利用脊线检测实现视网膜图像血管中心线的精确提取

提出一种精确有效的基于脊线检测的视网膜图像血管中心线提取算法,该算法首先对原始图像进行脊线检测得到候选视网膜图像血管中心线,然后对原始图像进行照度均衡和多尺度形态学增强处理,最后对各个尺度增强图像在视场ROI区域内进行Otsu双阈值分割或单阈值分割,并将各个尺度的分割结果求和再与脊线检测结果算术相与得到最终的血管中心线。通过对25张荧光造影视网膜图像以及部分彩色视网膜图像进行测试和分析,该算法不但能够检测出低对比度血管和微小血管的中心线,而且提取的血管中心线整体连续性好。将文中算法的结果与血管手动分割血管的细化结果进行比对分析,中心线吻合率平均达到83.5%,且算法性能优于Hoover算法以及保守专家手动检测结果。     

并行埋地管道准周期土壤温度场的数值模拟

并行埋地管道中,常温输送的成品油管道会影响热原油管道的温度场,从而与单根输油管道的温度场不同.热油管道周围土壤温度场是管道停输再启动和管道运行的基础,只有准确掌握管道周围土壤温度场的变化规律,才能使管道安全运行,避免凝管事故发生.为了研究并行管道周围土壤温度场在准周期内的变化规律,以西部原油成品油管道四堡进站位置为例,在实测管道周围土壤物性的基础上,采用非结构化有限容积法对并行埋地管道周围土壤温度场进行模拟研究.模拟计算结果与现场实测结果基本吻合,表明提出的计算模型正确,计算结果能够为工程实际提供参考.     

排列中相邻两项的最大公因子

1983年,Erds P,Freud R和Hegyvári N证明了对所有正整数的任一排列a1,a2,a3,…,有liminfi(ai,ai+1)/i≤61/90.本文将结果改进为liminfi(ai,ai+1)/i≤13/20.     

Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model

Molecular studies using trace DNA, such as from museum specimens, ancient or forensic samples and samples obtained noninvasively, often have a common problem of low quality of DNA templates. Amplification errors, such as allelic dropout and false allele, may arise during polymerase chain reaction (PCR) using such samples. A mathematical model which treats homozygotes and heterozygotes discriminately has been developed to measure sample quality and compute the confidence level of using multipletube approaches. We use plucked hair samples collected from 26 individual Sichuan snub-nosed monkeys (Rhinopithecus roxel- lana) to test the model. In this case, a confidence level of 99% can be achieved by three positive PCRs. If the sample quality is very poor and requires many PCR replicates, an alternative multiplestep genotyping method is recommended. This model enables researchers to optimize experimental protocols through pilot studies and obtain reliable genetic information using noninvasive sampling method.     

三角褐指藻（Phaeodactylum tricornutum）油脂富集培养的研究

探讨了营养盐铁、硅及植物激素脱落酸(ABA)单因子对三角褐指藻（Phaeodactylum tricornutum）的生长、油脂含量（%干重）及油脂产量的影响.采用光密度值OD450评价微藻的生长状况,以溶剂浸提法提取油脂.结果表明：⑴铁处理组中,铁浓度在0~46.57 μmol/L范围内时,藻的生长速率随铁浓度提高而增大;铁浓度为11.64 μmol/L时,油脂含量达30%,高于其它铁浓度培养;当铁浓度为11.64 μmol/L时,油脂产量最高——是无铁培养的2.8倍; ⑵ 硅处理组中,低含量硅盐（     

高频CO2激光脉冲写入的掺硼长周期光纤光栅温度应变特性

高频CO2激光脉冲写入的长周期光纤光栅(LPFG)对温度比较敏感、应变不敏感,通常应用在温度传感器做温度测量.普通长周期光纤光栅温度灵敏度只有0.052 nm·℃-1,而用掺硼光纤制作的长周期光纤光栅温度灵敏度达到0.171 nm·℃-1,比普通长周期光纤光栅更加灵敏.因而应用在温度传感提高了传感器的灵敏度以及测温精度...     

可跨膜SOD对肝癌及正常肝细胞增殖的作用

可跨膜融合蛋白PTD-SOD与肝癌细胞和正常肝细胞共培养,使细胞内SOD活力分别增加了52%和23%,提高胞内总抗氧化能力(T-AOC)水平达100%,同时降低ROS水平.实验结果显示,肝癌细胞增殖受到显著抑制,最高抑制率达91.91%,对正常肝细胞抑制率较小,为45.50%.     

黑龙江七星河国家级湿地自然保护区生态旅游开发的SWOT分析

采用SWOT分析法较为详细地分析了七星河国家级湿地自然保护区发展生态旅游业的优势、弱势、机遇和威胁。得出黑龙江七星国家级河湿地自然保护区不仅具备景观资源独特、动植物资源丰富、旅游环境优越等开展湿地生态旅游的先决条件,而且拥有国内外生态旅游发展的有利形势和地方政策大力支持的大好机遇;但同时存在湿地保护和专业人员匮乏、旅游...     

基于并行遗传算法的舰艇编队防空火力分配方案

设计了一种并行遗传算法,并进行了仿真实验．结果表明,该算法可以有效求得舰艇编队防空火力分配问题的最优解或近似最优解．     

Structural insight into brassinosteroid perception by BRI1

Brassinosteroids are essential phytohormones that have crucial roles in plant growth and development. Perception of brassinosteroids requires an active complex of BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and BRI1-ASSOCIATED KINASE 1 (BAK1). Recognized by the extracellular leucine-rich repeat (LRR) domain of BRI1, brassinosteroids induce a phosphorylation-mediated cascade to regulate gene expression. Here we present the crystal structures of BRI1(LRR) in free and brassinolide-bound forms. BRI1(LRR) exists as a monomer in crystals and solution independent of brassinolide. It comprises a helical solenoid structure that accommodates a separate insertion domain at its concave surface. Sandwiched between them, brassinolide binds to a hydrophobicity-dominating surface groove on BRI1(LRR). Brassinolide recognition by BRI1(LRR) is through an induced-fit mechanism involving stabilization of two interdomain loops that creates a pronounced non-polar surface groove for the hormone binding. Together, our results define the molecular mechanisms by which BRI1 recognizes brassinosteroids and provide insight into brassinosteroid-induced BRI1 activation.