Functional characterization of the human α-cardiac actin mutations Y166C and M305L involved in hypertrophic cardiomyopathy

Inherited cardiomyopathies are caused by point mutations in sarcomeric gene products, including α-cardiac muscle actin (ACTC1). We examined the biochemical and cell biological properties of the α-cardiac actin mutations Y166C and M305L identified in hypertrophic cardiomyopathy (HCM). Untagged wild-type (WT) cardiac actin, and the Y166C and M305L mutants were expressed by the baculovirus/Sf9-cell system and affinity purified by immobilized gelsolin G4-6. Their correct folding was verified by a number of assays. The mutant actins also displayed a disturbed intrinsic ATPase activity and an altered polymerization behavior in the presence of tropomyosin, gelsolin, and Arp2/3 complex. Both mutants stimulated the cardiac β-myosin ATPase to only 50?% of WT cardiac F-actin. Copolymers of WT and increasing amounts of the mutant actins led to a reduced stimulation of the myosin ATPase. Transfection of established cell lines revealed incorporation of EGFP- and hemagglutinin (HA)-tagged WT and both mutant actins into cytoplasmic stress fibers. Adenoviral vectors of HA-tagged WT and Y166C actin were successfully used to infect adult and neonatal rat cardiomyocytes (NRCs). The expressed HA-tagged actins were incorporated into the minus-ends of NRC thin filaments, demonstrating the ability to form hybrid thin filaments with endogenous actin. In NRCs, the Y166C mutant led after 72?h to a shortening of the sarcomere length when compared to NRCs infected with WT actin. Thus our data demonstrate that a mutant actin can be integrated into cardiomyocyte thin filaments and by its reduced mode of myosin interaction might be the basis for the initiation of HCM.     

Stabilization and trajectory tracking of autonomous airship's planar motion

The stabilization and trajectory tracking problems of autonomous airship＇s planar motion are studied. By defining novel configuration error and velocity error, the dynamics of error systems are derived. By applying Lyapunov stability method, the state feedback control laws are designed and the close-loop error systems are proved to be uniformly asymptotically stable by Matrosov theorem. In particular, the controller does not need knowledge on system parameters in the case of set-point stabilization, which makes the controller robust with respect to parameter uncertainty. Numerical simulations illustrate the effectiveness of the controller designed.     

基于验证集辅助的脑电信号包裹式降维

当今时代信息技术的高速发展促使人们对人机交互领域投以更多的目光,随时监测操作者脑力负荷情况并依此对操作者的任务工作量进行调整,在当下有着重要意义。有研究表明,脑电信号功率谱密度对于脑力负荷分类任务较为适用,但脑电特征维数较高,极易出现维度灾难。目前机器学习中降维方面应用最广泛的算法为主成分分析(principal component analysis, PCA),针对主成分分析在脑电信号分类上的不适应性和支持向量机(support vector machine, SVM)对特征间关系的敏感性,提出了基于PCA-SVM与逐阶枚举法的包裹式降维方法,在特征工程阶段引入固定验证集概念辅助包裹式降维,以验证集精度为指标调整特征工程方案,以此提高数据降维后的可分性。由于引入了监督学习概念,实验结果表明,基于PCA-SVM与逐阶枚举法降维过后的数据分类精度要普遍高于只依靠传统PCA的降维方式,以此为高维生物电数据降维提供了新思路。     

基于MIDAS Gen的多层钢筋混凝土框架结构倒塌数值模拟分析

汶川地震中大量钢筋混凝土(reinforced concrete, RC)框架结构破坏严重,极震区其倒塌率却远超过多层砖混结构。2022年9月以来,四川频发地震,为重新探讨倒塌机理及抗震性能,本文研究通过实地震害及多层RC框架结构破坏形式,结合数值模型并应用MIDAS Gen有限元计算方法,研究了RC框架结构的倒塌机理、地震响应分析。结果表明：框架柱的破坏与屈服是RC框架倒塌的主要因素,模拟增设填充墙,当地震烈度达到10度时,层间位移角大约1/46,降低柱的轴压比,提高柱的破坏延性,增大结构的极限抗侧能力,起到抗震第一道防线的作用,避免整体屈服破坏。     

温度和化学势的相对论变换

本文通过区分有序和无序能量,并考虑温度的统计特性,得出了溫度和化学势的相对论变换的收缩结果。     

简易氮分子激光器的研制

介绍了一种简易氮分子激光器的构造；实验研究了火花隙距离与放电电压的关系和激光器输出能量与放电电压的关系．     

在拓扑空间T上一致空间X的纤维正则性和正规性

在拓扑空间T上一致空间X的定义基础上,我们在本文讨论了这个空间的正则性和正规性,并给出几个这个空间的纤维正则性和纤维正规性的判别方法.     

结构动内力的精确算法──局部效应及振型迭加法的修正

在使用集中质量法进行地震响应分析时,忽略了连续质量产生的局部效应．本文在文献*的基础上提出了一个计算局部效应的简便而合理的方法。并通过抗震计算实例说明局部效应的影响是不能忽略。     

杂交早稻米质性状遗传的初步研究

测定武昌夏季和广西秋季栽培的杂交早稻新组成威伐242F1、F2，汕优242F、F2，威20B，珍汕97B和湖大242的糙米率、精率、米粒长、米粒宽、粒形（长／宽）、垩白等级和碱消值。分析以上性状的F2变异，估算垩白等级和碱消值的广义遗传力分别为52．26％和80．40％。同时研究了异地不同季别栽培条件对米质性状的影响。     

Overture记谱软件的使用技巧

介绍了如何使用Overture记谱软件完成五线谱的输入和打印，同时介绍了怎样将五线谱和WORD文本进行混排。使五线谱文件顺利插入文本文档中。