IGF-ⅡP3脱氧核酶活性的实验研究

目的 利用肝癌细胞株SMMC-7721 比较IGF-ⅡP3脱氧核酶的活性.方法 噻唑蓝比色法(MTT)检测4种脱氧核酶在不同浓度、不同作用时间、不同给予方法时的催化活性.结果 DRz1的抑制活性相对较高(P<0.05);脱氧核酶对靶基因随浓度增加抑制率相应提高,呈现一定的浓度依赖性;脱氧核酶24h的抑制率明显高于其他实验组(P<0.05);转染给予法显著优于直接给予法(P<0.05).结论 脱氧核酶对靶基因的抑制率呈浓度、时间依赖性.     

Evidence for metasomatic mantle carbonatitic magma extrusion in Mesoproterozoic ore-hosting dolomite rocks in the middle Kunyang rift, central Yunnan, China

The Kunyang rift lying on the western margin of the Yangtze platform is a rare Precambrian Fe-Cu mineralization zone. Wuding- Lufeng basin that is an important part of the zone is located on the west edge in the middle of the rift. The most important ore-hosting rocks are Mesoproterozoic dolomite rocks in the basin controlled by a ring fracture system, which is a fundamental structure of the basin. Plenty of silicate minerals and acicular apatite, feldspar phenocrysts and small vesicular, flown line and flown plane structures, melt inclusion and high temperature fluid inclusion found in most ore-hosting dolomites suggest that this kind of rocks could not be sedimentary dolomite, marble or hydrothermal carbonate rocks. The Zr/Hf and Nb/Ta values of the rocks are identical with those of associated mantle-derived rocks, and vary widely. For the monomineral dolomite, δ^18OSMOW‰=＋5.99 to ＋18.4 and δ^13CPDB‰ = -3.01 to ＋0.94, which fall within the range for all carbonatitic volcanic rocks of the world. As for the accessory minerals, the values of δ^18OSMOW‰ of magnetite （=＋3.47 to ＋5.99‰） are close to that of the mantle （〈5.7%）, and the δ^34S‰ values of sulfides （-5.09 to ＋5.78, averaging ＋1.50） are close to that of meteorite. For all the ore-bearing dolomite rocks, εNd = ＋0.19 to ＋2.27, and the calculated Isr=0.699143, while for the associated mantle-derived rocks, εnd = ＋3.18 to ＋3.72. All the data suggest that the mineral assemblage is not only igneous but also of metasomatic mantle origin. And the presence of acicular apatite indicates that the rocks were formed by magma rapidly cooling. And the phenocryst texture and vesicular, flown and ropy and pyroclastic structures suggest that the igneous rocks were extrusive. Therefore, the ore-bearing dolomite rocks are carbonatitic volcanic rocks. This conclusion implies that most iron and copper ore deposits hosted in the dolomite rocks should be of the carbonatite type.     

课堂教学质量的区间值模糊综合评判

在分析了传统课堂教学质量评价方法之不足的基础上,设计了供教师和学生用的评价指标体系,改进了有效分的获取方法,用模糊综合评判方法,确定了教师课堂教学质量的优劣等级,利用区间数的排序方法,排列出了参评教师的的成绩名次．     

多样化教学提高植物形态解剖学教学效果

针对植物形态解剖学的特点及教学中面临的问题，采取了重点内容教师理论课上精讲、直观性内容实验课上讲、简单内容学生讲、非重点内容自学等多样化的教学方式；写小论文、实验课考试、理论课考试相结合的多样性的考试方式，提高了教学效果。     

二级公路汽车客运站建筑设计

通过两座二级公路汽车客运站的设计总结，对客运站的设计趋势作初步探讨重点叙述客运站流线组织的基本原则和平面布局设计的基本思路     

关于高观点下初等数学教育的探讨

从面高师数学教育专业的培养目标出发，就如何运用数学思想、数学方法以及现代数学工具来指导初等教学类课程的教学提出几点看法。     

低温Al2O3悬浮电解新工艺的研究

A new process of low temperature Al2O3 suspension electrolysis for produc-ing aluminum in low melting point fluo-chloride system was investigated. Current efficiency (η) expres-sion deduced from experiments on 20～80A electrolysis cell showed that main effects on η are the cell current and the surface of aluminum produced．Increasing cell current and diminishing aluminum surface can both make η increase. On an electrolysis cell of about 100A capacity with the fine γγ-Al2O3 (media diam.1.22μm) and the mixture in which F－/C1－molar ratio was 1 at 750℃，aluminum metal was obtained with 86.3% current efficiency and 11.73kWh specific energy consumption.     

华北地区无尾两栖类的复殖吸虫(吸虫纲:复殖目)

调查华北地区无尾两栖类复殖吸虫，获６ 科１１ 种，其中包括一个新种和两个宿主新记录．脐宫科的华北长囊吸虫，新种Ｄｏｌｉｃｈｏｓａｃｃｕｓ ｈｕａｂｅｉｅｎｓｉｓｓｐ． ｎｏｖ．枝腺科２ 种，其中中华斜生吸虫Ｌｏｘｏｇｅｎｅｓ ｓｉｎｅｎｓｉｓ（ＬｉｅｔＧｕ，１９７８） Ｗａｎｇ， １９８０，寄生于金线蛙，为宿主新记录．蛇颈科的罕见蛇颈吸虫Ｇｏｒｇｏｄｅｒａ （Ｅｘｔｒｅｍ ｏｄｅｒａ）ｕｎｅｘｐｅｃｔａ ，也寄生于金线蛙，为宿主新记录，并首次见于输卵管部位．此外尚有重盘科吸虫３ 种，拟巨颈科１ 种和舐血科１ 种     

知识经济与高校产业化

高等教育在知识经济中占有重要的地位。我国的高等教育还是在计划经济思维模式下 ,把高等教育当成纯而又纯的公益事业 ,只等国家投入是行不通的。必须解放思想 ,树立教育产业化意识 ,改革教育办学体制、筹资体制和运行机制 ;放权搞活 ,开放非义务教育市场 ,充分利用各类教育资源 ,采取各种融资手段 ,扩大高等教育规模与供给。高校产业化的另一个突出问题是科技成果产业化难。为此 ,建议 :第一 ,由政府筹措 ,建立科研中试风险基金 ;第二 ,建立科研与产业对接的高速信息网     

Effects of CIITA antisense RNA on the expression of HLA class II molecules

To study the effect of the major histocompatibility complex class II (MHC II) transactivator (CIITA) antisense RNA on the expression of the human leukemia (HLA) class II molecules, 5′ end cDNA sequence of CIITA gene was cloned, and antisense RNA expression vector pcDNA-II was constructed. HeLa cells transfected with pcDNA-II and pcDNA3 were induced by IFN-γ for 3 d. The expression of HLA class II molecules on HeLa/pcDNA-II cells was significantly decreased, while it has no effect on the expression of HLA class I molecules. This result suggests that the CIITA antisense RNA can inhibit the expression of HLA class II molecules in HeLa cells. It also implies a promising approach to generate immune tolerance in graft transplantation.