ESD模拟器电流波形校验装置

分析了静电放电模拟器的实验原理,研制成功符合国际电工委员会ＩＥＣ６１０００４－２标准的ＥＳＤ模拟器电流校验系统,该系统的自动采集ＥＳＤ电流波形,计算上升时间、峰值电流、３０ｎｓ时电流、６０ｎｓ时电流等参数,该装置还可广泛应用于其它形式静电放电电流测量和静电放机理研究。     

Effect of monoclonal antibody to bull sperm sp18 on murine fertilization and embryos development

Western blot analysis revealed that one IgG1 monoclonal antibody (mAb) to sp18 family membrane proteins (Mr. 14, 16 and 18 ku) of bovine sperm reacted faintly with protein bands of 14, 18, 22, 30 and 60 ku (reducing) in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence (IIP) showed that the sp18 antigens were present in the posterior head of murine sperm. In murine in vitro fertilization (IVF) and embryo development trails, a total of 426 oocytes from C57BL/6 and F1 hybrid strain (CD1 × C57BL/6 cross) of 12 female mice were used in 3 independent trails. After preincubating capacitated sperm with 182 μg/mL of sp18 mAb in the modified TYH IVF medium for 15-20 min, cumulus-oocyte-complexes were introduced. The fertilization rate in sp18 mAb groups was 77.1 %, which was not significantly (P > 0.05) different from the nonspecific mouse IgG (79.2%) and non-IgG (80.3 %) control groups. Fertilized oocytes had been continuously cultured in modifed CZB medium. 100%, 100% and 97.9% of 1-cell embryos developed to 2-cell stage in sp18 mAb, nonspecific mouse IgG and non-IgG group 30 h after the start of fertilization, respectively. In the nonspecific mouse IgG and non-IgG groups, 64.1 % and 64.3% of embryos developed to the 4-cell stage, respectively, but all developing eggs in sp18 mAb groups arrested development in vitro at 2-cell stage. After zonae of 2-cell blocked embryos were enzy-matically removed with 0.5% pronase, detection of sp18 antigens by IIF indicated that the fluorescence scattered on two embryonic cells. For embryos fertilized in vivo and co-cultured with 182 μg/mL sp18 mAb, the numbers of 1-cell embryos reaching the 2-cell and 4-cell stage were 95. 2% and 70. 5%, which were not significantly (P>0.05) different from the control group (92.9% and 77.9%). These results indicate that the sp18 antigens on posterior head of mouse sperm were incorporated into the egg plasma membrane during fertilization, and played an active role in development of murine preimplanta-tion embryo.     

菜心多倍体诱变及其细胞学观察

报道了用秋水仙素水溶液处理菜心种子及幼苗生长点获得多倍体的方法,并对获得的四倍体与二倍体（ＣＫ）进行了形态学、细胞学以及结籽率等的比较观察。试验结果表明：（１）在１０～１５℃的条件下,用０．１％秋水仙素水溶液处理幼苗生长点４８小时的诱变效果最好,诱变率可达２６．６７％,浸泡种子效果不佳,最高诱变率仅为４．０％。（２）对四倍体植株分别进行人工自交均获得了种子,但结籽率较二倍体（ＣＫ）明显降低,平均达４２．３％,而八倍体植株几乎是完全不育的。（３）与二倍体（ＣＫ）比较,四倍体植株生长旺盛,花薹的产量和品质均有所提高。（４）随着染色体组的增加,花粉粒大小和叶片保卫细胞中的叶绿体数均显著增加,可作为鉴定植株倍性的一个指标     

基于Web的远程教育课程表的设计与实现

主要介绍了一个基于Ｗｅｂ的远程教育课程表的功能设计与实现细节·重点阐述了使用Ｊａｖａ语言进行网络通信与交互控制的技术方法·使用了ＪａｖａＪＤＫ１－０２开发环境·其中,日历的绘制可根据设置方法中的相应参数来适应不同的月份;它通过ＵＲＬ读取数据文件,而不依赖于具体的主机;对数据的修改可通过浏览器,调用ＣＧＩ程序完成,充分发挥了Ｊａｖａ的网络编程优势,并具有良好的可移植性·     

广义SRLW方程的Fourier谱方法

考虑了一类广义的对称正则长波方程的周期初值问题。对所论问题构造了不同于已有文献的半离散和全离散的Ｆｏｕｒｉｅｒ－Ｇａｌｅｒｋｉｎ谱格式,采用更精细的估计不等式,给出了近似解的收敛性证明和最佳误差估计,改进了已有文献的结果。     

黄精凝集素Ⅱ对大鼠主动脉Ca^2＋跨膜流动的影响

用４５Ｃａ跨膜流动技术测定黄精凝集素Ⅱ对大鼠主动脉Ｃａ２＋跨膜内流的影响．黄精凝集素Ⅱ在０．１～１０μｍｏｌＬ－１时,不影响静息状态下Ｃａ２＋的内流,但能抑制ＮＥ和ＫＣｌ引起的Ｃａ２＋内流,其抑制能力依赖于凝集素的浓度;在１０μｍｏｌＬ－１时,可完全阻滞ＮＥ引起的Ｃａ２＋增加,并能有效地阻滞ＫＣｌ引起的内流作用．表明黄精凝集素Ⅱ能阻滞细胞外的Ｃａ２＋通过受体操纵钙通道（ＲＯＣ）和电压依赖钙通道（ＰＤＣ）的内流     

新集二矿选煤厂使用动筛跳汰机工艺流程的设计

介绍了新集二矿选煤厂使用动筛跳汰机工艺流程的设计特点及实际应用效果     

空间数据模型研究

讨论几种常用的二维和三维空间数据模型,逐一介绍镶嵌模型、矢量模型、混合模型和分析模型的建模方式,并分析了它们的主要优缺点。     

破坏准则的描述新方法

提出了一种描述破坏准则的新方法。它特别适合对混凝土破坏准则的描述。各种应力组合只能在一个有限的平行四边形区域上变化。采用这种描述方法,能够指导试验数据的选取和破坏曲面的拟合。     

论科技创新的基石--科学数据共享

科技创新是人类社会发展和变革的主导力量。本文在阐述科技创新内涵和分析数据、信息、知识、智力四者关系的基础上 ,提出科学数据是科技创新和国家发展的战略资源 ,科学数据共享是时代发展的必然