悬索桥上部结构的抗震设计

大跨悬索桥必须进行可靠的抗震设计。由于悬索桥第一自振周期长达几十ｓ,有多达百余个自振周期密集在０．５～５ｓ区段内,现行桥梁抗震规范不适用于大跨悬索桥。配合交通部“公路悬索桥设计规范”编制的研究工作,讨论了反应谱５ｓ以上长周期段、反应谱振型分解法的振型组合方式及应组合的振型数、以及时程积分时的地震记录的持续时间对大跨悬索桥地震反应的影响,发现它们的影响十分显著。从而对悬索桥上部结构抗震设计的某些要点提出建议。     

汽车点火系统电磁干扰的模拟仿真

点火系统工作时初级回路的瞬变电压引起的传导干扰将对蓄电池电压造成冲击,并通过导线干扰车内ECU和其它电子设备的正常工作;同时火花塞间隙击穿时的火花电流噪声对车内形成极强的辐射干扰.根据点火过程中初级电路和次级回路建立的电路模型,计算了点火线圈初级电压、电流在开关闭合到断开过程中的波形变化和次级回路的火花电流,并将高压导线等效为单极天线计算了在车内的辐射电场分布,比较了高压导线的工作频率和终端负载的影响.研究表明,采用阻尼导线和增大火花塞内电阻都可以有效抑制火花电磁噪声.     

The diploid genome sequence of an Asian individual

Here we present the first diploid genome sequence of an Asian individual. The genome was sequenced to 36-fold average coverage using massively parallel sequencing technology. We aligned the short reads onto the NCBI human reference genome to 99.97% coverage, and guided by the reference genome, we used uniquely mapped reads to assemble a high-quality consensus sequence for 92% of the Asian individual's genome. We identified approximately 3 million single-nucleotide polymorphisms (SNPs) inside this region, of which 13.6% were not in the dbSNP database. Genotyping analysis showed that SNP identification had high accuracy and consistency, indicating the high sequence quality of this assembly. We also carried out heterozygote phasing and haplotype prediction against HapMap CHB and JPT haplotypes (Chinese and Japanese, respectively), sequence comparison with the two available individual genomes (J. D. Watson and J. C. Venter), and structural variation identification. These variations were considered for their potential biological impact. Our sequence data and analyses demonstrate the potential usefulness of next-generation sequencing technologies for personal genomics.     

新型投影式顶焦度计的结构原理及使用

顶焦度计又称对光机、查片机,可用来测量球光镜镜度、散光镜镜度、棱镜镜度以及散光轴线和光学中心点等,是眼镜片冷加工中必不可少的检测仪器。目前常用的顶焦度计有两种：一种为读数投影到影屏上,即投影式顶焦度计;另一种为目镜观察图像,即目镜观察式顶焦度计;其中投影式顶焦度计是目前普遍使用的机型。对投影式顶焦度计的结构原理及使用的阐述,为专业人员的临床使用与培养分析提供了技术参数、技术路线与技术规范。     

东北老工业基地会展业发展效应及定位分析

会展业的发展是随着经济与社会的发展而产生的,它依托城市的现实基础,同时为城市及所在区域带来显著的经济效益和社会效益,并带动旅游、服务等相关行业的发展,具有明显的产业带动效应和产业聚集效应。因此,为加快东北老工业基地的建设,应借助东北地区尤其是吉林省的独特优势大力发展会展业,并借助香港会展业成功的经验走国际化和专业化的道路,从而带动地方经济和东北老工业基地区域经济的繁荣。     

我国特有的三个小型猪品系PGC-1基因外显子8的多态性分析

目的 分析我国特有的三个小型猪品系:巴马小型猪、中国农大小型猪、五指山小型猪过氧化物酶体增殖物激活受体γ协同激活子1基因第8外显子的单核苷酸多态性(SNPs)分布特点,为我国小型猪在糖尿病和代谢性疾病研究中提供基础资料.方法 以三个品系小型猪基因组DNA为模板,应用特异性引物进行PCR扩增,扩增产物纯化测序,进行BLAST比对分析.结果 测序结果表明在小型猪PGC-1基因外显子8有5个单核苷酸多态性位点为:Ala354Val(450C→T),Gly363Gly(478C→T),Arg369Arg(494C→A),Leu570Leu(913G→A),Ser551Ser(1039A→G),其中,只有450位C→T的杂合突变导致了编码氨基酸的改变.结论 小型猪品种不同PGC-1基因外显子8的多态性分布有很大差异.     

基于广播模式的录井仪实时数据传输系统的设计与实现

随着通信技术发展,应用Socket通信技术进行综合录井数据的远程传输,可以将现场录井数据信息实时传送到基地,为油田提供集录井现场信息采集、远程信息传输、基地信息接收处理为一体化的信息服务.本文描述卫星实时数据传输系统及应用研究建设项目中的数据采集模块的设计过程.在此过程中使用了VC 及Delphi两种IDE,采用了动态链接库及多线程技术来完成Socket通信,该系统具备采集、传输和接收实时数据的功能.     

长输管道SCADA系统的无线通信方法分析与改进

根据目前参与的长输管道控制项目,结合管道控制的实际情况,对目前国内外无线SCA-DA系统进行比较.同时针对各种管道无线SCADA系统存在的不足提出了一些解决方法,从而进一步完善了管道无线SCADA系统,提高了无线SCADA系统的运行性能.     

Structure of the CED-4-CED-9 complex provides insights into programmed cell death in Caenorhabditis elegans

Interplay among four genes--egl-1, ced-9, ced-4 and ced-3--controls the onset of programmed cell death in the nematode Caenorhabditis elegans. Activation of the cell-killing protease CED-3 requires CED-4. However, CED-4 is constitutively inhibited by CED-9 until its release by EGL-1. Here we report the crystal structure of the CED-4-CED-9 complex at 2.6 A resolution, and a complete reconstitution of the CED-3 activation pathway using homogeneous proteins of CED-4, CED-9 and EGL-1. One molecule of CED-9 binds to an asymmetric dimer of CED-4, but specifically recognizes only one of the two CED-4 molecules. This specific interaction prevents CED-4 from activating CED-3. EGL-1 binding induces pronounced conformational changes in CED-9 that result in the dissociation of the CED-4 dimer from CED-9. The released CED-4 dimer further dimerizes to form a tetramer, which facilitates the autoactivation of CED-3. Together, our studies provide important insights into the regulation of cell death activation in C. elegans.