Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

关于M(x)=o(x)的一个初等证明

本文的主要结果为:设μ(n)是M?bius函数,x>0为实数,若M(x)=■,则M(x)=o(x),x→∞.完成了该定理的初等证明.     

消交叉极化偏置双镜天线的分析和设计

偏置双镜天线,由于其结构不对称,交叉极化较难控制。成形时,涉及到一个非线性偏微分方程组,其精确解对任何预先给定的主镜口径场分布和馈源场分布不都存在。为此,本文提出了一个处理这一问题的近似方法,不仅提高了精度,还消除了天线的交叉极化分量。按此方法所设计的一个3米天线在12GHz 时的效率(计算值)为82%。     

固定支付利率的抵押贷款定价理论——限于在支付日提前支付或违约

建立限于在支付日提前支付或违约的固定支付利率抵押贷款的定价问题的数学模型,通过沿着利率特征线方向差分偏微分方程,建立模型的离散计算方法,同时根据一些计算实例所得的数字结果,分析和讨论它的风险特性与风险管理方法.     

慢性萎缩性胃炎与正常胃活检组织RNA分布的研究

采用异硫氰酸荧光素（FITC）标记的核糖核酸酶（FITC－RNase）作为探针，结合半薄切片技术，观察了慢性萎缩性胃炎与正常胃活检组织中RNA的分布变化，结果表明：慢性萎缩性胃啖腺体细胞内RNA明显减少，分布部位与正常胃腺体细胞差异较大，这些结果于慢性萎缩性胃炎的病理诊断提供了一些依据，对进一步从分子水平上研究慢性萎缩性胃炎的病理变化及其与胃癌的关系具有一定的意义。     

Au吸附在GaAs（110表面的研究

研究了Au吸附在GaAs(110)表面的吸附构型,并计算了表面态密度和表面能带结构.计算结果表明,可以认定Au与Ga的悬挂键杂化成键,从而引起2条附加的能带,一条位于禁带之中,距价带顶为0.9ev;另一条与价带共振,离价带顶为0.7ev,所得结果与实验结果相一致.     

固体吸附制冷系统发生器温度场数值模拟

以内燃机排气－固体吸附制冷系统为背景，建立了固体吸附制冷系统发生器瞬态温度场数值计算模型，并讨论模型中涉及的部分物性参数。通过实验验证，该模型能较好地模拟发生器内瞬态温度场。     

城市规划辅助审批系统中的相似度研究

城市规划的审批是一件难于量化的工作，它受政治经济等多方面因素影响，是城市规划领域关注的一大焦点．本文尝试将CBR技术用于城市规划辅助审批，让政府的审批依据达到历史与现实的统一．实现CBR的一个关键是相似度的设计，本文结合城市规划辅助审批领域的特点，设计了可行的方案，并应用于实际的办公系统中．     

Preparation of organic thin-film field effect transistor

The organic thin-film field effect transistor was prepared through vacuum deposition by using teflon as di-electric material. Indium-tin-oxide acted as the source and drain electrodes. Copper phthalocyanine and teflon were used as the semiconductor layer and dielectric layer, respectively. The gate electrode was made of Ag. The channel length between the source and drain was 50 μm. After preparing the source and drain electrodes by lithography, the copper phthalocyanine layer, teflon layer and Ag layerwere prepared by vacuum deposition sequentially. The field effect electron mobility of the device reached 1.1×10ˉ6 cm2/(V@s), and the on/off current ratio reached 500.