A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

矿用支护托梁复合材料π型带的结构设计与有限元分析

通过对W型钢带的结构和材料的改进,设计出复合材料π型带,并与W型钢带进行了有限元分析比较,得出了复合材料π型带优于w型钢带的结论.     

寻访最后的“吃素人”——云南省文山州“吃素人”习俗考察

“吃素人”指的是那些在新中国建立前由于种种原因在年轻时不愿出嫁或难以出嫁，而后终身不嫁并食素茹菜、念经拜神的女性。“吃素人”承袭着不同于其他人群的某些信仰和生活习俗。她们信奉观音、韦驼等佛教之神及土地神、捧表仙官等道教之神，但没有统一的组织和形式。她们不是严格意义上的道教徒或佛教徒。从吃素人的信仰习俗来看，体现了民间流传的佛、道两教的合流与融合的现象。     

MAPLE在微积分教学中的应用

极限是微积分的重点和难点。本文简述了数学软件MAPLE在极限教学中的应用。     

一些新的序压缩映射的不动点定理

在序Banach空间中,利用锥理论和单调迭代技巧对序压缩映射作了进一步的研究,对作用在序区间上的压缩映射给出了几个新的形式,并证明了相应的唯一不动点定理.     

后湾水库除险加固方案分析

探讨了后湾水库除险加固工程的原枢纽方案和原枢纽加泄洪洞方案,通过对工程地质、施工条件、运行管理、工程投资等多方面综合分析,最终选用了原枢纽方案。     

移动通信系统安全机制发展的研究

从移动通信系统演进的角度介绍了安全机制的发展历程,分析研究了2G的安全性措施,阐述了3G认证签权方案及其优缺点,并对移动通信安全机制提出了一些见解。     

Amniotic membrane transplantation for porous sphere orbital implant exposure

Objective: This study is aimed at describing the clinical outcome of amniotic membrane transplantation for exposure of porous sphere implants. Methods: A retrospective review of consecutive cases of porous sphere orbital implant exposure was carried out. Eight cases were presented between May 2004 and Oct. 2006 (5 males, 3 females; mean age 44.5 years). Six had enucleation and two had evisceration. Exposure occurred in two primary and six secondary. Orbital implant diameter was 22 mm in seven cases and 20 mm in one case. Six patients are with hydroxyapatite and two with high-density porous polyethylene (Medpor) orbital implants. The mean time from implantation to exposure was 1.1 months (range 0.8~2 months). All patients required surgical intervention. Results: The time of follow-up ranged from 3.0 to 28.0 months (mean 16.5 months). Amniotic membrane grafting successfully closed the defect without re-exposure in all of these patients. The grafts were left bare with a mean time to conjunctiva of about 1 month (range 0.8~1.5 months). Conclusion: Exposed porous sphere implants were treated successfully with amniotic membrane graft in all of patients. The graft is easy to harvest. This technique is useful, dose not lead to prolonged socket inflammation and infection, and it is valuable application extensively.     

浅谈利用任务驱动法进行单片机教学与职业核心能力的培养

本文就任务驱动法在单片机教学中的应用与职业核心能力培养进行了阐述，并且以实例的形式得到了很好的验证。     

对五棵树开发区区域环境的研究

通过对建设五棵树开发区周边环境状况影响评价，指出影响环境因素。根据周边环境质量现状，给出建立综合治理污染措施及手段，并对开发区建设后的生态适宜性进行了研究。