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981.
The Tetrahymena ribozyme acts like an RNA restriction endonuclease 总被引:41,自引:0,他引:41
A shortened form of the Tetrahymena self-splicing ribosomal RNA intervening sequence acts as an endoribonuclease, catalysing the cleavage of large RNA molecules by a mechanism involving guanosine transfer. The sequence specificity approaches that of the DNA restriction endonucleases. Site-specific mutagenesis of the enzyme active site alters the substrate sequence specificity in a predictable manner, so that endoribonucleases can be synthesized to cut at a variety of tetranucleotide sequences. 相似文献
982.
Loss of a Harvey ras allele in sporadic Wilms' tumour 总被引:5,自引:0,他引:5
A E Reeve P J Housiaux R J Gardner W E Chewings R M Grindley L J Millow 《Nature》1984,309(5964):174-176
Genomic changes within chromosome band 11p13 appear to have a role in the initiation of Wilms' tumour. The human Harvey ras oncogene, c-Ha-ras 1, has been located by Jhanwar et al. immediately adjacent to this region at band 11p14 .1, although several groups have assigned the gene more distally at band 11p15 . We have examined tumour DNA from two cases of sporadic Wilms' tumour, and report here that in both cases one of the two constitutional c-Ha-ras 1 alleles was absent. One tumour had a reciprocal translocation between the short arm of chromosome 11 (at band 11p13), and the long arm of chromosome 12, with no visible loss of chromosomal material. The loss of a c-Ha-ras 1 allele in association with this translocation indicates that a submicroscopic deletion had occurred. The resulting hemizygosity may have had a role in tumour initiation. Our results indicate that the c-Ha-ras 1 gene and the 'Wilms' tumour locus' may be in close proximity. It would, therefore, be premature to exclude the possibility that these two sites are functionally related. 相似文献
983.
研制了一台动态范围为90dB以上的环境噪声自动监测及统计分析仪器。可以适用于声级起伏变化很大的环境噪声和交通噪声的全量程监测,并可以把测量结果和统计分析结果自动显示和打印出来,解决了随机性较强的环境噪声的自动监测问题。仪器內部有两只对数放大器,由于器件特性的限制,每只对数放大器的动态范围约60dB左右,但通过微处理器对双通道对数放大器的交替采样,并经过数据处理,实现了二对数放大器的测量动态范围的自动连接,这样扩大了量程(大于90dB)实现了仪器的全量程测试。 相似文献
984.
G. Planz J. Bieber H. J. Mencke G. v. Bernuth 《Cellular and molecular life sciences : CMLS》1983,39(5):497-498
Summary Plasma catecholamine concentrations in 46 children of various ages were determined by a sensitive radioenzymatic assay. Noradrenaline levels were found to be in the same range as in adults, whereas adrenaline levels in a few of the children were abnormally high. 相似文献
985.
Effector T lymphocyte line cells migrate to the thymus and persist there 总被引:15,自引:0,他引:15
Y Naparstek J Holoshitz S Eisenstein T Reshef S Rappaport J Chemke A Ben-Nun I R Cohen 《Nature》1982,300(5889):262-264
986.
Molecular structure of r(GCG)d(TATACGC): a DNA--RNA hybrid helix joined to double helical DNA 总被引:20,自引:0,他引:20
A H Wang S Fujii J H van Boom G A van der Marel S A van Boeckel A Rich 《Nature》1982,299(5884):601-604
The molecule r(GCG)d(TATACGC) is self-complementary and forms two DNA--RNA hybrid segments surrounding a central region of double helical DNA; its molecular structure has been solved by X-ray analysis. All three parts of the molecule adopt a conformation which is close to that seen in the 11-fold RNA double helix. The conformation of the ribonucleotides is partly determined by water molecules bridging between the ribose O2' hydroxyl group and cytosine O2. The hybrid-DNA duplex junction contains no structural discontinuities. However, the central DNA TATA sequence has some structural irregularities. 相似文献
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