细胞凋亡的三种形态学检测法的应用比较

文章比较分析了光学显微镜、荧光显微镜和扫描电子显微镜检测细胞凋亡的差异,探讨三种形态学检测法在尼古丁诱导的血管平滑肌细胞(vascular smooth muscle cells,VSMCs)凋亡中的应用。用终浓度为100μM的尼古丁作用于体外培养的大鼠血管平滑肌细胞24 h,不加尼古丁的细胞作为对照组,应用普通光学显微镜、荧光显微镜和扫描电子显微镜分别观察各组细胞的形态,确定尼古丁对血管平滑肌细胞凋亡的影响。三种形态学检测方法均可检测到尼古丁明显诱导血管平滑肌细胞的凋亡。荧光显微镜检测法因操作简便、灵敏度较高、价格适中,更适用于本实验。     

充填开采控制地表沉陷的空隙量守恒理论及应用研究

充填开采是解决煤矿开采造成的环境问题,控制地表沉陷的有效技术途径之一.通过分析地表沉陷的形成过程及控制机理,提出了充填开采控制地表沉陷的空隙量守恒理论,推导出了地表沉陷盆地体积计算公式.依据该理论进一步提出了控制地表沉陷的技术途径,包括提高采空区的充填率、提高充填料浆及充填体的物理力学性能及增大上覆岩层滞留空隙体积等.工程实践表明,充填开采控制地表沉陷的空隙量守恒理论在煤矿膏体充填开采实践中得到了成功应用.     

Screening and denitrification characteristics of a slight halophilic heterotrophic nitrobacteria

A slight halophilic heterotrophic nitrobacteria named gs1 was separated from the matured activated sludge. According to the morphological observation,physiological biochemical tests and sequence analysis of the 16S rDNA,strain gs1 was identified to be as Pseudomonas sp. Sodium acetate and ammonium chloride were used as carbon and nitrogen sources,respectively,to investi-gate the characteristics of the bacterium. When cultured for 24 h under aerobic conditions,with the removal rates of the NH4+-N and COD being 82.2% and 74.73%,respectively,strain gs1 will have a nitrification function of producing NO2--N. When cultured for 24 h under aerobic conditions in nitrite medium,the removal rate of the NO2--N became 100%,and when cultured for 24 h under aerobic conditions in nitrate medium,the removal rate of the NO3--N became 97%. The result shows that this strain functions for either nitrification or denitrification,i.e.,it can complete the full process of biological deoxidation.     

New method for passive radar seeker to antagonize non-coherent radar decoy

Using super resolution direction of arrival (DOA) estimation algorithm to reduce the resolution angle is an effective method for passive radar seeker (PRS) to antagonize non-coherent radar decoy. Considering the power and correlation property between radar and non-coherent decoy, an improved subspace DOA estimation method based on traditional subspace algorithm is proposed. Because this new method uses the invariance property of noise subspace, compared with traditional MUSIC algorithm, it shows not only better resolution in condition of closely spaced sources, but also superior performance in case of different power or partially correlated sources. Using this new method, PRS can distinguish radar and non-coherent decoy with good performance. Both the simulation result and the experimental data confirm the performance of the method.     

2008年日全食观测到的太阳闪耀线偏振光谱

在2008年8月1日发生在中国的日全食食既时,我们测量到了从502.8—528.5nm波段范围内的闪耀光谱的线偏振信号。我们发现了一大组光谱线(特别是那些由中性铁、中性铜还有碳分子产生的谱线)相对于连续谱拥有非常高的偏振度,而线偏振光谱比闪耀光谱本身呈现出更强的结构特征。根据观测结果,我们认为相干散射、散射几何还有其它的机制在产生如此高的偏振度中共同发挥了作用。这一观测将深化我们对太阳高层大气物理状态和物理过程的认识。     

Integrating carbon-halogen bond formation into medicinal plant metabolism

Halogenation, which was once considered a rare occurrence in nature, has now been observed in many natural product biosynthetic pathways. However, only a small fraction of halogenated compounds have been isolated from terrestrial plants. Given the impact that halogenation can have on the biological activity of natural products, we reasoned that the introduction of halides into medicinal plant metabolism would provide the opportunity to rationally bioengineer a broad variety of novel plant products with altered, and perhaps improved, pharmacological properties. Here we report that chlorination biosynthetic machinery from soil bacteria can be successfully introduced into the medicinal plant Catharanthus roseus (Madagascar periwinkle). These prokaryotic halogenases function within the context of the plant cell to generate chlorinated tryptophan, which is then shuttled into monoterpene indole alkaloid metabolism to yield chlorinated alkaloids. A new functional group-a halide-is thereby introduced into the complex metabolism of C. roseus, and is incorporated in a predictable and regioselective manner onto the plant alkaloid products. Medicinal plants, despite their genetic and developmental complexity, therefore seem to be a viable platform for synthetic biology efforts.     

国产WSD690E高强钢焊接试验及工程应用

呼和浩特抽水蓄能电站输水系统压力钢管采用的WSD690E高强钢是国内钢厂开发的水电站引水压力钢管专用800MPa级高强钢,首次用于水电站压力钢管工程。经焊接性试验、焊接工艺评定和工程实际应用,证明钢板的加工性较好,焊接裂纹敏感性不高。在焊接线能量,预热、层间、后热温度适当的情况下焊接,接头的各项力学性能指标符合设计要求,焊缝质量优良。     

复合电源蓄能修井机能量管理策略

针对网电修井机在使用时由于井场供电限制导致的变压器过载、井场短时停电以及下放作业能量浪费等问题,提出了以复合电源作为电动修井机的蓄能器,对修井机的输出功率进行补偿,并针对修井作业工况的特殊性,设计了一种基于局部优化的逻辑门限控制策略。利用MATLAB/Simulink建立主要部件仿真模型并对典型工况进行仿真。仿真结果表明,所设计控制策略能够对各动力源的能量进行合理分配,超级电容的高利用率能够更加充分地发挥其优势,降低蓄电池能量流通与功率输出幅值,延长了蓄电池使用寿命。     

甘肃某公路隧道二衬渗漏水处置措施研究

隧道渗漏水是一种常见的病害,如果不进行有效的治理,将严重影响隧道的正常运营．通过对甘肃某隧道进口浅埋段的渗水情况及周边水文地质环境进行调查分析,认为当地特殊的地质条件、隧道施工工序和防排水措施是导致其渗漏水的主要原因．结合现有的渗漏水治理技术与现场实际情况,探索出以下治理措施：对于渗水量小的点状渗水采取以堵为原则,运用凿洞堵漏治理;渗水量较大的线状渗水采取以排为原则,运用凿槽引排治理;渗水量严重的施工缝采取堵排结合的原则,运用围岩和衬砌注浆与凿槽引排治理．实践表明治理效果较好,对公路隧道二衬渗漏水的防治具有一定的参考价值．     

短时延网络控制系统的状态反馈控制器设计

研究了短时延网络在网络时延、丢包和数据包时序错乱的网络环境下,网络控制系统(NCSs)的建模、稳定性分析、状态反馈增益设计以及控制器参数优化等问题.在稳定性分析中,获得了系统的稳定性充分条件.采用锥互补线性化(CCL)算法求解了镇定控制器的状态反馈增益值.将状态反馈控制器的设计问题转化为具有稳定域约束的优化问题,然后利用分布估计算法(EDA)求出其最优解,最终得到了系统控制参数的优化值.仿真结果表明,所提出的NCSs控制器设计方法可以有效保证控制系统的性能,经过优化后,控制性能得到明显改善.