RNAi-mediated knockingdown of rlpk2 gene retarded soybean leaf senescence

Leaf senescence that occurs in the last stage of leaf development is a genetically programmed process. It is very significant to elucidate the molecular mechanisms that control the initiation and progression of leaf senescence and the way the senescence signal is transduced. In a previous study on artificially induced soybean leaf senescence, we cloned a novel gene designated rlpk2 (Genbank Accession No. AY687391) that encodes a leucine-rich repeat (LRR) receptor like protein kinase. The expression level of rlpk2 gene was shown to be strongly up-regulated during both the natural leaf senescence process in this report and the artificially induced primary-leaf-senescence process in our previous work. The RNA interference (RNAi)-mediated knocking-down of rlpk2 dramatically retarded both the natural and nutrient deficiency-induced leaf senescence in transgenic soybean. The transgenic leaves showed more cell-aggregated surface structure and higher content of chlorophyll.     

Functional identification of gene cluster for the aniline metabolic pathway mediated by transposable element

A convenient and widely applicable method has been developed to clone aniline metabolic gene cluster in this study. Three positive recombinant plasmids pDA1, pDB2 and pDB11 were cloned from genomic library of aniline degradation strain AD9. The result of aniline dioxygenase （AD） activity and catechol 2,3-oxygenase （C230） activity assay showed that pDA1 and pDB11 contain aniline dioxygenase genes and catechol 2,3-dioxygenase genes, respectively. The sequence analysis of the total 24.7-kb region revealed that this region contains 25 ORFs, of which 17 genes involve metabolism of aniline. In the gene cluster, the first five genes （tadQTA1A2B） and the subsequent gene （tadR1） were predicted to encode a multi-component aniline dioxygenase and a LysR-type regulator, respectively, while the others （tadD1C1D2C2EFGIJKL） were expected to encode metacleavage pathway enzymes for catechol degradation. The gene cluster was surrounded by two IS1071 sequences.     

基于校园网的一卡通系统设计和应用

一卡通系统的设计和应用提高了校园信息化管理水平。从一卡通系统的设计目标、设计原则和设计功能出发,将数字化和智能化的理念运用到一卡通系统的建设中,并就应用中所涉及的情况作了分析。     

发挥教育在西藏和谐社会建设中的重要作用

建设和谐社会是西藏地区与全国其他地区在社会主义建设新时期面临的重大课题,教育作为重要的社会系统,可以为西藏和谐社会建设中做出基础性的贡献。具体说来可以:通过加快发展职业技术教育,培养区域适用人才,促进和谐社会的物质基础建设;通过开展多元文化教育,培养多元文化意识,促进和谐社会的文化基础建设;通过加强法制教育,维护社会稳定,促进和谐社会的法制基础建设;通过加强环境教育,建立合理的人与自然关系,促进和谐社会的环境基础建设。     

水稻广陆矮4号与杂交水稻杂优1号染色体核型分析

对水稻广陆矮4号和杂交水稻杂优1号2个不同品种水稻前中期和正中期的染色体进行了核型分析比较，结果表明两者染色体的臂长、臂比和形态既有相似之处，又存在一定的差异，广陆矮4号水稻的核型公式：2n=2x=24=16m＋8sm，杂交水稻杂优1号的核型公式：2n=2z=24=18m＋6sm．同时结合G-显带技术，对2种水稻进行了比较分析，发现G-显带区别较大。说明水稻不同品种之间存在一定变异．     

链球菌制剂对NOD鼠胰岛瘤细胞株NIT生长影响的体外研究

为了研究A群溶血性链球菌制剂对NOD鼠胰岛细胞瘤细胞株NIT体外生长的影响,利用不同浓度的制剂处理体外培养的NOD鼠胰岛细胞瘤细胞株NIT和正常小鼠胰岛β细胞,以不加药物的培养孔作为对照来对细胞进行形态学观察.以MTT法检测细胞活性,经Agarose gel电泳分析DNA ladder条带检测凋亡,结果表明：A群溶血性链球菌制剂能显著抑制NIT细胞增殖,而对正常胰岛β细胞没有影响,且该制剂有促NIT细胞凋亡的作用.     

分子导线：半导体性-金属性组合的双壁纳米碳管

由于双壁纳米碳管独特的双层结构,可望在纳米器件领域得到广泛应用。如根据构成两层碳管的导电性不同,可用作分子导线和记忆功能的分子存贮器等纳米电子器件,并可用作分子轴承、分子马达、超高频分子振荡器等纳米机械器件。同时,双壁纳米碳管的双层结构也为研究层间作用力对声子和电子结构的影响提供了理想的平台。但是迄今为止,所制备的双壁纳米碳管由于纯度低、无序排列和直径分布较宽的特点,严重阻碍了对其深入的理论研究和实际应用。沈阳材料科学国家(联合)实验室先进炭材料研究部任文才等于2002年率先提出了流动催化剂化学气相沉积法用…     

喀纳斯河大桥方案设计

喀纳斯河大桥是一座旅游风景区内集交通功能和景观功能于一体的桥梁。文中通过对其方案的设计理念、指导思想的综述,提出两个设计方案并进行比较。     

组合变形梁危险截面

从教学角度通过计算对危险截面位置的确定,并对其进行了证明。     

Continuum-based slip model and its validity for micro-channel flows

New fabrication techniques have been successfully applied to the fabrication of micro devices, such as Mi- cro Electro Mechanical Systems (MEMS), MicroChinese Science Bulletin Vol. 51 No. 9 May 2006 Energy Systems (MES) and so on[1―3], providing op- port…