全文获取类型
收费全文 | 35545篇 |
免费 | 105篇 |
国内免费 | 130篇 |
专业分类
系统科学 | 294篇 |
丛书文集 | 600篇 |
教育与普及 | 87篇 |
理论与方法论 | 96篇 |
现状及发展 | 15316篇 |
研究方法 | 1384篇 |
综合类 | 17465篇 |
自然研究 | 538篇 |
出版年
2013年 | 223篇 |
2012年 | 457篇 |
2011年 | 1001篇 |
2010年 | 192篇 |
2008年 | 580篇 |
2007年 | 668篇 |
2006年 | 678篇 |
2005年 | 660篇 |
2004年 | 632篇 |
2003年 | 652篇 |
2002年 | 553篇 |
2001年 | 1140篇 |
2000年 | 1115篇 |
1999年 | 662篇 |
1994年 | 365篇 |
1992年 | 681篇 |
1991年 | 530篇 |
1990年 | 607篇 |
1989年 | 528篇 |
1988年 | 543篇 |
1987年 | 584篇 |
1986年 | 554篇 |
1985年 | 748篇 |
1984年 | 552篇 |
1983年 | 495篇 |
1982年 | 424篇 |
1981年 | 434篇 |
1980年 | 503篇 |
1979年 | 1141篇 |
1978年 | 921篇 |
1977年 | 863篇 |
1976年 | 725篇 |
1975年 | 804篇 |
1974年 | 985篇 |
1973年 | 874篇 |
1972年 | 865篇 |
1971年 | 1025篇 |
1970年 | 1369篇 |
1969年 | 1045篇 |
1968年 | 976篇 |
1967年 | 963篇 |
1966年 | 893篇 |
1965年 | 659篇 |
1959年 | 383篇 |
1958年 | 609篇 |
1957年 | 431篇 |
1956年 | 393篇 |
1955年 | 359篇 |
1954年 | 379篇 |
1948年 | 281篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Small nuclear ribonucleoprotein polypeptide N (SNRPN), an expressed gene in the Prader-Willi syndrome critical region. 总被引:22,自引:0,他引:22
T Oz?elik S Leff W Robinson T Donlon M Lalande E Sanjines A Schinzel U Francke 《Nature genetics》1992,2(4):265-269
Prader-Willi syndrome (PWS) is associated with paternally derived chromosomal deletions in region 15q11-13 or with maternal disomy for chromosome 15. Therefore, loss of the expressed paternal alleles of maternally imprinted genes must be responsible for the PWS phenotype. We have mapped the gene encoding the small nuclear RNA associated polypeptide SmN (SNRPN) to human chromosome 15q12 and a processed pseudogene SNRPNP1 to chromosome region 6pter-p21. Furthermore, SNRPN was mapped to the minimal deletion interval that is critical for PWS. The fact that the mouse Snrpn gene is maternally imprinted in brain suggests that loss of the paternally derived SNRPN allele may be involved in the PWS phenotype. 相似文献
992.
Dynamin was initially identified in calf brain tissue as a protein of relative molecular mass 100,000 which induced nucleotide-sensitive bundling of microtubules. Purified dynamin showed only trace ATPase activity. But in combination with an activating factor removed during the purification, it exhibited microtubule-activated ATPase activity and dynamin-induced bundles showed evidence of ATP-dependent force production. Dynamin is the product of the Drosophila gene shibire, which has been implicated in synaptic vesicle recycling and, more generally, in the budding of endocytic vesicles from the plasma membrane. Dynamin also shows extensive homology with proteins that participate in vacuolar protein sorting and spindle pole-body separation in yeast, and in interferon-induced viral resistance in mammals. All members of this family contain consensus sequence elements consistent with GTP binding near their amino termini, although none has been shown to have GTPase activity. We report here that dynamin is a specific GTPase which can be stimulated to very high levels of activity by microtubules. 相似文献
993.
Specific low-affinity recognition of major histocompatibility complex plus peptide by soluble T-cell receptor. 总被引:24,自引:0,他引:24
The T-cell receptor is necessary and sufficient for recognition of peptides presented by major histocompatibility complex molecules. Other adhesion molecules, like CD4 or CD8, play an auxiliary role in antigen recognition by T cells. Here we analyse T-cell receptor (TCR) binding using a soluble rather than a cell-bound receptor molecule. A TCR-immunoglobulin chimaera is constructed with the variable and the first constant regions of both the TCR alpha- and beta-chains linked to the immunoglobulin light-chain constant regions. This soluble TCR is expressed, assembled and secreted as an alpha beta heterodimer by a myeloma cell line transfected with the recombinant genes. Furthermore, the soluble TCR is biologically active: it specifically inhibits antigen-dependent activation of the relevant T-cell clones and thus discriminates between proper and irrelevant peptides presented by major histocompatibility complex molecules. 相似文献
994.
995.
Human aminopeptidase N is a receptor for human coronavirus 229E. 总被引:62,自引:0,他引:62
C L Yeager R A Ashmun R K Williams C B Cardellichio L H Shapiro A T Look K V Holmes 《Nature》1992,357(6377):420-422
Human coronaviruses (HCV) in two serogroups represented by HCV-229E and HCV-OC43 are an important cause of upper respiratory tract infections. Here we report that human aminopeptidase N, a cell-surface metalloprotease on intestinal, lung and kidney epithelial cells, is a receptor for human coronavirus strain HCV-229E, but not for HCV-OC43. A monoclonal antibody, RBS, blocked HCV-229E virus infection of human lung fibroblasts, immunoprecipitated aminopeptidase N and inhibited its enzymatic activity. HCV-229E-resistant murine fibroblasts became susceptible after transfection with complementary DNA encoding human aminopeptidase N. By contrast, infection of human cells with HCV-OC43 was not inhibited by antibody RBS and expression of aminopeptidase N did not enhance HCV-OC43 replication in mouse cells. A mutant aminopeptidase lacking the catalytic site of the enzyme did not bind HCV-229E or RBS and did not render murine cells susceptible to HCV-229E infection, suggesting that the virus-binding site may lie at or near the active site of the human aminopeptidase molecule. 相似文献
996.
Retinoic acid alters hindbrain Hox code and induces transformation of rhombomeres 2/3 into a 4/5 identity. 总被引:11,自引:0,他引:11
It has been suggested that Hox genes play an important part in the patterning of limbs, vertebrae and craniofacial structures by providing an ordered molecular system of positional values, termed the Hox code. Little is known about the nature of the signals that govern the establishment and regulation of Hox genes, but retinoic acid can affect the expression of these genes in cell lines and in embryonic tissues. On the basis of experimental and clinical evidence, the hindbrain and branchial region of the head are particularly sensitive to the effects of retinoic acid but the phenotypes are complex and hard to interpret, and how and if they relate to Hox expression has not been clear. Here we follow the changes induced by retinoic acid to hindbrain segmentation and the branchial arches using transgenic mice which contain lacZ reporter genes that reveal the endogenous segment-restricted expression of the Hox-B1 (Hox-2.9), Hox-B2(Hox-2.8) and Krox-20 genes. Our results show that these genes rapidly respond to exposure to retinoic acid at preheadfold stages and undergo a progressive series of changes in segmental expression that are associated with specific phenotypes in hindbrain of first branchial arch. Together the molecular and anatomical alterations indicate that retinoic acid has induced changes in the hindbrain Hox code which result in the homeotic transformation of rhombomeres (r) 2/3 to an r4/5 identity. A main feature of this rhombomeric phenotype is that the trigeminal motor nerve is transformed to a facial identity. Furthermore, in support of this change in rhombomeric identity, neural crest cells derived from r2/3 also express posterior Hox markers suggesting that the retinoic acid-induced transformation extends to multiple components of the first branchial arch. 相似文献
997.
Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. 总被引:118,自引:0,他引:118
998.
999.
Effects of an Rb mutation in the mouse. 总被引:126,自引:0,他引:126
The retinoblastoma gene is mutated in several types of human cancer and is the best characterized of the tumour-suppressor genes. A mouse strain has been constructed in which one allele of Rb is disrupted. These heterozygous animals are not predisposed to retinoblastoma, but some display pituitary tumours arising from cells in which the wild-type Rb allele is absent. Embryos homozygous for the mutation die between days 14 and 15 of gestation, exhibiting neuronal cell death and defective erythropoiesis. 相似文献
1000.