Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system

The human adenovirus type 5 E1A, a tumor- suppressor gene[1], codes for two major related proteins of 243 amino acids (12S) and 289 amino acids (13S) by al-ternative splicing in two exons[2]. Studies have been shown that E1A can regulate expression of many genes and cell cycle[3]. Both in vitro and in vivo experiments indicated that E1A could induce tumor cells differentia-tion, convert tumor cells into an epithelial phenotype, in-hibit tumor cell growth and metastasis and strongly en-ha…     

A model for assessing effects of climate change on runoff in China

A model is established for assessing the effects of climate change on runoff in China based on the land surface parameterization scheme variable infiltration capacity (VIC). The entire area of China is represented by 2604 cells with a resolution of 60 km×60 km for each cell. Forcing data, soil and vegetation parameters needed by the VIC model for the entire area of China are prepared. Daily forcing data, which are obtained from 740 stations between 1980 and 1990, are interpolated to the 60 km×60 km grid system. The VIC model is run on every grid cell over the whole China, and a routing scheme is run offline with daily input of surface runoff and drainage from the VIC to get hydrograph at basin outlets. The spatial patterns of simulated runoff and mean annual precipitation are consistent very well. The results of monthly streamflow simulations over the Huaihe and Weihe River basins indicate that there is a good agreement between the observed and simulated values, and also initially indicate the rationality and feasibility of the evaluation model.     

A dynamic fuzzy clustering method based on genetic algorithm

A dynamic fuzzy clustering method is presented based on the genetic algorithm. By calculating the fuzzy dissimilarity between samples the essential associations among samples are modeled factually. The fuzzy dissimilarity between two samples is mapped into their Euclidean distance, that is, the high dimensional samples are mapped into the two-dimensional plane. The mapping is optimized globally by the genetic algorithm, which adjusts the coordinates of each sample, and thus the Euclidean distance, to approximate to the fuzzy dissimilarity between samples gradually. A key advantage of the proposed method is that the clustering is independent of the space distribution of input samples, which improves the flexibility and visualization. This method possesses characteristics of a faster convergence rate and more exact clustering than some typical clustering algorithms. Simulated experiments show the feasibility and availability of the proposed method.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

钢管混凝土拱桥的地震响应分析方法

目前常用于钢管混凝土拱桥的地震响应分析方法有换算截面法和双单元法 .提出一种基于钢管混凝土统一理论 ,将钢管混凝土作为一种材料 ,根据其构件力学性能指标进行地震响应分析的方法 ,并运用这 3种方法对一钢管混凝土拱桥实例进行了分析 .比较分析其计算结果 ,认为基于统一理论的方法是一种分析方便易行的钢管混凝土拱桥地震响应分析方法     

螺杆冷水机组稳态仿真

为预测螺杆冷水机组在大范围结构参数以及运行工况下的性能 ,提出了该类相应的稳态仿真模型 .模型由一系列部件模型组成 ,包括压缩机、经济器、膨胀阀、壳管式冷凝器以及满液式蒸发器 .结合顺序模块方法和连续替代方法实现了模型的求解 .模型预测结果与不同规格的 4台不带经济器冷水机组和 3台带经济器冷水机组的实验数据相比 ,误差在± 1 0 %以内 .通过模型验证发现 ,只有当压缩机一级内压缩容积比大于一定值时 ,机组才能获得高于其不带经济器时的 COP     

电容式微机械加速度计部分力学特性的分析

推导了电容式微机械加速度计的悬臂梁在加速度作用下可能出现的两种挠度情况，分析了在制造过程中残余应力对于加速度计造成的影响，推导了电容式微加速度计的工作原理，并推荐了相应的工作模式．     

带有预算费用约束的多地点报童模型

有针对性地研究了一种带有预算费用约束的多地点(连锁店)报童问题。在各地需求变量均服从正态分布的情况下,建立了数学模型,利用最优化方法进行了求解,并对模型的灵敏度进行了分析。     

压力平衡拱应力状态相似物理模拟研究

相似材料模拟是通过建立相似物理模型，观测研究难以用数学描述的系统特性，研究原型系统与模拟系统间相似特性的。模型与原型的区别，仅在系统要素的序结构和特征值有一定的比例大小，而支配系统特性的本质不变。从系统仿真角度上说，建立相似的物理模型，可称为相似物理仿真模拟。通过相似材料模拟，对压力平衡拱处岩层的水平应力和竖直应力的观测。     

系统安全性预测模型与系统的建立

系统安全性预测对预防事故起到至关重要的作用，而预测模型的正确选择是预测的关键。本文就目前安全性预测的原则、预测模型的复杂程度、特点和选择等若干问题进行了分析，并就系统安全性预测中如何处理这些问题进行了探讨。在综合分析的基础上，建立了合理的预测系统。组合预测利用各种预测方法提供的有用信息，有效提高了预测精度，改变由于预测模型选择不合理而带来得预测误差。