医用细菌纤维素的制备及其细胞相容性

以椰汁作为基本的碳源和生长因子来源,通过添加成分明确的乙醇及酵母膏作为补充碳源和氮源,并补加相应的微量元素,获得了成分简单、明确的医用细菌纤维素培养基;该培养基各成分含量相对稳定,提高了细菌纤维素的发酵速率和产量,所获得的产物厚度可满足不同的医用需求.将碱液分别与十二烷基硫酸钠(SDS)、超声波及乙醇联用对发酵产物进行纯化处理.结果表明:碱液与SDS联用时纯化效果最好;纯化所得样品的杂质N含量低于检测限,透光率高,在可见光范围内与活体角膜透光率相近,L929细胞相容性良好.     

地下钢筋混凝土岔管围岩稳定及结构数值分析

以国内某抽水蓄能电站的钢筋混凝土岔管工程为例,从围岩开挖支护、围岩与衬砌相互作用和钢筋混凝土结构等方提出了围岩、混凝土等材料的数值模拟方法,通过三维有限元线性和非线性计算,分析研究了地下钢筋混凝土岔管在施工、运行等工况下,围岩、衬砌结构的应力与变形,以及混凝土岔管结构配筋和配筋后应力、裂缝开展等问题.结果表明：在开挖阶段由于地应力释放在主、岔洞交叉处会产生拉应力,塑性区开展较深;正常运行工况在内水压力作用下岔管结构大部分区域存在拉应力,裂缝较大;检修工况在外水压力作用下仅在主、岔管交叉处存在拉应力区,裂缝较小;结构配筋时,轴向配筋应该由外水作用的工况来控制,环向配筋由内水作用为主的工况来控制.     

1000 mm2 cross-section wire a led four tension exhibition put to traction practice

文章介绍了宁东一山东±660 kV直流输电示范工程(晋4标段)1 000 mm2大截面导线一牵四张力展放施工中,使用4个转向滑车转向布置牵引场的实践过程及施工中的注意事项.     

小学应用题教学的一般技巧

本文总结了小学应用题教学中的一般规律,只要我们能领会它的规律,遵循小学生思维发展的规律和认知特点,在教学中结合本班学生基础能力的实际情况,灵活变通地开展教学活动,定能培养起学生的学习兴趣,有效地提高学生解答应用题的能力.     

PTC124 targets genetic disorders caused by nonsense mutations

Nonsense mutations promote premature translational termination and cause anywhere from 5-70% of the individual cases of most inherited diseases. Studies on nonsense-mediated cystic fibrosis have indicated that boosting specific protein synthesis from <1% to as little as 5% of normal levels may greatly reduce the severity or eliminate the principal manifestations of disease. To address the need for a drug capable of suppressing premature termination, we identified PTC124-a new chemical entity that selectively induces ribosomal readthrough of premature but not normal termination codons. PTC124 activity, optimized using nonsense-containing reporters, promoted dystrophin production in primary muscle cells from humans and mdx mice expressing dystrophin nonsense alleles, and rescued striated muscle function in mdx mice within 2-8 weeks of drug exposure. PTC124 was well tolerated in animals at plasma exposures substantially in excess of those required for nonsense suppression. The selectivity of PTC124 for premature termination codons, its well characterized activity profile, oral bioavailability and pharmacological properties indicate that this drug may have broad clinical potential for the treatment of a large group of genetic disorders with limited or no therapeutic options.     

区域城乡协调发展的综合评价——以宁波为例

城乡之间的协调表现在很多方面.要全面、客观地评价一个地区的城乡协调发展情况,必须建立科学合理的评价指标体系,选择恰当的评价方法.本文结合宁波市的实际情况,讨论了城乡协调发展的内涵和目标,提出了城乡协调发展评价指标体系和方法,揭示了宁波市近年城乡协调发展方面存在的问题和未来可能的变化态势.