Dissociation of glucocorticoid effects of C-21 steroids at high concentrations in thymocytes

Summary A dissociation between inhibition of RNA synthesis and cell lysis was observed when thymocytes of adrenalectomized rats were incubated with high concentrations of pregn-4-ene-11-ol-3, 20-dione and pregna-1,4-diene-11-ol-3,20-dione. In contrast, no dissociation of these effects was found with the typical glucocorticoids cortisol and corticosterone, nor with their 1,4-diene analogs under the same conditions.Acknowledgments. This work was supported by grants from the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina to the Instituto de Biologia y Medicina Experimental and the Programa de Regulación Hormonal y Metabólica as well as by financial help from the Secretaría de Ciencia y Tecnología.     

The ocean as a supplier of food and energy

Summary This paper presents the concept of raising seaweeds and other valuable organisms with the aid of huge structures (ocean farms) emplaced in the surface waters of the open oceans. Potential advantages from and difficulties to be expected in realizing the associated technologies are briefly set forth. Much of the published literature pertaining to the concept is referenced and summarized. Wave-powered upwelling of cool, nutrient-rich waters through vertical pipes extending to depths of 100–300 m is indicated as desirable. Technologies are outlined for using the harvested seaweeds to create foods and other valuable products such as animal feeds, fertilizers, fibers, plastics, synthetic natural gas (methane), and alcohol and gasoline fuels. Results from site selection studies and economic analyses are given. It appears that dynamically positioned farms orbiting with the surface current patterns typically found on the ocean will be most cost-effective. The general conclusion is stated that open ocean farming will become economically more feasible as the cheaper fossil fuels and food producing lands of the earth become increasingly consumed in the course of the next century.     

Identification of an acyclic diterpene alcohol in the defense secretion of soldiers ofReticulitermes lucifugus

Summary The defense secretion of soldiers ofReticulitermes lucifugus has been shown to contain, predominantly, (R)-(–)-(E,E)-geranyllinalool together with germacrene A and -farnesene.This report covers part of a collaborative study with J.-L. Clément, Lab. d'Evolution, Université P. et M. Curie, Paris, to whom we are grateful for supplies of material and discussions. We also thank Dr O.T. Jones for collections ofReticulitermes lucifugus.     

Genetic polymorphisms and intrauterine development. Evidence of decreased heterozygosity in light-for-dates human newborn babies

Summary In 2 independent samples of low-birth-weight infants the proportion of females and homozygotes for a series of polymorphic systems was higher in light-for-dates than in preterm babies. The observation seems to give support to the hypothesis that homozygosity for normal polymorphisms may decrease in general intrauterine growth rate. Since it is known that survival rate is strongly related to birth weight, a correlation between growth retardation and homozygosity may have a major role in the maintenance of such polimorphisms.We thank Prof. L. Ginzburg for helpful comments.     

Birefringence signals and tension development in single frog muscle fibres at short stimulus intervals

Summary The early large birefringence signal and mechanical activity were studied together in isolated single fibres of frog skeletal muscle with double stimulation at short stimulus intervals (2–60 msec) at room temperature and at 4–6°C. In all fibres tested, extra tension and additional birefringence signal in response to the second stimulus appeared simultaneously and suddenly upon increasing the stimulus interval. The shape of the stimulus-interval versus tension-development curve makes it highly improbable that subthreshold calcium release occurs at shorter stimulus intervals; therefore, tension development reliably reflects Ca-release in these experiments. In contrast to the report by Suarez-Kurtz and Parker, birefringence signal and calcium release are shown not to be dissociated by double stimulation. This result supports the hypothesis that the early large birefringence signal is an intrinsic indicator of calcium release from the sr during EC-coupling in skeletal muscle.     

Experimental desquamation of intestinal epithelium for in vivo studies of regeneration

Summary Complete removal of villous as well as upper-crypt epithelium was achieved in vivo by vibration of an intestinal segment using tetraphenylboron sodium (TPB) as a disassociation agent.Acknowledgments. The authors thank Hicol Industrieterrein De Bosschen for supplying TPB. Research was supported by MR-II 1.3.14.p.2 grant.     

Plasma growth hormone in the rabbit fetus. Relation to maturation of the liver and lung]

Day 25 after insemination is a date of peculiar importance in the maturation of several organs in the Rabbit fetus. From day 25 onward the fetal liver stores increasing amounts of glycogen and the lung stores increasing amounts of lecithins, concomitant with sudden rise in the activity of lung phosphatidic-acid phosphohydrolase. Earlier studies on decapitated fetuses established that glycogen storage in the liver is dependent on a dual hormonal control, comprising a pituitary hormone like growth hormone or prolactin (some placental hormones share the same activity) and corticosteroids (Jost, 1961). Since the variations in endogenous corticosteroids do not seem to herald these liver or lung changes (Mulay et al., 1973), a study was made of growth hormone. Plasma immunoreactive growth hormone--determined with a heterologous Rat system (Kervran et al., 1976)--increases eightfold between days 23 and 25. During the same time plasma prolactin does not change according to McNeily and Friesen, 1978, and to unpublished data obtained with Dr McNeilly. In preliminary assays, Rat growth hormone was seen to increase phosphorylase "a" activity in the lung of 18.5 day-old Rat fetuses, thus anticipating normal development. We suggest that growth hormone plays a role in initiating liver and lung maturation.     

Genetic control by the HLA region of the immune response to factor VIII in hemophilic patients]

In 10 sibships of haemophilic A patients, every one including 1 haemophilic brother with an antibody to factor VIII and 1 haemophilic brother without antibody, pattern of HLA segregation was analysed. All sibships were HLA haploidentical or different and not HLA identical. This fact suggested a linkage between the major histocompatibility complex and an "Ir" gene to factor VIII.     

Field bioassay of male Douglas-fir beetle compound 3-methylcyclohex-3-en-1-one

Summary Synthetic 3-methylcyclohex-3-en-1-one, or 3,3-MCH, decreased by 77% the flight aggregation ofDendroctonus pseudotsugae (Scolytidae) to sticky traps containing attractant (frontalin, -pinene, and resin). However, windthrown trees were protected by 3,3-MCH much less than is known to occur with the isomer 3,2-MCH.This research was supported in part by NSF grants BMS 72-02522 and DEB 77-11361 and the O.S.U. School of Forestry. We thank the administration of the Siuslaw National Forest for study sites, and Dr L.M. Libbey for GC/MS of 3,3-MCH. Oregon State University Ag. Exp. Sta. Tech. Paper No. 4313.