BRD7 regulates the insulin-signaling pathway by increasing phosphorylation of GSK3β

Reduced hepatic expression levels of bromodomain-containing protein 7 (BRD7) have been suggested to play a role in the development of glucose intolerance in obesity. However, the molecular mechanism by which BRD7 regulates glucose metabolism has remained unclear. Here, we show that BRD7 increases phosphorylation of glycogen synthase kinase 3β (GSK3β) in response to activation of the insulin receptor-signaling pathway shortly after insulin stimulation and the nutrient-sensing pathway after feeding. BRD7 mediates phosphorylation of GSK3β at the Serine 9 residue and this effect on GSK3β occurs even in the absence of AKT activity. Using both in vitro and in vivo models, we further demonstrate that BRD7 mediates phosphorylation of ribosomal protein S6 kinase (S6K) and leads to increased phosphorylation of the eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) and, therefore, relieves its inhibition of the eukaryotic translation initiation factor 4E (eIF4E). However, the increase in phosphorylation of 4E-BP1 with BRD7 overexpression is blunted in the absence of AKT activity. In addition, using liver-specific BRD7 knockout (LBKO) mice, we show that BRD7 is required for mTORC1 activity on its downstream molecules. These findings show a novel basis for understanding the molecular dynamics of glucose metabolism and suggest the unique function of BRD7 in the regulation of glucose homeostasis.     

Genome-wide association studies of 14 agronomic traits in rice landraces

Uncovering the genetic basis of agronomic traits in crop landraces that have adapted to various agro-climatic conditions is important to world food security. Here we have identified ～ 3.6 million SNPs by sequencing 517 rice landraces and constructed a high-density haplotype map of the rice genome using a novel data-imputation method. We performed genome-wide association studies (GWAS) for 14 agronomic traits in the population of Oryza sativa indica subspecies. The loci identified through GWAS explained ～ 36% of the phenotypic variance, on average. The peak signals at six loci were tied closely to previously identified genes. This study provides a fundamental resource for rice genetics research and breeding, and demonstrates that an approach integrating second-generation genome sequencing and GWAS can be used as a powerful complementary strategy to classical biparental cross-mapping for dissecting complex traits in rice.     

Tl-Ba-Ca-Cu-O系超导体相结构和掺Mn影响的电镜研究

Tl-Ba-Ca-Cu-O系超导体多相同时存在,多至6相,均属四方晶系钙钛矿结构,其中(2223)(2212)相共生,其衍射谱给出c≈33A的假象.加Mn可以部分取代Tl,但增加Mn的含量将失去高温超导性.     

Effect of matrix metallo-proteinase-26 (MMP-26) during embryo implantation in the mouse

Matrix metalloproteinase-26 (MMP-26, endometase and matrilysin-2), a novel member of the MMPs family, is detected not only in the placenta and uterus, but is widely expressed in malignant tumors from different sources as well as in diverse tumor cell lines. However, the function of MMP-26 in the reproductive system has never been reported. Expression of MMP-26 in mouse embryos and the function of the MMP-26 antibody during mouse embryo implantation was examined for the first time by injecting the uterine horn, immunohistochemistry,in situ hybridization, co-culture of mouse blastocysts and uterine monolayer epithelial cells, Western blot, RT-PCR, Northern blot and zymography. Our results show that there is strong expression of MMP-26 mRNA and protein in the mouse embryo. Furthermore, the MMP-26 antibody dramatically inhibited mouse embryo implantation and significantly inhibited adhesion and outgrowth of mouse blastocysts onin vitro uterine monolayer epithelial cells. At the same time, the MMP-26 antibody inhibited the expression of integrin αV mRNA and protein in a dose-dependent manner. These data suggest that MMP-26 may play a role in some of the tissue-remodeling events associated with the invasion of the endometrium by trophoblast cells and facilitate successfully embryo implantation.     

基质金属蛋白酶-26(MMP-26)在小鼠胚胎植入过程中的作用

基质金属蛋白酶-26（MMP-26，又称Endometase或基质水解素-2）是MMPs家族的一个新成员，它可在胎盘，子宫以及不同肿瘤细胞系中表达，采用子宫角注射，免疫组化，原位杂交，胚泡与子宫上皮单层培养，免疫印迹，RT-PCR和RNA印迹等多种体内外实验方法研究并报道了MMP-26在小鼠胚胎中的表达以及MMP-26抗体在胚胎植入中的作用，研究表明，MMP-26的mRNA与蛋白在小鼠胚泡中均有强烈的表达，此外，体内研究显示，MMP-26抗体能显著抑制小鼠胚胎的着床；在体外培养体系中，MMP-26抗体可显著抑制小鼠胚泡在子宫上皮单层上的黏附与扩展；同时，MMP-26抗体以剂量依赖关系抑制整合素αV亚基mRNA和蛋白的表达，研究提示，MMP-26可能直接或间接参与滋养层细胞侵入子宫内膜的过程，促使小鼠胚泡成功植入。     

采绒革盖菌锰过氧化物酶的诱导及部分特性研究

研究了不同碳源、氮源对采绒革盖菌锰过氧化物酶分泌的影响及锰过氧化物酶的部分特性 ;结果表明 ,淀粉为碳源 ,玉米粉为氮源有利于锰过氧化物酶的分泌 ;在 30℃、110r/min恒温振荡培养条件下 ,第 9天达产酶高峰 ,峰值酶活 345 2u/ml;酶作用的最适pH为 4 0 ,最适温度 30℃ ,Mg2 、Mn2 等离子对锰过氧化物酶有激活作用 ,Ag 、Cl- 等离子对酶活有明显的抑制作用 .     

The Distribution of a CP-asymmetric quantity in the decay channel J / Ψ → π + π ? π 0

The distribution of a CP-asymmetric quantityA in the decay channelJ/ψ→π ⁺ π ⁻ π ⁰ is investigated. This dimensionless quantityA is constructed from the momenta of the π-meson final states, namely A=P _x ^{π +} P _y ^{π −} -P _x ^{π −} P _y ^{π −}, whereP _h,P _x ^h andP _y ^h are the module of the momentum of a hadron h, the x- and y-components of that momentum respectively, theJ/ψ particle is produced by e⁺ e⁻ collision, and the direction of the momentum of e⁺ is taken to be the positive direction ofz axis. There would exists the violation under the combined transformation of charge conjugation and space reflection (CP violation) when the average ofA among a lot of events, 〈A〉, be examined to be nonzero clearly from data. In this way, 748 events are selected from the BES experimental data, and analyzed. The corresponding averageA-value is measured to be 〈A〉 = 0.010 39 ± 0.014 61 ± 0.015 2. Further, some discussion of our result, the size of the data sample being need for further attempt and a possible perspective are given.     

一种有效的运动目标辨别方法

提出了一种利用图像序列中两帧相邻图像从分割所得二值图像的各个候选目标块中确定真实目标的有效算法．背景配准后，可获得背景噪声分布的直方图，同时获得具有对应关系的候选目标块间的噪声分布直方图，通过比较它们与背景噪声分布直方图之间的差异性，即可准确地辨别出真实目标．通过对实际图像序列进行实验，证实了本方法的有效性．     

Geochronology of source materials from high-pressure and ultrahigh-pressure metamorphic rocks in Jurassic sedimentary rocks of Hefei Basin

Eclogite cobbles were discovered by Wang et al. (2001) in the Fenghuangtai Formation of the northern margin of Dabie Mountains in the Dushan area of Anhui Province, China[1]. They proposed that after the formation during the Triassic, the high-pressure (HP) and ultra-high-pressure (UHP) metamorphic rocks reached the sur-face through exhumation and uplifting before the late Ju-rassic. However, they have not provided any evidence about isotopic ages. One kind of cobbles of HP-UHP rocks w…