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Small area analysis of sociodemographic change over time is often impeded by the changing configuration of census zones for each decennial UK census. Approaches for matching zone configurations exist, but these typically require population estimation techniques, which inevitably involve some error. Because of the postcode-based geographical zoning system used in Scottish censuses, it is possible to create a local-area geography which is consistent for recent censuses. Here we present a methodology used to create three sets of consistent areas through time (CATTs) that can be used for analysing comparable small area data output from the 1981, 1991 and 2001 Censuses. 相似文献
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BOTTLENECKS IN PRODUCTION NETWORKS: AN OVERVIEW 总被引:1,自引:0,他引:1
Yongcai WANG Qianchuan ZHAO Dazhong ZHENG Center for Intelligent Networked Systems Department of Automation Tsinghua University Beijing P.R.China 《系统科学与系统工程学报(英文版)》2005,14(3):347-363
1. Introduction Performances of a production system, such as the throughput, the circle time and the average delay, etc., are affected by the capacities of machines and resources available in the system. Some of them may affect the system performances more than others. Usually, the limitation of a system can be traced to the limitation of one or two machines or one or two kinds of resources, commonly called bottlenecks. From system point of view, bottlenecks are the congestion points of the sy… 相似文献
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Human peripheral blood monuclear cells transfected with messenger RNA stimulate antigen-specific cytotoxic T-lymphocytes in vitro 总被引:1,自引:0,他引:1
Teufel R Carralot JP Scheel B Probst J Walter S Jung G Hoerr I Rammensee HG Pascolo S 《Cellular and molecular life sciences : CMLS》2005,62(15):1755-1762
The efficiency of test vaccines needs to be evaluated by quantification of the triggered cellular immune response. Usually, for these assays, autologous target cells expressing the vaccine antigen are required. In the context of messenger RNA (mRNA)-based vaccinations, the target cells used for the read-out are mRNA-transfected monocyte-derived dendritic cells (Mo-DCs). Their production typically requires samples of 100 ml blood from the patients, and limits the number of assays that can be performed. We show here that fresh peripheral blood mononuclear cells (PBMCs) can be transfected with mRNA by electroporation. Such cells are as efficient as mRNA-transfected Mo-DCs for their ability to activate memory T cells in vitro. Thus, mRNA-transfected PBMCs are a convenient replacement of mRNA-transfected Mo-DCs for the in vitro monitoring of natural or vaccine-induced immune responses.Received 17 February 2005; received after revision 1 May 2005; accepted 7 Juni 2005 相似文献
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