Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

法的历史类型与国际法

本文从国际法的角度阐释了法的历史类型,并对中国法理学若干问题进行探讨.     

非公有制经济参与西部大开发若干问题的研究

我国经济发展机制的重要变化是非公有制经济迅速成长壮大,并以其特有的活力与生机成为经济发展的新的增长空间。注重和加强非公有制经济参与西部大开发的问题研究,对促进以国有经济为主导的西部地区经济高速发展,具有重要的理论意义和实际意义,并能为政府关于区域和西部开发的发展战略提供参考依据。     

苏云金杆菌库斯塔变种(HD-1)对菜青虫杀虫效果的研究

通过实验室饲料感染方法和大田喷杀对比试验研究，表明发酵培养基中添加花生饼可以提高HD—1的杀虫效果，且添加浓度与HD—1菌株毒力呈正相关，用添加了花生饼的固体浅盘发酵培养液比市售工业化生产的同类杀虫剂杀虫效果要高得多。     

产芽孢细菌B130（BACILLUS SPP）培养条件的初步研究

本文就产芽孢细菌B130(Bacillus spp.)培养条件进行了初步研究,筛选出产菌量较高且来源方便,价格低廉的培养基成分(％w/v):豆饼粉3,麸皮4,玉米粉3,最适pH:6.5或8.0,确定了最佳培养时间区间为平衡期末期。     

Erosion and Sediment Production in Small Watershed in Purple Hilly Areas and Prevention Techniques

Purple Soil distributes extensively and mainly in China. Because of abundant easily weatherable parent rocks/materials and unstable soil structure, and also influenced by parent materials, usage systems, and slope gradients, erosion and sediment production of purple soils are very severe with main fashions of water erosion and gravitational erosion. Basing on observed data in small watersheds, rainfall erosivity,vegetation coverage, previous soil water content, flow and relating factors such as climate, topograph of small watershed,land usage, and soil kinds are all the influence factors of erosion and erodibility of purple soil as well as sediment production and transport in small watershed of purple hilly areas.The effective technological countermeasures of ecosystem restoration, agricultural tillage for water conservation and erosion prevention, agriculture project, and soil changing for fertility and anti-erosion were provided.     

微量分析系统测定微量全血中的锌、铁、钙

提出了一种用微量分析系统测定微量全血中的锌、铁、钙的分析方法 ,该系统是利用软件来记录吸光度随时间的变化率 ,即导数原子吸收值来提高原子吸收分析灵敏度的一种新技术 .该方法灵敏度高 ,检出限低 ,重视性好 ,直接用于测定微量全血中的微量元素 ,结果满意     

AN IMPROVEMENT ON JORGENS'S ASYMPTOTIC EXMNSION THEOREM FOR TRANSPORT EQUATION

1.IntroductionAfundamentalproblemintransporttheoryisthedeterminationoftheasymptoticbehaviorofthetimedependentsolution.In[if,Jorgenssystematicallyinvestigatedtheneutrontransportequationinafiniteconvexbodywithenergyboundedawayfromtheorigin,andgaveanasymptot…     

新时期大学生成长观教育面临的主要问题

社会主义市场经济的建立和发展使人的主体性意识进一步增强,为大学生的发展提供了更加广阔的空间,对大学生的素质提出了更高的要求,也给教育工作者提出了新的要求.从个人才干增长与社会需要、奉献与索取、成长中的自由与必然三个方面阐述新时期大学生成长观教育中所面临的主要问题,以期在教育工作中做到有的放矢.     

在线固溶条件下的输出辊道受热分析

为满足300系列奥氏体不锈钢轧后在线目溶处理的要求，针对某厂已有设备条件，进行在线固溶处理输出辊道的改造，利用ANSYS有限元分析软件，通过对不同结构和材质条件下输出辊道的受热分析，为输出辊道的改造提供了理论依据。结果表明，将原有的铸铁辊道改为陶瓷辊道能够满足生产的要求。