The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X

We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.     

Systematic variation in gene expression patterns in human cancer cell lines

We used cDNA microarrays to explore the variation in expression of approximately 8,000 unique genes among the 60 cell lines used in the National Cancer Institute's screen for anti-cancer drugs. Classification of the cell lines based solely on the observed patterns of gene expression revealed a correspondence to the ostensible origins of the tumours from which the cell lines were derived. The consistent relationship between the gene expression patterns and the tissue of origin allowed us to recognize outliers whose previous classification appeared incorrect. Specific features of the gene expression patterns appeared to be related to physiological properties of the cell lines, such as their doubling time in culture, drug metabolism or the interferon response. Comparison of gene expression patterns in the cell lines to those observed in normal breast tissue or in breast tumour specimens revealed features of the expression patterns in the tumours that had recognizable counterparts in specific cell lines, reflecting the tumour, stromal and inflammatory components of the tumour tissue. These results provided a novel molecular characterization of this important group of human cell lines and their relationships to tumours in vivo.     

简论机车车辆的可靠性与维修性及维修信息管理

论述了机车车辆"以可靠性为中心的维修"理论中的可靠性和维修性的定义、评价指标和设计方法,讨论了两者之间的关系;并对维修信息管理作了分析,说明了在高速、重载铁路迅速发展的今天,优良的维修管理信息系统对越来越复杂的机车车辆维修具有重要意义.     

ON THE CONSTRUCTION OF ALGEBRAIC-GEOMETRIC CODES

1,Illtroduction'SincetheearlypapersbyG0ppa[1-31,algebralc-ge0metriccodeshavebeeninthespotlight0fcodingthe0reticresearchf0rab0uttwodecades.NumerousexcitingresultshavebeenachievedusingGoppa'sconstructi0noflinearcodesfromalgebralccurvesoverfinitefields,bothbyalgebraicge0metorsandcodingtheorists.Becauseofthedifficultyofthesubject,severalexplanatorypaPersandtextbookshaveappeared,seeforinstance[4-8].Inthispaperwepresentaquitedtherentpointofviewconcerningalgebraic-geometriccodes.Itiswellknownthataq…     

浅谈水利施工项目的成本控制

水利工程施工项目成本是指水利施工企业为完成施工项目的全部工程任务所耗费的各项生产费用的总和.对施工项目进行成本控制是施工项目管理工作的中心内容,贯穿于项目管理活动的全过程.     

复杂环境下基于动态贝叶斯网络的目标识别

为了提升高动态复杂电磁环境下空战过程中对目标的识别能力,针对SBN网络模型无法满足战场的动态性要求以及对目标的经常性误识别问题,设计了一种基于变结构动态贝叶斯网络的目标类型识别模型。该模型是由静态贝叶斯网络模型演变而来,具有良好的动态表达性和滤波功能,弥补了SBN的不足,并且对空战过程中目标特征信息丢失的问题有良好的容错能力。仿真结果表明,基于动态贝叶斯网络的目标识别的识别效果,优于基于参数学习贝叶斯网络的目标识别。使用该模型后目标识别的准确性提高了5%,有效地解决目标类型识别过程中数据缺失和信息不足的问题。     

应用最优化：工程系统的表述与算法

将一个问题用数值表示其出发点是抓住问题本身的直观思想并且转换为恰当的数学语言。本书采用逐步深入的方法刻划如何表述数学问题，通过这种描述人们可以应用现有的软件去解决它。作者根据数值问题的不同类型论述了求解的技术，通过许多来自工程技术领域的典型实例详细地解说了问题由直观叙述到易于控制的数值形式的转换过程，特别包含了许多有效算法。