DNA-polyintercalation compounds. Synthesis of ellipticine dimers]

Many symmetrical bis [9-methoxy 2-ellipticinium] dioxodiazaalkanes salts were synthesized from 9-methoxy ellipticine. An analog derivative of 9-hydroxy ellipticine was also prepared. Some of these dimers bind to DNA by a bifunctionnal mode of intercalation.     

Vesicles carrying nuclear material in matureCyprinus carpio erythrocytes

Zusammenfassung Chromatinhaltige feulgenpositive Bläschen wurden im Cytoplasma der Erythrocyten vonCyprinus carpio gefunden. Sie haben ihren Ursprung in den unmittelbar an der Kernmembran anliegenden Mitochondrien, die während der Chromatinaufnahme allmählich ihre Struktur verlieren.

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The authors wish to thank Mrs.Vera Mondin Weisz and Mr.C. A. Gonçalves Silva for their technical assistance and Mrs.Sibylle Heller for her editorial aid and translation. This research has been supported by the Conselho Nacional de Pesquisas (Proc. No. 10112/71) and Fundo Especial de Despesas do Instituto Butantan.     

Spectral changes resulting from the interaction of some N-alkyl nitrosamines and rat liver microsomes

Summary Dimethyl (DMN) and diethyl nitrosamine (DEN) do not give characteristic spectral changes upon interaction with rat liver microsomes, while dipropyl (DPN) and dibutyl (DBN) nitrosamine cause type I spectral changes. The spectral binding constant is 100 mM for DPN and 1.17 mM for DBN. The maximal spectral change is 3.2×10⁶ and 1.0×10⁶ absorbance units per milligram protein for DPN and DBN respectively.Acknowledgment. This work was supported by Grants AM 13195-07 from the National Institute of Health (USA) and from the Consejo Nacional de Investigaciones Cientificas y Técnicas (Argentina).     

EEG synchronization produced by peripheral nerve stimulation

Riassunto La stimolazione a 3–8/sec di nervi cutanei e muscolari, eseguita in gatti integri non anestetizzati, produce una sincronizzazione dell'attività elettrica cerebrale. La soglia per questa risposta corrisponde in media a 0,4–0,9 volte l'intensità minima di corrente che applicata allo stesso nervo e in analoghe condizioni sperimentali produce la reazione di risveglio.

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This investigation was supported by PHS research grant B-2990 from the National Institute of Neurological Diseases and Blindness, N.I.H., Public Health Services, U.S.A.