耐有机溶剂脂肪酶产生菌的筛选和鉴定

从国内各地采取土样分离筛选产耐有机溶剂脂肪酶的新菌株,通过形态和生理生化特征以及系统进化树分析,筛选到的新菌株命名为Pseudomonas aeruginosa CS-2.来自Pseudomonas aeruginosa CS-2的粗脂肪酶液在乙腈中酶活提高,在苯、氯仿、正己烷、石油醚和异辛烷中表现出较高的稳定性.     

Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model

Molecular studies using trace DNA, such as from museum specimens, ancient or forensic samples and samples obtained noninvasively, often have a common problem of low quality of DNA templates. Amplification errors, such as allelic dropout and false allele, may arise during polymerase chain reaction (PCR) using such samples. A mathematical model which treats homozygotes and heterozygotes discriminately has been developed to measure sample quality and compute the confidence level of using multipletube approaches. We use plucked hair samples collected from 26 individual Sichuan snub-nosed monkeys (Rhinopithecus roxel- lana) to test the model. In this case, a confidence level of 99% can be achieved by three positive PCRs. If the sample quality is very poor and requires many PCR replicates, an alternative multiplestep genotyping method is recommended. This model enables researchers to optimize experimental protocols through pilot studies and obtain reliable genetic information using noninvasive sampling method.     

运用ARIMA模型对我国三大产业增加值变动规律进行分析

通过运用ARIMA模型对我国改革开放以来三大产业的增加值变动规律进行分析,旨在探究我国产业结构发展变化的状况是否与世界各国的一般规律相符;此外,通过建立ARIMA模型,探知三大产业增加值的变动是否与其自身有关。     

Temperature-scan cryocrystallography reveals reaction intermediates in bacteriophytochrome

Light is a fundamental signal that regulates important physiological processes such as development and circadian rhythm in living organisms. Phytochromes form a major family of photoreceptors responsible for red light perception in plants, fungi and bacteria. They undergo reversible photoconversion between red-absorbing (Pr) and far-red-absorbing (Pfr) states, thereby ultimately converting a light signal into a distinct biological signal that mediates subsequent cellular responses. Several structures of microbial phytochromes have been determined in their dark-adapted Pr or Pfr states. However, the structural nature of initial photochemical events has not been characterized by crystallography. Here we report the crystal structures of three intermediates in the photoreaction of Pseudomonas aeruginosa bacteriophytochrome (PaBphP). We used cryotrapping crystallography to capture intermediates, and followed structural changes by scanning the temperature at which the photoreaction proceeded. Light-induced conformational changes in PaBphP originate in ring D of the biliverdin (BV) chromophore, and E-to-Z isomerization about the C(15) = C(16) double bond between rings C and D is the initial photochemical event. As the chromophore relaxes, the twist of the C(15) methine bridge about its two dihedral angles is reversed. Structural changes extend further to rings B and A, and to the surrounding protein regions. These data indicate that absorption of a photon by the Pfr state of PaBphP converts a light signal into a structural signal via twisting and untwisting of the methine bridges in the linear tetrapyrrole within the confined protein cavity.     

江苏如东近海绿潮藻分子检测与类群演替分析

2007 年以来, 中国青岛、连云港、如东等黄海沿海连续4 年爆发绿潮现象, 尤其2008 年青岛爆发了世界最大规模绿潮, 造成了严重的生态危害. 2009 年, 我们对江苏省如东海域绿潮藻进行了调查和监测, 选取紫菜养殖架和防波堤坝上的11 个固着样品以及海区15 个漂浮样品, 对其ITS及5.8S rDNA 和叶绿体rbcL 基因序列进行了分子系统发育和类群演替分析. 结果显示, 如东沿岸堤坝和紫菜养殖筏架具有大量固着生长的浒苔类绿潮藻, 其海区漂浮绿潮藻团出现时间也最早,并逐渐北移; ITS 序列分析将如东样品聚为5 个类群, 即Ulva compressa 类群(6 个样品)、Ulva linza-procera-prolifera (LPP)复合体类群(12 个样品)、Ulva flexuosa 类群(3 个样品)、Blidingia sp.类群(3 个样品)以及Urospora spp.类群(2 个样品), 而rbcL 序列较为保守, 26 个样品只聚为4 个类群.DNA 序列分析表明, 如东海区漂浮与固着绿潮藻类群构成相同, 亲缘关系较近, 漂浮绿潮藻优势类群先后出现次序为: U. compressa, U. flexuosa 及LPP, 最终漂浮种与2008 年黄海绿潮优势种的ITS 序列完全相同. 本研究为今后黄海绿潮溯源及其预测防控奠定了重要基础.     

垂直旋转圆盘边缘液体滑移率及液柱形态

旋转圆盘表面液体与壁面之间存在切向相对滑移,液体的转速并不等于圆盘转速.利用高速摄影拍摄圆盘边缘液体形态,用软件Image J测量了液体的滑移率及液体头液滴与液柱的直径比.分析发现,垂直旋转圆盘不同区域的液体滑移率不同,随转速增加,各区域滑移率趋向相同,为10％～13％.圆盘表面的波动、边缘液体的形态会影响液体滑移率的...     

An Agent-based Adaptive Mechanism for Efficient Job Scheduling in Open and Large-scale Environments

Agent-based scheduling refers to applying intelligent agents to autonomously allocate resources to jobs.Decentralized agent-based scheduling approaches have ach...     

白腐菌对焦化废水中吲哚的降解及其机理

选用白腐菌BP降解吲哚,研究了白腐菌在不同培养基中对两种浓度吲哚的降解过程和机理,以及吲哚的降解与白腐菌漆酶酶活、生物量、培养基pH值的关系.结果显示不同培养基中白腐菌可去除99%以上的吲哚;吲哚去除率与白腐菌漆酶活率具有较好的相关性,白腐菌漆酶活率达到最高时,可去除97%以上的吲哚;高浓度吲哚会抑制白腐菌的生长,同时也激发了白腐菌漆酶的产生,秸秆滤出液能促进白腐菌的生长和漆酶活力的增长;白腐菌BP最适pH值为6~7,pH值在5~8之间对吲哚都具有较强的降解能力;在HPLC谱图3~6 min之间出现的新物质可能有氧化吲哚和靛红.     

基于Unity3D的水下机器人半实物仿真系统

基于Unity3D研制了一种由地面控制终端、机器人本体和三维仿真系统3部分组成的水下机器人半实物仿真系统,为水下机器人开发过程中的性能测试、控制算法分析和人员操作培训提供了仿真和测试平台。该系统采用同一地面控制终端作为三维仿真系统和机器人控制系统的统一指令输入源,以实现虚拟对象和机器人本体的同步工作,可对系统进行实时监测、调参。采用多目视觉技术对机器人位置采集,对传感器数据进行卡尔曼滤波处理。机器人运动仿真试验结果表明,机器人实际运动路径和仿真规划路径基本相同,该系统动态响应性和控制系统同步性较好。