Construction and anti-tumor effects of recombinant fowlpox virus expressing Newcastle disease virus hemagglutinin-neuramidinase gene

Hemagglutinin-neuramidinase (HN), a Newcastle disease virus-derived protein, not only mediates receptor recognition but also possesses neuraminidase (NA) activity, the ability to cleave a component of those receptors, N-acetylneuraminic acid (NAcneu, sialic acid). It is known that this protein in mammalian species, including human beings, has interesting anti-neoplastic as well as immune stimulating properties. To explore the use of the HN gene in cancer gene therapy, we constructed a recombi-nant fowlpox virus expressing the HN protein (vFV-HN) and compared the anti-tumor activity of the recombinant virus with that of wild-type fowlpox virus (FPV) in vivo and in vitro. Here we found that although B16 cells were somewhat resistant to the basal cytotoxic effect of wild-type fowlpox virus, infection with vFV-HN caused a pronounced cytotoxic effect and, the survival of tumor-bearing mice immunized with vFV-HN was significantly increased compared with the survival of mice immunized with the FPV alone. Furthermore, the immunization of mice with vFV-HN elicited a B16 tumor-specific cytotoxic T lymphocyte (CTL) response and clonal expansion of both CD4 and CD8 T cell populations in vivo. In addition, T cells from lymph nodes of mice vaccinated with vFV-HN secreted high levels of the Th1 cytokine IL-2 and IFN-γ, indicating that the regression of tumor cells is related to a Th1-type dominant immune response. These results demonstrate that vaccination with vFV-HN may be a potential strategy for cancer gene therapy.     

A new type of triploid crucian crap?red crucian carp (♀)×allotetraploid (♂)

A new type of triploid hybrids (TC) was produced by crossing red crucian carp (♀) with allotetraploid hybrids (♂). This type of triploids with no barbel was spindle-shaped and gray in color. Compared with their parents, some data of the morphological traits in the triploid hybrids were intermediate to those of their parents, and some were beyond their parents with heterozygous traits. Under the same culture conditions, the growth rate of the new type triploids was faster than their maternal red crucian carp (RC). The gonadal development of triploids was obviously slower than that of diploids, and the germ cells in them were degenerated. Compared with another type of triploids produced by Japanese crucian carp (♀) with allotetraploid hybrids (♂), the new type triploids not only kept faster growth rate and sterility, but also had no barbel that was similar to the common carp. The absence of the barbel in the new type of triploids has the important significance in both the inheritance and fish breeding.     

基于查找表的功放线性预失真方法研究

在无线通信系统中,高功率放大器因其具有非线性特性,会导致频谱再生和邻道干扰等问题。为克服这一特征,采用一种基于查找表的自适应预失真方法。首先对查找表进行初始化,再采用自适应算法实时更新查找表内容,从而有效消除功放的非线性失真。仿真实验表明,该自适应预失方法可降低互调失真(IMD),能有效提高功放线性度,从而提高系统性能。     

Subsumption Checking between Concept Queries in Different Ontologies Based on Mutual Instances

This paper proposes a checking method based on mutual instances and discusses three key problems in the method： how to deal with mistakes in the mutual instances and how to deal with too many or too few mutual instances. It provides the checking based on the weighted mutual instances considering fault tolerance, gives a way to partition the large-scale mutual instances, and proposes a process greatly reducing the manual annotation work to get more mutual instances. Intension annotation that improves the checking method is also discussed. The method is practical and effective to check subsumption relations between concept queries in different ontologies based on mutual instances.     

一种适用于教学的酶联免疫吸附试验的抗原抗体系统制备

目的:探讨设计一种教学成本低、便于教学使用、有助于培养医学生综合实验设计能力和提高实验基本操作技能的酶联免疫吸附实验抗原抗体系统.方法:以提纯的人IgG免疫家兔获取兔抗人IgG抗体,再用其包被酶标反应板,以人血清(人IgG)作抗原,用酶标记羊抗人IgG抗体结合抗原及催化底物显色反应.结果:该双抗体夹心法,底物显色反应清楚肉眼即可作出判定,非特异干扰极小.结论:我们设计的酶联免疫吸附实验-双抗体夹心法原理清晰、便于理解和掌握、教学成本较低、结果易于观察、便于教学大量使用.     

一维亚铁磁无序混合自旋链的磁特性的Monte Carlo模拟

基于一维自旋链模型,采用Monte Carlo方法模拟研究了具有反铁磁相互作用的自旋S-1和自旋S-3/2混合的亚铁磁系统的磁特性,重点研究了自旋的无序排列对此混合系统磁特性的影响.研究发现,基态时,当自旋S-1和自旋S-3/2交替混合(有序排列)时,系统存在M-H磁化曲线的阶梯效应;而当自旋S-1和自旋S-3/2无序混合时,系统的阶梯效应出现涨落,台阶甚至消失.最后,通过对系统自旋组态和能量的研究,解释了该混合系统中所产生的阶梯效应和相图.     

昆虫飞行高升力的一种新解释——柔性楔形效应

以探索仿生扑翼飞行机器人研制为目的,在研究"柔性楔形效应"的基础上,将昆翅视为柔性翅,提出用"柔性楔形效应"解释昆虫飞行高升力的观点.指出了用"柔性楔形效应"解释昆虫飞行高升力的仿生学意义.根据该观点,翅翼在自适应变形状态下施以简单的节律运动将是未来仿生扑翼飞行机器人翅翼驱动方式的发展趋势.仿生运动的模拟越简单越容易稳定地实现,实验小组已设计制作了仿生扑翼飞行机器人样机,并成功进行了多次室外放飞.     

二亚苄基山梨糖醇系列产品的合成

研究了合成二亚苄基山梨糖醇(DBS)、二(对甲基苄叉)山梨糖醇(p-CH3-DBS)和二(对氯苄叉)山梨糖醇(p-Cl-DBS)系列产品的投料摩尔比、反应用溶剂、催化剂对甲基苯磺酸和搅拌速度等因素对DBS产品收率和熔点的影响.在苯甲醛(或对甲基苯甲醛、对氯苯甲醛)和山梨糖醇投料摩尔比为(2.0～2.1):1,对甲基苯磺酸、环己烷用量分别为苯甲醛和山梨糖醇投料总质量的4.0%和550%,反应时间6 h条件下,DBS、p-CH3-DBS和p-Cl-DBS产品收率大于70%,熔点分别为210～216 ℃、230～240 ℃和245～260 ℃.     

目前中小企业融资困难的原因及解决措施

目前中小型企业已成为我国经济发展中的重要力量.但中小企业融资难一直是制约其发展的关键因素.其中,既有中小企业自身的问题,也有金融机构、国家政策及传统经济体制方面的影响.我国应充分吸收发达国家的成功经验,通过提高中小企业自身的素质和融资能力,构建全方位、多层次的金融体系,强化政府职能,更多地运用市场手段来解决中小型企业融资问题.     

高产高效综放工作面设备改造研究

论述了成庄矿综放工作面设备改造,包括伸缩机尾改造、顺槽设备的更新与改造和顺槽皮带机的更新。