萃取浮选光度法测定微量钼的研究及应用

研究了用甲苯萃取浮选Mo(Ⅴ)—SCN~-—Kφ三元络合物的各种条件。在硫酸介质中,紫红色络合物最大吸收波长位于585nm,摩尔吸光系数为2.8×10~5l·mol~(-1)·cm~(-1),钼量在0—10μg/25ml范围内符合比耳定律。大量常见元素均不干扰,40倍的钨(Ⅵ)亦不干扰钼的测定,方法已应用于钨矿中微量钼的测定,其结果令人满意。     

Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607

Several hundred million tons of toxic mercurials are dispersed in the biosphere. Microbes can detoxify organo-mercurials and mercury salts through sequential action of two enzymes, organomercury lyase and mercuric ion reductase (MerA). The latter, a homodimer with homology to the FAD-dependent disulphide oxidoreductases, catalyses the reaction NADPH + Hg(II)----NADP+ + H+ + Hg(0), one of the very rare enzymic reactions with metal substrates. Human glutathione reductase serves as a reference molecule for FAD-dependent disulphide reductases and between its primary structure and that of MerA from Tn501 (Pseudomonas), Tn21 (Shigella), p1258 (Staphylococcus) and Bacillus, 25-30% of the residues have been conserved. All MerAs have a C-terminal extension about 15 residues long but have very varied N termini. Although the enzyme from Streptomyces lividans has no addition, from Pseudomonas aeruginosa Tn501 and Bacillus sp. strain RC607 it has one and two copies respectively of a domain of 80-85 residues, highly homologous to MerP, the periplasmic component of proteins encoded by the mer operon. These domains can be proteolytically cleaved off without changing the catalytic efficiency. We report here the crystal structure of MerA from the Gram-positive bacterium Bacillus sp. strain RC607. Analysis of its complexes with nicotinamide dinucleotide substrates and the inhibitor Cd(II) reveals how limited structural changes enable an enzyme to accept as substrate what used to be a dangerous inhibitor. Knowledge of the mode of mercury ligation is a prerequisite for understanding this unique detoxification mechanism.     

适应市场需求积极推进吕梁农业结构高速调整

文章结合吕梁地区实际，对如何适应市场需求确立农村经济结构调整方向和进行产业化经营进行了论证分析。     

谈旧桥加固和桥梁病害处理技术

根据山西省现有公路和桥梁状况，为恢复和提高旧桥的承载能力及通过能力，延长桥梁使用寿命，迫切需要进行评价和加固改造。     

大学《现代教育技术》课程教学中探究式学习模式的应用与思考

本文从探究式学习的定义和特征出发,反对不分教学对象差异的"唯探究主义",在大学《现代教育技术》课程的教学中倡导面向全体学生,开展多样化的探究式学习。这就要求在教学过程中,辩证地处理学生自主与教师指导的关系,强调教师与学生的相互倾听,珍视探究中学生的个人观念、独特感受和体验,并引导学生积极反思。     

模糊Riemann-Stieltjes积分的收敛定理

模糊Riemann-Stieltjes积分是模糊分析中的一类重要的模糊积分,但相应的积分序列的收敛定理尚未见到.将给出模糊数值函数列关于实函数和模糊数值函数关于实函数列的两类模糊Riemann-Stiehjes积分序列的收敛定理.