Temperature-induced hand inversion inBacillus subtilis macrofibers

Cellular and Molecular Life Sciences -     

Hybridization histochemistry

Summary The location of gene expression by hybridization histochemistry is being applied in many areas of research and diagnosis. The aim of this technique is to detect specific mRNA in cells and tissues by hybridization with a complementary DNA or RNA probe. Requirements for optimal specificity, sensitivity, resolution and speed of detection may not all be encompassed in one simple technique suitable for all applications, thus appropriate procedures should be selected for specific objectives. With reference to published procedures and our own extensive experience, we have evaluated fixatives, probes, labels and other aspects of the technique critical to the preservation and hybridization in situ of mRNA and detection and quantition of hybrids.     

A practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity

Summary A known ornithine decarboxylase assay working with ion exchang separation of [³H]ornithine and [³H]putrescine has been revised. The assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. The separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. The detection limit is about 50 pmoles [³H]putrescine formed.     

Extracellular localization of a protein antigen in lymphatic tissues in contact with lymphocytes

Zusammenfassung Drei Stunden nach i.v.-Gabe an Kaninchen ist das Antigen Meerrettich Peroxydase extrazellulär in den Marksträngen der Lymphknoten und der roten Pulpa der Milz elektronenmikroskopisch nachweisbar. Das unveränderte Antigen steht damit noch zu dieser Zeit in direktem Kontakt mit der Zellmembran der lymphoiden Zellen.     

Chemical constituents of the antibiotic fraction ofArnebia nobilis

Zusammenfassung Aus den Wurzeln vonArnebia nobilis wurden 4 antibiotisch wirksame Stoffe isoliert. Die Struktur von dreien wurde aufgeklärt.

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On deputation from the Chemistry Department, Lucknow University.

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To whom enquiries should be made. The isolation of shikonin monoacetate and,-dimethylacrylate fromLithosperm erythrorhizon, I.Morimoto, T.Kishi, S.Ikegana and Y.Hirata, Tetrahedron Lett.4737 (1965), has been brought to our notice since communicating this paper.

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Communication No. 1305 from the Central Drug Research Institute.     

The role of the diffusible factor released by the egg jelly in fertilization of the toad egg

Resumen Los experimentes descritos indican que el factor difusible de las cubiertas gelatinosas de los ovocitos deBufo arenarum activaría a los espermatozoides a través de un efecto alcalinizante sobre el medio de inseminación.