A genome-wide association study in Han Chinese identifies new susceptibility loci for ankylosing spondylitis

To identify susceptibility loci for ankylosing spondylitis, we performed a two-stage genome-wide association study in Han Chinese. In the discovery stage, we analyzed 1,356,350 autosomal SNPs in 1,837 individuals with ankylosing spondylitis and 4,231 controls; in the validation stage, we analyzed 30 suggestive SNPs in an additional 2,100 affected individuals and 3,496 controls. We identified two new susceptibility loci between EDIL3 and HAPLN1 at 5q14.3 (rs4552569; P = 8.77 × 10(-10)) and within ANO6 at 12q12 (rs17095830; P = 1.63 × 10(-8)). We also confirmed previously reported associations in Europeans within the major histocompatibility complex (MHC) region (top SNP, rs13202464; P < 5 × 10(-324)) and at 2p15 (rs10865331; P = 1.98 × 10(-8)). We show that rs13202464 within the MHC region mainly represents the risk effect of HLA-B*27 variants (including HLA-B*2704, HLA-B*2705 and HLA-B*2715) in Chinese. The two newly discovered loci implicate genes related to bone formation and cartilage development, suggesting their potential involvement in the etiology of ankylosing spondylitis.     

Cdk5 targets active Src for ubiquitin-dependent degradation by phosphorylating Src(S75)

The non-receptor tyrosine kinase Src is a critical regulator of cytoskeletal contraction, cell adhesion, and migration. In normal cells, Src activity is stringently controlled by Csk-dependent phosphorylation of Src(Y530), and by Cullin-5-dependent ubiquitinylation, which affects active Src(pY419) exclusively, leading to its degradation by the proteosome. Previous work has shown that Src activity is also limited by Cdk5, a proline-directed kinase, which has been shown to phosphorylate Src(S75). Here we show that this phosphorylation promotes the ubiquitin-dependent degradation of Src, thus restricting the availability of active Src. We demonstrate that Src(S75) phosphorylation occurs in vivo in epithelial cells, and like ubiquitinylation, is associated only with active Src. Preventing Cdk5-dependent phosphorylation of Src(S75), by site-specific mutation of S75 or by Cdk5 inhibition or suppression, increases Src(Y419) phosphorylation and kinase activity, resulting in Src-dependent cytoskeletal changes. In transfected cells, ubiquitinylation of Src(S75A) is about 35% that of wild-type Src-V5, and its half-life is approximately 2.5-fold greater. Cdk5 suppression leads to a comparable decrease in the ubiquitinylation of endogenous Src and a similar increase in Src stability. Together, these findings demonstrate that Cdk5-dependent phosphorylation of Src(S75) is a physiologically significant mechanism of regulating intracellular Src activity.     

基于产品基因的无人探测小车概念设计方法

为解决概念设计阶段由于设计约束和关键信息模糊不全,导致设计者在进行设计时具有一定的盲目性,且设计出的方案可行性难以保证的问题,提出一种基于产品基因的概念设计方法,创建了概念设计过程模型.在此基础上,提出了产品基因的概念和结构及一种基于功能元的产品基因编码方法.以无人探测小车为例对基于产品基因的概念设计方法进行了说明.结果表明该方法能够使影响概念设计的关键产品信息明确化,降低设计的盲目性,从而提高设计方案的可行性和设计效率.      

通用试题库的研究与开发

介绍了通用试题库的设计与实现,利用这种方法开发的试题库可以实现考试工作的现代化管理。     

可变抽样区间的HL控制图

研究了基于VSI的Hodges-Lehmann控制图,与原来的FSI Hodges-Lehmann控制图相比,效率上有极大的提高.     

基于效益最大化的桥梁检修决策模型

运用贝叶斯分析方法,建立一种确定桥梁检修策略的动态模型,该模型包含了多阶段的"信息收集--数据分析--决策"过程,同时将先验信息和检查信息相结合,为决策提供更加充分的数据支持.算例考虑了两种检查方案和4种维修方案集,以检查净效益最大化为目的,在分析基准期内设计最佳的检查时间和检查方案,然后以维修综合成本最小为原则对可能出现的检查结果制定最佳维修方案.     

自蔓延高温合成及其复合材料新工艺的研究

由自蔓延高温合成技术制取的材料普遍具有孔隙率大的特点，而把ＳＨＳ工艺和熔融金属渗入技术结合可以制得致密度高的复合材料，复合材料性能测试结果表明，硬度提高了４４ＨＢ，相对耐磨性提高了８２％。     

一类带参算子的性质及应用

本文讨论积—微分方程研究中经常涉及的一类积分算子性质,并给这了若干应用的例子。     

在水运系统中用排队理论优选船舶与泊位元素

本文论述了一种以排队理论结合蒙特卡洛试验分析随机模拟船舶营运,以秦皇岛至上海航线上的煤炭运输为例进行水运系统分析的方法.分析中的变量参数有:船型尺度,泊位规模与个数,装卸机械的型式和装卸率.按实际情况分析,确定了船舶到达的分布函数和装卸服务时间的分布函数,以适当的经济指标为目标函数,对船舶、装卸机械和泊位的组合情况进行了计算.可以看出在此水运系统中泊位投资相对船舶投资比例较小,若采用低速和较小长宽比的船舶并配有高效的连续式装卸机将会有较好的经济效益.     

山楂苗木根系药剂处理的生理效应

用α—萘胺法、纸层析法和比色法对NAA处理的山楂苗木根系活力、氨基酸种类和含量分别进行了测定;同时用垂直板型聚丙烯酰胺凝胶电泳法对山楂叶和根内的过氧化物同工酶的酶谱进行了分析。结果表明,NAA处理能增加根内氨基酸种类和含量,也提高了根系活力。10mg·kg~(-1)NAA处理对根和叶内过氧化物同工酶的酶带和酶量均有影响,而5mg·kg~(-1)处理的与对照类似。