Logical computation using algorithmic self-assembly of DNA triple-crossover molecules

Recent work has demonstrated the self-assembly of designed periodic two-dimensional arrays composed of DNA tiles, in which the intermolecular contacts are directed by 'sticky' ends. In a mathematical context, aperiodic mosaics may be formed by the self-assembly of 'Wang' tiles, a process that emulates the operation of a Turing machine. Macroscopic self-assembly has been used to perform computations; there is also a logical equivalence between DNA sticky ends and Wang tile edges. This suggests that the self-assembly of DNA-based tiles could be used to perform DNA-based computation. Algorithmic aperiodic self-assembly requires greater fidelity than periodic self-assembly, because correct tiles must compete with partially correct tiles. Here we report a one-dimensional algorithmic self-assembly of DNA triple-crossover molecules that can be used to execute four steps of a logical (cumulative XOR) operation on a string of binary bits.     

Hot-spot residue in small heat-shock protein 22 causes distal motor neuropathy

Distal hereditary motor neuropathies are pure motor disorders of the peripheral nervous system resulting in severe atrophy and wasting of distal limb muscles. In two pedigrees with distal hereditary motor neuropathy type II linked to chromosome 12q24.3, we identified the same mutation (K141N) in small heat-shock 22-kDa protein 8 (encoded by HSPB8; also called HSP22). We found a second mutation (K141E) in two smaller families. Both mutations target the same amino acid, which is essential to the structural and functional integrity of the small heat-shock protein alphaA-crystallin. This positively charged residue, when mutated in other small heat-shock proteins, results in various human disorders. Coimmunoprecipitation experiments showed greater binding of both HSPB8 mutants to the interacting partner HSPB1. Expression of mutant HSPB8 in cultured cells promoted formation of intracellular aggregates. Our findings provide further evidence that mutations in heat-shock proteins have an important role in neurodegenerative disorders.     

Cloning of the gene for a human dopamine D5 receptor with higher affinity for dopamine than D1.

Dopamine receptors belong to a superfamily of receptors that exert their biological effects through guanine nucleotide-binding (G) proteins. Two main dopamine receptor subtypes have been identified, D1 and D2, which differ in their pharmacological and biochemical characteristics. D1 stimulates adenylyl cyclase activity, whereas D2 inhibits it. Both receptors are primary targets for drugs used to treat many psychomotor diseases, including Parkinson's disease and schizophrenia. Whereas the dopamine D1 receptor has been cloned, biochemical and behavioural data indicate that dopamine D1-like receptors exist which either are not linked to adenylyl cyclase or display different pharmacological activities. We report here the cloning of a gene encoding a 477-amino-acid protein with strong homology to the cloned D1 receptor. The receptor, called D5, binds drugs with a pharmacological profile similar to that of the cloned D1 receptor, but displays a 10-fold higher affinity for the endogenous agonist, dopamine. As with D1, the dopamine D5 receptor stimulates adenylyl cyclase activity. Northern blot and in situ hybridization analyses reveal that the receptor is neuron-specific, localized primarily within limbic regions of the brain; no messenger RNA was detected in kidney, liver, heart or parathyroid gland. The existence of a dopamine D1-like receptor with these characteristics had not been predicted and may represent an alternative pathway for dopamine-mediated events and regulation of D2 receptor activity.     

一种基于事务冲突图的并发控制方法

提出了一种基于事务冲突图的并发控制方法．事务在执行前要提交其读集和写集，系统根据事务的读集和写集构造出一无圈的事务冲突图，并根据事务冲突图进行并发控制．这种并发控制方法具有对数据库无结构要求、无死锁、非两阶段等优点．讨论了其集中式并发控制方法和分布式并发控制方法．     

分布式选举算法

提出了一种基于任意网络拓扑的分布式选举领导人算法。每一处理机既不知道系统的网络拓扑，也不知道系统中处理机的台数。对于由ｎ台处理机组成的分布式系统，算法的消息复杂度最好为３＃Ｅ，最坏不超过＃Ｅ（２Ｎ＋１）－Ｎ＋ｎ；对于同步分布式系统，其时间复杂度最好不超过３（ｎ－１）△ｔ，最坏不超过４（ｎ－１）△ｔ。     

基于广义能量法的曲面光顺

提出了同时考虑曲面几何及物理等多项光顺准则的基于广义能量法的曲面整体光顺思路，建立了包含曲面截线族粗光顺、曲面粗光顺两个步骤的曲面光顺方法，论述了曲面精光顺中广义能量表达式和约束条件的建立，实践证明，这种方法对于提高曲面的整体光顺性能具有良好的效果。     

数控设备E-服务技术研究

针对数控设备售后服务与技术支持问题,提出基于Web的服务保障系统．系统由E-服务平台、数控机床网关和远程终端三部分组成．E-服务平台是一个特殊网站,它通过Internet向客户提供技术支持与服务．数控机床网关是一个移动PC,当机床发生故障时将其带到现场,采集机床PLC信息、视频信息和传感器信息,并将信息传送到E-服务平台供诊断分析．远程终端可以是连接在Internet上的任何PC机,客户通过远程终端访问E-服务平台,获得快速和高质量的产品售后服务．