全文获取类型
收费全文 | 244篇 |
免费 | 2篇 |
国内免费 | 5篇 |
专业分类
系统科学 | 11篇 |
教育与普及 | 1篇 |
理论与方法论 | 1篇 |
现状及发展 | 37篇 |
研究方法 | 31篇 |
综合类 | 150篇 |
自然研究 | 20篇 |
出版年
2021年 | 1篇 |
2020年 | 2篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2014年 | 6篇 |
2013年 | 5篇 |
2012年 | 14篇 |
2011年 | 39篇 |
2010年 | 3篇 |
2008年 | 14篇 |
2007年 | 20篇 |
2006年 | 17篇 |
2005年 | 31篇 |
2004年 | 18篇 |
2003年 | 16篇 |
2002年 | 19篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 3篇 |
1984年 | 2篇 |
1979年 | 2篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1969年 | 2篇 |
1965年 | 1篇 |
1947年 | 1篇 |
1946年 | 1篇 |
1945年 | 1篇 |
排序方式: 共有251条查询结果,搜索用时 0 毫秒
101.
Irigoien X Harris RP Verheye HM Joly P Runge J Starr M Pond D Campbell R Shreeve R Ward P Smith AN Dam HG Peterson W Tirelli V Koski M Smith T Harbour D Davidson R 《Nature》2002,419(6905):387-389
Diatoms dominate spring bloom phytoplankton assemblages in temperate waters and coastal upwelling regions of the global ocean. Copepods usually dominate the zooplankton in these regions and are the prey of many larval fish species. Recent laboratory studies suggest that diatoms may have a deleterious effect on the success of copepod egg hatching. These findings challenge the classical view of marine food-web energy flow from diatoms to fish by means of copepods. Egg mortality is an important factor in copepod population dynamics, thus, if diatoms have a deleterious in situ effect, paradoxically, high diatom abundance could limit secondary production. Therefore, the current understanding of energy transfer from primary production to fisheries in some of the most productive and economically important marine ecosystems may be seriously flawed. Here we present in situ estimates of copepod egg hatching success from twelve globally distributed areas, where diatoms dominate the phytoplankton assemblage. We did not observe a negative relationship between copepod egg hatching success and either diatom biomass or dominance in the microplankton in any of these regions. The classical model for diatom-dominated system remains valid. 相似文献
102.
Sexual mimicry among animals is widespread, but does it impart a fertilization advantage in the widely accepted 'sneak-guard' model of sperm competition? Here we describe field results in which a dramatic facultative switch in sexual phenotype by sneaker-male cuttlefish leads to immediate fertilization success, even in the presence of the consort male. These results are surprising, given the high rate at which females reject copulation attempts by males, the strong mate-guarding behaviour of consort males, and the high level of sperm competition in this complex mating system. 相似文献
103.
Aminoglycoside antibiotics induce bacterial biofilm formation 总被引:2,自引:0,他引:2
Biofilms are adherent aggregates of bacterial cells that form on biotic and abiotic surfaces, including human tissues. Biofilms resist antibiotic treatment and contribute to bacterial persistence in chronic infections. Hence, the elucidation of the mechanisms by which biofilms are formed may assist in the treatment of chronic infections, such as Pseudomonas aeruginosa in the airways of patients with cystic fibrosis. Here we show that subinhibitory concentrations of aminoglycoside antibiotics induce biofilm formation in P. aeruginosa and Escherichia coli. In P. aeruginosa, a gene, which we designated aminoglycoside response regulator (arr), was essential for this induction and contributed to biofilm-specific aminoglycoside resistance. The arr gene is predicted to encode an inner-membrane phosphodiesterase whose substrate is cyclic di-guanosine monophosphate (c-di-GMP)-a bacterial second messenger that regulates cell surface adhesiveness. We found that membranes from arr mutants had diminished c-di-GMP phosphodiesterase activity, and P. aeruginosa cells with a mutation changing a predicted catalytic residue of Arr were defective in their biofilm response to tobramycin. Furthermore, tobramycin-inducible biofilm formation was inhibited by exogenous GTP, which is known to inhibit c-di-GMP phosphodiesterase activity. Our results demonstrate that biofilm formation can be a specific, defensive reaction to the presence of antibiotics, and indicate that the molecular basis of this response includes alterations in the level of c-di-GMP. 相似文献
104.
Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis 总被引:1,自引:0,他引:1
Glasson SS Askew R Sheppard B Carito B Blanchet T Ma HL Flannery CR Peluso D Kanki K Yang Z Majumdar MK Morris EA 《Nature》2005,434(7033):644-648
Human osteoarthritis is a progressive disease of the joints characterized by degradation of articular cartilage. Although disease initiation may be multifactorial, the cartilage destruction appears to be a result of uncontrolled proteolytic extracellular matrix destruction. A major component of the cartilage extracellular matrix is aggrecan, a proteoglycan that imparts compressive resistance to the tissue. Aggrecan is cleaved at a specific 'aggrecanase' site in human osteoarthritic cartilage; this cleavage can be performed by several members of ADAMTS family of metalloproteases. The relative contribution of individual ADAMTS proteases to cartilage destruction during osteoarthritis has not been resolved. Here we describe experiments with a genetically modified mouse in which the catalytic domain of ADAMTS5 (aggrecanase-2) was deleted. After surgically induced joint instability, there was significant reduction in the severity of cartilage destruction in the ADAMTS5 knockout mice compared with wild-type mice. This is the first report of a single gene deletion capable of abrogating the course of cartilage destruction in an animal model of osteoarthritis. These results demonstrate that ADAMTS5 is the primary 'aggrecanase' responsible for aggrecan degradation in a murine model of osteoarthritis, and suggest rational strategies for therapeutic intervention in osteoarthritis. 相似文献
105.
Tomita S Adesnik H Sekiguchi M Zhang W Wada K Howe JR Nicoll RA Bredt DS 《Nature》2005,435(7045):1052-1058
AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) receptors mediate fast excitatory synaptic transmission in the brain. These ion channels rapidly deactivate and desensitize, which determine the time course of synaptic transmission. Here, we find that the AMPA receptor interacting protein, stargazin, not only mediates AMPA receptor trafficking but also shapes synaptic responses by slowing channel deactivation and desensitization. The cytoplasmic tail of stargazin determines receptor trafficking, whereas the ectodomain controls channel properties. Stargazin alters AMPA receptor kinetics by increasing the rate of channel opening. Disrupting the interaction of stargazin ectodomain with hippocampal AMPA receptors alters the amplitude and shape of synaptic responses, establishing a crucial function for stargazin in controlling the efficacy of synaptic transmission in the brain. 相似文献
106.
C. Regnault Roger 《Cellular and molecular life sciences : CMLS》1988,44(9):725-733
Summary Examination of the physiological activity of flavonoids in relation to their antiscorbutic properties shows that some of these compounds, the flavan-3=ols, have a particular nutritional impact and consequently should be distinguished from the rest of the flavonoids and polyphenolds. Therefore, the use of the term Vitamin P and Bioflavonoids is also discussed. 相似文献
107.
研究柠条深度裂解产生的生物炭作为无土栽培机制的可行性,以探讨柠条的综合开发和利用.试验分析了生物炭和草炭复配基质的理化特性,并对小白菜做育苗期盆栽试验.结果表明:当草炭和柠条炭的质量比为4∶2时达到最佳配比,此时混配基质各项指标均符合理想基质的要求;柠条炭具有强大的吸附能力和丰富的孔隙结构,其作为草炭的混配基质能够较大程度提高基质的透气性和速效钾含量,有贮存和缓释养分的作用. 相似文献
108.
This paper explores some of the unilluminated or less explicit aspects of issues faced by those using systems approaches in real-world problem situations. The paper contends that discourse on systems approaches tends to be dominated by rational logical aspects of methodology, though other aspects of the intervention process, such as political dimensions of legitimacy, have become more important in the last 10–15 years. Unsurprisingly the discourse has been different for different methods. Method has been largely invisible in the hard systems area (or at least relatively little discussed), and although it has been the subject of much debate in the soft systems area, the debate has been nonetheless narrowly defined. It is narrowly defined in its relative neglect of process aspects for conducting a systems study. Since the nature of these processes (for example, the client/consultant relation) changes from hard systems to soft systems to critical systems in a way that makes social process progressively a more important dimension of each approach, this aspect has featured more in discourse on critical systems, but in general it remains a curious area of neglect. There is increasing interest in this area, and some signs that relevant theory and practice from closely related domains is being accessed to strengthen these approaches. This paper attempts to make a contribution by outlining and discussing some areas that could usefully complement existing systems approaches. The paper considers the following areas: (a) client relations, (b) analyst role, (c) language and communication, (d) group processes, (e) culture (and rationalities), (f) information gathering techniques and processes, and (g) change management or implementation. The paper draws on experiences of systems practices in the literature, interviews with systems practitioners, and writings in related areas. The paper ends by discussing some of the implications of these issues for the development of well-rounded systems approaches. 相似文献
109.
Trond Roger Oskars Constantine Mifsud Manuel António E. Malaquias 《Journal of Natural History》2017,51(27-28):1593-1608
Atys jeffreysi is a heterobranch Cephalaspidea gastropod belonging to the family Haminoeidae occurring in the Mediterranean Sea, Madeira and Canary archipelagos. Nearly nothing is known about the internal anatomical features of this species. In this paper we redescribe the species A. jeffreysi based on fine anatomical work and scanning electron microscopy. DNA barcodes are provided for the first time for A. jeffreysi and A. macandrewii, the only two species of the genus native in the Mediterranean Sea. The genetic distance (COI uncorrected p-distance) between them is estimate at 21.6%. A Bayesian molecular phylogeny based on the gene marker cytochrome c oxidase subunit I (COI) including all sequences available of the genera Aliculastrum, Liloa and Atys species did not support a sister relationship between the two Mediterranean species and suggests that they are more closely related to distinct Indo-West Pacific lineages. The complex systematics of the genus Atys is briefly discussed. 相似文献
110.
Accurate time- and cost-efficient mapping is central to successful rangeland invasive plant management. In this study sampling together with Inverse Distance Weighted (IDW) interpolation modeling was tested as a mapping alternative to expensive full-coverage delineation survey mapping methods. Our objective was to examine accuracies of presence/absence maps generated from 18 sampling strategies (3 sampling methods × 6 sample densities) using IDW. Invasive plant field survey maps with known accuracies were used to generate samples and to test interpolation results at 2 sites. Site 1 was approximately 6.0 km 2 , dominated by Russian knapweed ( Acroptilon repens L.). Site 2, an upland area of approximately 13.5 km 2 , was dominated by spotted knapweed ( Centaurea maculosa Lam). Sampling method × sample density combinations were gathered from field survey infestation maps using repeated computer-based sampling methods. IDW modeling was applied to each of the sample data sets. Accuracies of the IDW interpolation results were calculated by re-referencing field survey maps. We determined that sampling at density of 0.25% (about 1 point per ha) using a systematic sampling method was the preferred sampling strategy for both sites. This combination of sampling density and method yielded 85% accurate presence/absence maps. We conclude that sampling combined with IDW interpolation modeling can generate accurate invasive plant maps and is a potential alternative to current delineation survey methods. 相似文献