基于超像素分割的视频目标检索

针对视频监控中的颜色特征检索,提出一种基于超像素分割的视频目标检索算法:该算法首先对视频帧序列按颜色及空间位置进行超像素分割,分割成若干个超像素区域;然后对每个超像素区域颜色值进行均值化处理;最后基于超像素区域进行颜色目标检索.为了验证算法的有效性,以监控拍摄的户外视频数据进行仿真实验,实验结果表明该算法能准确检索出所...     

细胞太空无源搭载装置中的微载体技术初步研究

探讨基于微载体技术的改良细胞单克隆化方法,及直接研究微载体上细胞形态、细胞增殖、核酸提取的方法。利用快速染色法直接观察微载体上的细胞形态,并用MTT法直接分析细胞增殖,用Trizol试剂和吸附柱直接从微载体提取细胞核酸。对微载体上的细胞可直接进行染色,观察正常细胞和凋亡细胞的形态。MTT分析显示细胞数和OD值呈直线关系。从微载体上直接提取的RNA完整性好,可用于RT-PCR分析,提取的DNA含量较高。改良细胞单克隆化方法操作简便,细胞活性好;不消化细胞,可以直接研究微载体上的细胞形态、细胞增殖,提取核酸。     

加Ar权和Aλr权的H(o)lder型不等式

积分不等式用来估计积分值非常有力,利用权得到了广义H(o)lder不等式的推广:加Ar权和Aλr权的积分不等式,该不等式可被用来研究积分性质和用来估计积分值.     

Erratum to: <Emphasis Type="Italic">In vitro</Emphasis> induction of mouse meningeal-derived iPS cells into neural-like cells

The authors report that incorrect figures were included as Figures 4 and 5 in the original publication of this paper. This error occurred during the figure editing process of the publishing company. This information should have been presented as follows:     

不同恶化程度胃癌细胞系的细胞间隙连接通讯功能及Cx43表达的差异

以人正常胃黏膜上皮细胞系GES-1和不同恶化程度的人胃癌细胞系(低恶化AGS细胞系、中恶化SGC-7901细胞系、高恶化BGC-823细胞系)为研究对象,采用激光共聚焦显微镜的荧光漂白恢复技术及划痕标记荧光染料示踪技术研究不同恶化程度的胃癌细胞系细胞间隙连接通讯(Gap junctional intercellular communication,GJIC)功能的差异;采用荧光定量PCR技术和间接免疫荧光技术检测间隙连接蛋白(Connexin43,Cx43)基因在mRNA和蛋白水平的表达差异,探求其与胃癌恶性程度的相关性.结果表明,在正常胃细胞系和低、中及高恶化胃腺癌细胞系中,细胞间的GJIC功能随着恶性程度的升高而减弱(AGS、SGC-7901)或消失(BGC-823),且Cx43蛋白及mRNA的表达量随着恶性程度的升高而下调(AGS、SGC-7901)或缺失(BGC-823).这提示胃癌恶性程度与胃癌细胞的GJIC功能显著相关(P<0.05),GJIC在胃癌发生中起重要作用.     

Molecular regulation of sexual preference revealed by genetic studies of 5-HT in the brains of male mice

Although the question of to whom a male directs his mating attempts is a critical one in social interactions, little is known about the molecular and cellular mechanisms controlling mammalian sexual preference. Here we report that the neurotransmitter 5-hydroxytryptamine (5-HT) is required for male sexual preference. Wild-type male mice preferred females over males, but males lacking central serotonergic neurons lost sexual preference although they were not generally defective in olfaction or in pheromone sensing. A role for 5-HT was demonstrated by the phenotype of mice lacking tryptophan hydroxylase 2 (Tph2), which is required for the first step of 5-HT synthesis in the brain. Thirty-five minutes after the injection of the intermediate 5-hydroxytryptophan (5-HTP), which circumvented Tph2 to restore 5-HT to the wild-type level, adult Tph2 knockout mice also preferred females over males. These results indicate that 5-HT and serotonergic neurons in the adult brain regulate mammalian sexual preference.     

两阶段非线性Wiener过程退化建模与可靠性分析

基于性能退化数据对产品进行可靠性分析一直是可靠性工程领域研究的热点与难点。针对多阶段退化过程中存在的非线性规律,同时考虑变点位置位于测试时刻之间的一般情形且各试样变点存在个体差异性的实际情况,提出了基于Wiener过程的两阶段非线性退化模型,给出了模型参数极大似然估计方法,建立了可靠性分析和寿命预测方法。最后通过高压脉冲电容器实例的对比分析,验证了所提方法的合理性与有效性。结果表明,与多阶段线性Wiener过程方法相比,所提方法能够更加准确地描述退化过程的非线性时变特征,进而得到更为合理的寿命评估结果。     

Mechanical behavior of a near α titanium alloy under dynamic compression: Characterization and modeling

Dynamic compression tests under strain rates from 870 s^?1 to 2100 s^?1 were conducted for a near α Ti–8Al–1Mo–1V titanium alloy with equiaxed microstructure. Compression behavior, adiabatic shearing and band microstructure were investigated via characterization and calculation. The results demonstrate that all dynamic constitutive curves exhibited obvious stress fluctuation phenomenon with double increase-decrease changing stages at the primary stage of compression. The dislocation multiplication theory can be used to explain this phenomenon. After the stress fluctuation period, work hardening coexisted with the thermal softening, resulting in the slow hardening tendency in constitutive curves. J-C model was utilized to quantify the dynamic constitutive curves. The deviations between the predicted and experimental curves under high strain rates may be attributed to the over-consideration of thermal softening effect in J-C model. Adiabatic shearing band (ASB) began to form under the strain rate of 2100 s^?1. A total shearing strain of 8.1 within ASB achieved in 8.9 μs, corresponding to a local strain rate of about 9.1 × 10⁵ s^?1 and is over 430 times of the macro strain rate. Post annealing was conducted on ASB before EBSD characterization. Due to the static recrystallization during annealing, the α phase within ASB generally presented as ultra-fine grains less than 1 μm in diameter.     

VOx·nH2O纳米管的制备及表征

通过表面活性剂乳化剂OP-10/十八胺作为软模板在水热条件下合成均一、超长VOx·nH2 O纳米管.纳米管长5-15μm,外径为100-120mm,内径10-20nm.所得产物用X-射线衍射(XRD)、透射电子显微镜(TEM)高分辨电子显微镜(HRTEM)进行表征,并对其反应机理进行了研究.     

Plasmonic biosensors and nanoprobes based on gold nanoshells

Gold nanoshells (GNSs), consisting of a dielectric core coated with gold, have gained extensive attention as they show readily tunable optical properties and good biocompatibility. As highly sensitive and label-free optical biosensors with wide applications, GNSs have been investigated in many fields including drug delivery, immunoassay, cancer treatment, biological sensing and imaging. Taking advantage of the adjustability of the local surface plasmon resonance (LSPR) and the sensitivity of the surfaceenhanced Raman scattering (SERS) signal of GNSs, we have developed diverse applications including plasmonic biosensors and nanoprobes based on GNSs. In this review we introduce plasmonic and electromagnetic properties and fabrication methods of GNSs. We describe research progress in recent years, and highlight several applications of GNSs developed by our group. Finally we provide a brief assessment of future development of GNSs as plasmonic materials that can be integrated with complementary analytical techniques.