气可能是按直接转化机理进行的。极高空速时低甲烷转化率下合成气的选择性并未发生明显的变化 ,这也进一步证明了上述结论     

利用遗传算法进行振动吸振器的优化设计

为了解决振动吸振器优化设计的非线性多维离散问题,引入了全局搜索遗传算法,以频率范围内的声功率峰值最小作为目标函数,对吸振器的位置及结构参数进行了优化设计。采用了混合编码与ｓｔｅａｄｙｓｔａｔｅ算法操作,以提高遗传算法的性能,用位移响应基础法替代传统的有限元方法计算了声功率值,节约了遗传算法的计算时间。最后以悬臂板梁为例,对算法进行了验证。     

哺乳动物神经纤维的双向脉冲选择性刺激研究

为了研究神经纤维的双向脉冲选择性刺激方法,对神经纤维的各种物理模型进行了研究和比较,建立了哺乳动物有髓神经纤维的仿真系统,并利用该系统对以前工作中提出的３种双向脉冲的选择性刺激方法（单电极,双电极和三电极方法）进行了研究,发现也可以实现复合神经干中细神经纤维的选择性兴奋,证明这些方法同样适用于哺乳动物的神经纤维模型。     

ELISA对毒理实验动物血清中神经生长因子抗体动态的研究

用ＥＬＩＳＡ法对神经生长因子（ＮＧＦ）毒理实验动物犬和大鼠血清作了抗－ＮＧＦ抗体检测。结果表明在大鼠血清中没有发现抗－ＮＧＦ抗体,但犬血清中有相应的抗体产生,并随注射剂量增加和注射时间延长而逐渐升高,另外,对ＥＬＩＳＡ间接法和竞争抑制法进行了比较,结果表明,竞争抑制法明显优于间接法,而且是一种简便、特异的好方法。