(6,5)单壁碳纳米管对盐酸阿霉素的载药研究

以分离后的单壁碳纳米管为载药工具,制备了一种(6,5)单壁碳纳米管-盐酸阿霉素-人血清白蛋白-叶酸((6,5)SWCNT-Dox-HSA-FA)四元靶向载药系统.首先通过反应制备得到三元复合物Dox-HSA-FA,并采用双水相萃取法分离得到(6,5)SWCNT;然后采用超声作用将(6,5)SWCNT分散至Dox-HSA...     

Genome-wide copy number variation study associates metabotropic glutamate receptor gene networks with attention deficit hyperactivity disorder

Attention deficit hyperactivity disorder (ADHD) is a common, heritable neuropsychiatric disorder of unknown etiology. We performed a whole-genome copy number variation (CNV) study on 1,013 cases with ADHD and 4,105 healthy children of European ancestry using 550,000 SNPs. We evaluated statistically significant findings in multiple independent cohorts, with a total of 2,493 cases with ADHD and 9,222 controls of European ancestry, using matched platforms. CNVs affecting metabotropic glutamate receptor genes were enriched across all cohorts (P = 2.1 × 10(-9)). We saw GRM5 (encoding glutamate receptor, metabotropic 5) deletions in ten cases and one control (P = 1.36 × 10(-6)). We saw GRM7 deletions in six cases, and we saw GRM8 deletions in eight cases and no controls. GRM1 was duplicated in eight cases. We experimentally validated the observed variants using quantitative RT-PCR. A gene network analysis showed that genes interacting with the genes in the GRM family are enriched for CNVs in ～10% of the cases (P = 4.38 × 10(-10)) after correction for occurrence in the controls. We identified rare recurrent CNVs affecting glutamatergic neurotransmission genes that were overrepresented in multiple ADHD cohorts.     

CCDC103 mutations cause primary ciliary dyskinesia by disrupting assembly of ciliary dynein arms

Cilia are essential for fertilization, respiratory clearance, cerebrospinal fluid circulation and establishing laterality. Cilia motility defects cause primary ciliary dyskinesia (PCD, MIM244400), a disorder affecting 1:15,000-30,000 births. Cilia motility requires the assembly of multisubunit dynein arms that drive ciliary bending. Despite progress in understanding the genetic basis of PCD, mutations remain to be identified for several PCD-linked loci. Here we show that the zebrafish cilia paralysis mutant schmalhans (smh(tn222)) encodes the coiled-coil domain containing 103 protein (Ccdc103), a foxj1a-regulated gene product. Screening 146 unrelated PCD families identified individuals in six families with reduced outer dynein arms who carried mutations in CCDC103. Dynein arm assembly in smh mutant zebrafish was rescued by wild-type but not mutant human CCDC103. Chlamydomonas Ccdc103/Pr46b functions as a tightly bound, axoneme-associated protein. These results identify Ccdc103 as a dynein arm attachment factor that causes primary ciliary dyskinesia when mutated.     

The coiled-coil domain containing protein CCDC40 is essential for motile cilia function and left-right axis formation

Primary ciliary dyskinesia (PCD) is a genetically heterogeneous autosomal recessive disorder characterized by recurrent infections of the respiratory tract associated with the abnormal function of motile cilia. Approximately half of individuals with PCD also have alterations in the left-right organization of their internal organ positioning, including situs inversus and situs ambiguous (Kartagener's syndrome). Here, we identify an uncharacterized coiled-coil domain containing a protein, CCDC40, essential for correct left-right patterning in mouse, zebrafish and human. In mouse and zebrafish, Ccdc40 is expressed in tissues that contain motile cilia, and mutations in Ccdc40 result in cilia with reduced ranges of motility. We further show that CCDC40 mutations in humans result in a variant of PCD characterized by misplacement of the central pair of microtubules and defective assembly of inner dynein arms and dynein regulatory complexes. CCDC40 localizes to motile cilia and the apical cytoplasm and is required for axonemal recruitment of CCDC39, disruption of which underlies a similar variant of PCD.     

基于主成分回归分析的瓦斯含量预测

为了增加多元回归模型预测的精度,将主成分分析与多元回归分析相结合提出了PCA—MRA模型,并将该模型用于实际瓦斯含量预测。结果表明,PCA—MRA模型消除了输入变量之间的相关性,减少了输入变量值个数,提高了预测精度,便于实际推广和应用,为瓦斯含量预测提供一种新的途径。     

加快人才培养促进科技创新为"奋战五年,再造安吉"提供智力支撑

科技是第一生产力,人才是第一资源,要在激烈的竞争中立于不败之地,实现县域经济又好又快发展,没有一大批高素质人才,特别是创新人才的支撑,显然是不可想象的。加快人才的培养与开发,既是站在新的历史起点,推进创新型社会建设的内在要     

用于控制系统研究的300MW机组锅炉动态模型

依据系统机理推导了300MW机组锅炉的动态模型.模型的输入为给水阀开度、汽机调门开度、燃料量、给水温度;输出为汽包压力、汽包水位、蒸汽流量.模型中各系数与运行工况有关,可根据当前的汽包压力、锅炉物理结构数据及水蒸汽性质得到.该模型反映了实际系统的时变性及非线性,可用于基于模型的锅炉控制算法的研究,也可用于火电站实时仿真机上.     

高层建筑防雷设计与施工技术

高层建筑的防雷工程十分重要。文章探讨了高层建筑防雷设计问题，并分析了高层建筑防雷的施工与技术。     

高校教学设备计划管理工作的探讨

从高校仪器设备管理的第一个环节设备计划管理入手,结合西南石油大学实验室建设与设备管理的实际情况,利用科学的管理方法,努力提高教学设备计划申报的有效性、准确性、可靠性,进而提高设备的投资效益.