Efficient expression of human factor Ⅸ cDNA in liver mediated by hydrodynamics-based plasmid administration

The transfection of plasmid DNA into mammalian cells is an indispensable tool in the study of gene transfer and gene function. Since the original report in 1990 on the successful expression of a reporter gene in muscle[1], plasmid DNA injection has been widely used to mediate gene transfer study. As a gene transfer vector, naked DNA has many obvious advantages. It is easy and cheap to be prepared and more safe after transfection. But the plasmid mediated gene expression is low because of t…     

Instability of the shear layer in the near wake of a circular cylinder

The instability of the shear layer separated from a circular cylinder is studied with the Reynolds number (Re) of 3000～104 by numerically solving the two-dimensional Navier-Stokes equations. In the wake of the cylinder, primary vortex shedding with natural frequency fs occurs, and the instability of the shear layer with frequency ft develops, which leads to mixing layer eddies and interacts with the primary shedding vortices. However, there remains some uncertainties regarding to the variation of the shear layer characteristic frequency with the Reynolds number. Based on the previous experimental work, several relationships of ft/fs with Re has been proposed including ft/fs～Re0.5 by Bloor, ft/fs～Re0.87 by Wei and Smith and ft/fs～Re0.67 by Prasad and Williamson. The objective of this study is to predict reasonably the relation of the shear layer instability frequency with the Reynolds number based on the present accurate calculation with the high-order schemes and high-resolution spectrum analysis. According to our calculated results, a variation for the normalized shear-layer frequency of the form ft/fs～Re0.69 is predicted numerically, which is in good agreement with a recent experimental measurement of Re0.67 and physical prediction of Re0.7.     

二维装配图的快递交点排序裁剪算法

提出一个任意多边形的快速交点排序线裁剪算法,该算法简单快捷,效率高,并将其成功用于工程装配图的二维消隐。解决了大多数算法将凹多边形裁剪分解为凸多边形处理存在计算时间长、难度大等问题。     

微孔草种子化学成分的研究

从微孔草 (MicroulasikkimenisHemsl)种子中首次分离出 8个已知化合物 :β 谷甾醇 (1)、羽扇豆醇 (2 )、白桦脂醇 (3 )、豆甾 4 烯 3 酮 (4)、豆甾 4 烯 3 二酮 (5 )、羟基豆甾 4 烯 3酮 (6)、诺米林 (7)、如忒文 (8) .报道了这8个化合物的分离纯化和结构鉴定     

机车蓄电池智能充放电监控系统

针对传统机车蓄电池检修及检修后充放电过程中存在的效率低、维护困难等问题,提出了一种集监测和管理于一体、采用PC机控制构建的智能蓄电池充放电监控系统,并对该系统的主要功能模块进行了分析.     

纳米结构V2O5薄膜的溶胶凝胶制备与特性研究

采用溶胶 -凝胶技术 ,以V2 O5粉末为原材料制备了纳米结构的V2 O5薄膜 .使用椭偏仪、X射线衍射仪(XRD)、红外分光光度计 (FTIR)、电化学分析、原子力显微镜 (AFM)等方法研究了V2 O5薄膜的特性 .实验结果表明 :薄膜具有纳米多孔结构 ;热处理使得薄膜致密 ,折射率提高 ,薄膜结晶 .红外吸收测量揭示了刚制备的薄膜中钒以 4价离子为主 ,高温热处理后形成 5价钒离子 ,相应出现了V2 O5特征吸收峰 .这种结构的薄膜具有很好的锂离子注入 /退出可逆性和很高的离子注入容量 ,可用作锂离子电池的高性能阴极材料 .     

豇豆属3种植物染色体的核型比较分析

采用去壁低渗火焰干燥Giemsa染色法,对小豆、绿豆、豇豆3种豇豆属植物核型进行了研究.结果表明,3种植物的染色体核型公式均为2n=2x=22=18m 4sm.所不同的是小豆和绿豆两条sm染色体分别为第2、3对,而豇豆的sm染色体则为第2和第6对.按照Stebbins核型分类方法,细胞类型均为2B.3种植物的染色体相对长度组成各不相同.     

批量图像叠加文字的程序处理

为解决批量图像上叠加文字的问题，比较了Photoshop批处理、GD图形库编程、Fire—worksJavaScript等几种大批量图像汉字叠加的程序处理方法，最后给出了一种利用ExcelVBA、以XML语言作为数据中介、以FireworksMX为图像处理工具的相对简便的解决方案。     

二维溃坝洪水波演进的数值模拟及动态显示

采用基于MacCormack预测—校正技术的隐式数值格式求解控制水流运动的二维浅水方程，建立了模拟大坝瞬间全溃或局部溃倒所致的洪水演进过程的数学模型；应用该模型对矩形明渠中缓、急流过渡的水面曲线进行了数值计算，并与理论解进行了比较；最后用该模型预测了矩形河道中大坝瞬间局部溃倒的洪水演进过程，并对模拟结果进行了定性分析。比较与分析结果表明，该模型对模拟溃坝洪水波很有效。     

微波辐射相转移催化合成肉桂酸苄酯

采用微波辐射技术结合相转移催化,以肉桂酸和氯化苄为原料在水—氢氧化钾体系中,合成了肉桂酸苄酯。最佳条件为:肉桂酸15.0g,氢氧化钾6.0g,氯化苄16.4mL,PEG-4001.2mL时,微波加热功率400W,反应时间60min,酯收率达90.3%。