首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   32682篇
  免费   151篇
  国内免费   305篇
系统科学   456篇
丛书文集   495篇
教育与普及   109篇
理论与方法论   99篇
现状及发展   12715篇
研究方法   1306篇
综合类   17279篇
自然研究   679篇
  2013年   413篇
  2012年   675篇
  2011年   1353篇
  2010年   455篇
  2009年   442篇
  2008年   793篇
  2007年   911篇
  2006年   917篇
  2005年   845篇
  2004年   657篇
  2003年   607篇
  2002年   575篇
  2001年   1016篇
  2000年   1039篇
  1999年   693篇
  1994年   342篇
  1992年   583篇
  1991年   466篇
  1990年   516篇
  1989年   468篇
  1988年   430篇
  1987年   480篇
  1986年   469篇
  1985年   602篇
  1984年   468篇
  1983年   382篇
  1982年   384篇
  1981年   350篇
  1980年   390篇
  1979年   958篇
  1978年   705篇
  1977年   713篇
  1976年   575篇
  1975年   619篇
  1974年   802篇
  1973年   707篇
  1972年   671篇
  1971年   770篇
  1970年   999篇
  1969年   797篇
  1968年   795篇
  1967年   788篇
  1966年   720篇
  1965年   544篇
  1959年   274篇
  1958年   455篇
  1957年   317篇
  1956年   283篇
  1955年   284篇
  1954年   262篇
排序方式: 共有10000条查询结果,搜索用时 78 毫秒
71.
报道一种新颖的激光开槽、埋槽电极硅太阳电池的结构、工艺流程及其研制结果。在AM1.5,25℃,100mW/cm2的条件下,以面积为45cm3的36片硅片研制的硅太阳电池的输出参数的平均值为JSC=36.1mA/cm2,V∞=633mV,F.F.=0.798,η=18.23%,最后分析了种高性能硅太阳电池的设计特点。  相似文献   
72.
Low-molecular-weight GTP-binding proteins are strong candidates for regulators of membrane traffic. In yeast, mutations in the sec4 or ypt1 genes encoding small GTP-binding proteins inhibit constitutive membrane flow at the plasma membrane or Golgi complex, respectively. It has been suggested that membrane fusion-fission events are regulated by cycling of small GTP-binding proteins between a membrane-bound and free state, but although most of these small proteins are found in both soluble and tightly membrane-bound forms, there is no direct evidence to support such cycling. In rat brain a small GTP-binding protein, rab3A, is exclusively associated with synaptic vesicles, the secretory organelles of nerve terminals. Here we use isolated nerve terminals to study the fate of rab3A during synaptic vesicle exocytosis. We find that rab3A dissociates quantitatively from the vesicle membrane after Ca2(+)-dependent exocytosis and that this dissociation is partially reversible during recovery after stimulation. These results are direct evidence for an association-dissociation cycle of a small GTP-binding protein during traffic of its host membrane.  相似文献   
73.
对目前的UBBE模型进行了适当的推广,使在解决估计问题时可以考虑那些可能是误差上界的数值,并在此基础上提出了一种方法,能够通过对估计精度和可靠性进行合理的权衡确定所需估计值,实际案例研究结果表明所提方法能够较好地解决估计精度和可靠性之间的矛盾。  相似文献   
74.
We develop in this paper an efficient way to select the best subset threshold autoregressive model. The proposed method uses a stochastic search idea. Differing from most conventional approaches, our method does not require us to fix the delay or the threshold parameters in advance. By adopting the Markov chain Monte Carlo techniques, we can identify the best subset model from a very large of number of possible models, and at the same time estimate the unknown parameters. A simulation experiment shows that the method is very effective. In its application to the US unemployment rate, the stochastic search method successfully selects lag one as the time delay and five best models from more than 4000 choices. Copyright © 2003 John Wiley & Sons, Ltd.  相似文献   
75.
提出了一种基于IP多播技术的解决方案并加以实现,满足了对上机学生按系或班级分组进行管理与各类统计的需要,实现了传统C/S结构所能实现的全部功能,解决了传统机房信息管理系统使用带来的问题。  相似文献   
76.
Analysis of 78 Huntington's disease (HD) chromosomes with multi-allele markers revealed 26 different haplotypes, suggesting a variety of independent HD mutations. The most frequent haplotype, accounting for about one third of disease chromosomes, suggests that the disease gene is between D4S182 and D4S180. However, the paucity of an expected class of chromosomes that can be related to this major haplotype by assuming single crossovers may reflect the operation of other mechanisms in creating haplotype diversity. Some of these mechanisms sustain alternative scenarios that do not require a multiple mutational origin for HD and/or its positioning between D4S182 and D4S180.  相似文献   
77.
T lymphocytes recognize antigen in the form of peptides that associate with specific alleles of class I or class II major histocompatibility (MHC) molecules. By contrast with the clear MHC allele-specific binding of peptides to purified class II molecules purified solubilized class I molecules either bind relatively poorly or show degenerate specificity. Using photo-affinity labelling, we demonstrate here the specific interaction of peptides with cell-associated MHC class I molecules and show that this involves metabolically active processes.  相似文献   
78.
J M Nunnari  D L Zimmerman  S C Ogg  P Walter 《Nature》1991,352(6336):638-640
The rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites. A purification to apparent homogeneity of a negatively charged protein (ERp180) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERp180. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.  相似文献   
79.
D C Gautam  L Kapoor 《Experientia》1991,47(3):280-282
Genotoxic effects of dithane M-45 were studied on the bone marrow cells of male albino mice (Lacca strain) in vivo. Different doses (30 mg, 40 mg and 300 mg/kg b.wt) of dithane M-45 were injected intraperitoneally and their effects were investigated after time intervals of 1, 2, 5 and 10 days. The chromosomal aberrations observed in the bone marrow cells of male mice after treatment with dithane M-45 were fragments, rings, dicentric chromosomes, terminal chromatid deletions, chromatid gaps and breaks. In addition to these chromosomal aberrations, physiological effects such as uneven stretching of chromatin material, end-to-end chromosomal associations, exchange configurations, clumping, stickiness and centromeric associations were also observed.  相似文献   
80.
秋水仙素诱导青虾次级卵母细胞二倍体初探   总被引:3,自引:0,他引:3  
以青虾卵巢为材料,采用秋水仙素诱导多倍体次级卵母细胞.经滴片试验,确立此实验最佳滴片高度为1.5cm,通过设置5个秋水仙素浓度(0.08~0.40mg/mL)及3个作用时间(6~24h),观察发生染色体加倍的细胞相对数目.实验结果表明:在使用秋水仙素的浓度为0.08~0.40mg/mL,处理6~24h时都可以获得多倍体卵细胞.在秋水仙素浓度是0.32mg/mL,处理6h可得到最高二倍体突变率(32.58%).还测试了0.32mg/mL COM作用6h时ATPase活性与生理盐水对照组的变化,结果显示COM会在一定程度上影响细胞的新陈代谢.但是50%左右的成活率表明,这种诱导突变的方法仍具有一定的应用价值.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号