Construction of a bacterial artificial chromosome library for Gossypium herbaceum var. africanum

Gossypium herbaceum var. africanum is the only wild cotton species within the cultivated diploid G. herbaceum. As the A sub-genome donor of tetraploid cotton, it is characterized by its resistance to insects, diseases, and other adversities. We have constructed the first bacterial artificial chromosome library (BAC) for G. herbaceum var. africanum. With high quality and broad coverage, this library includes 75000 clones, with an average insert size of about 115 kb and fewer than 4% empty clones. Our library is approximately five-fold the size of the A-genome (1667 Mb) and it provides 99.3% probability for isolating genes of interest or their sequences. Using nine SSR markers that are located on five different chromosomes and linked with resistance to Verticillium wilt, seven of nine could amplify the 40 superpools and got 1-14 hits. Because of its moderate wide coverage and relative large insert size, this library will be an important genomic resource for classifying and analyzing the evolution of cotton species, as well as for isolating disease-resistance genes and control elements.     

Suitability assessment of deep groundwater for drinking, irrigation and industrial purposes in Jiaozuo City, Henan Province, north China

Groundwater has been a major natural resource for human consumption in north China. It is necessary to appreciate the suitability of deep groundwater for drinking, agricultural irrigation, and industrial uses in this region. To this end, a total of 47 groundwater samples were collected from the study area; by comparing the concentrations of different hydrochemical variables with quality standard values, the variable fuzzy set (VFS) was applied to calculate the groundwater quality index (GQI) for various purposes, respectively. Afterward GQI spatial distribution maps were constructed using a geographic information system (GIS) tool to delineate spatial variations of groundwater quality. In this case study, the GQI spatial distribution maps reveal that the areas covered by "Maximum Permissible" groundwater for varying purposes (drinking, irrigation, and industrial) is 1377.2; 2354.7; and 3854.8 km2, respectively. The groundwater in the eastern part of the study area is suitable for drinking, with the southwestern region as the irrigation water source; the entire study area is acceptable for use in industrial, except the western part of Jiaozuo City. Therefore, the GQI spatial distribution maps can provide useful information for non-technical decision makers for better sustainable groundwater resources management.     

二甲基亚砜和胎牛血清对293T细胞冷冻效果的影响

使用含不同体积分数二甲基亚砜(DMSO)(5%、10%、15%、20%)和不同体积分数胎牛血清(FBS)(5%、10%、20%、40%)的细胞冷冻液以三步冷冻法冻存293T细胞.台盼蓝染色法进行活细胞计数后计算出解冻后细胞存活率.结果表明:FBS对293T细胞冷冻效果的影响是显著的(P<0.05),但添加20%和40%FBS的效果无显著差异(P>0.05);DMSO对293T细胞冷冻效果的影响则是极显著的(P<0.01),添加10%DMSO时,细胞的冷冻效果最好;冷冻液中含10%DMSO、40%FBS时的冷冻效果最佳,细胞存活率可达到82.00%.而实际应用中,含10%FBS、10%DMSO或20%FBS、5%～10%DMSO的细胞冷冻液也可达到较理想的冷冻效果,足以达到一般实验的要求.     

篌竹笋化学保藏工艺条件研究

对篌竹笋化学保藏工艺条件进行研究和探讨 ,采用氯化钠、山梨酸钾、苯钾酸钠、柠檬酸、抗坏血酸配制联合试剂 ,其保藏效果明显 ,不仅解决了篌竹笋的褐变问题 ,而且可使产品保质 4个月以上。     

引力子三点与四点Green函数对量子Wilson圈的贡献

基于微扰法 ,计算了引力子三点、四点Green函数对Einstein引力的量子Wilson圈的贡献 .结果表明 ,在κ4 阶Einstein引力又获得了定域曲率的激发     

海东地区森林资源管护工作现状及对策

经对海东地区森林资源管护工作的调查,针对存在的问题和现状.提出搞好森林资源管护工作的对策.     

一种新非简谐振子势的阶梯算符

本文运用超对称构造出了一种新精确可解非简谐振子势的能量阶梯算符，并由阶梯算符求出了其能量本征值和本征函数。     

Evaluation of genetic diversity and relationships within and between two breeds of duck based on microsatellite markers

The genetic diversity of two natural populations (M, N) of Beijing duck (Anas platyrhynchos) and 11 artificially selected lines of Beijing duck (A, B, E-L, O) from China Gold Star Duck Production Ltd., along with two Cherry Valley duck lines (C and D) from the British Cherry Valley Livestock Division, was evaluated using 18 microsatellite markers covering 16 linkage groups. A phylogenetic tree of the 15 populations of duck, formed of four main branches, was constructed from Nei’s D_A genetic distance. The mean genetic differentiation index (F_ST) in all loci, Nei’s standard genetic distance (Ds), and the genetic distance D_A between the Beijing duck and the Cherry Valley duck were 0.075, 0.143 and 0.142, respectively. These results demonstrated a high degree of genetic similarity between the two breeds and supported the hypothesis that the Cherry Valley duck was derived from the Beijing duck. The F_ST matrix of seven clusters of Beijing duck suggested that the efficiency of selection was not significant to some extent and should be supplemented by marker-assisted selection.     

A Sensitivity-Based Heuristic Search for Constrained Optimization in Complex Systems

1.INTRODUCTIONThealgorithmforconstrainedoptimizationincomplexsystemscanbedividedintotwoclasses:exactandheuristicmethods.Theexactmethods,whicharepartlysummarizedbyTitanetal.[1],havecomputationalrequiremeatsthatgrowexponentiajlywiththesizeoftheproblem.AlthoughheuristicmethodshavemodestcomputationalrequirementsandareeasytoAnplemellt,theyaregenerallyforredundancyallocation[2-4].2.S~EMENTOFProBLEM2.1NotationN:nUmberofcomponents;M:numberofconstraints;Rs:systemreliability;Rj:thereliabili…     

薏苡仁酯对荷瘤小鼠红细胞免疫功能及Na~+,K~+-ATPase活性的影响

薏苡仁酯对荷瘤小鼠红细胞免疫功能具有显著的促进作用，对红细胞膜上的Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ有抑制作用。其抑制作用呈量效正相关。薏苡仁酯的后一作用可能是其促进荷瘤小鼠红细胞免疫功能的机理之一。