Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

When used in combination with certain chemotherapies, curcumin has been shown to increase apoptosis in several cancer cell lines. Here, we report the combined effects of curcumin and cinobufacini on human cervical carcinoma cells. The aim of this study was to examine whether curcumin could enhance apoptosis induced by cinobufacini. 3-(4,5-Dimethylthiazol-2-y1)-2,5- diphenytetrazolium bromide (MTT) assays revealed that the growth and proliferation of HeLa cells could be inhibited by 75% after a combined treatment of 25 μg/mL cinobufacini and 8 μg/mL curcumin. The combined treatment is 3 times more effective than treatment with 25 μg/mL cinobufacini alone. Annexin V-FITC/PI staining, morphological changes and immunofluorescence verified a significant enhancement in cinobufacini-induced apoptosis when cells were also exposed to curcumin. The data showed that the proportion of early apoptotic cells significantly increased from 15.43% in cells treated only with 25 μg/mL cinobufacini to 49.2% in cells treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. Moreover, compared with treatment of only 25 μg/mL cinobufacini, ROS production increased 1.7-fold, the intracellular free Ca 2+ concentration increased 1.5-fold, and the mitochon- drial membrane potential decreased by 20% in the combined treatment. Simultaneously, the atomic force microscopy (AFM) re- sults suggest that cells treated with a combination of cinobufacini and curcumin varied significantly in shape and ultrastructure. Collapsed cells with leaking cytoplasm, blebbing pores and emerging apoptotic bodies were prevalent. The nanoparticle size increased from 70 nm when the cells were treated with 25 μg/mL cinobufacini to 190 nm when the cells were treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. The size increase resulted in the cell membrane becoming considerably rough. These results can improve our understanding of combination treatments. Specifically, the combination of cinobufacini and curcumin may potentially find use as a novel cervical carcinoma treatment. Additionally, AFM is a powerful tool that can be used to explore cellular morphologies and ultrastructures.     

密云水库沉积物中重金属元素分布及形态研究

主要研究了北京市密云水库沉积物中重金属的总量以及形态分布特征,采用风险评价准则法(RAC)和Tucker3模型探讨沉积物中重金属的潜在危害性.结果表明,各重金属元素的平均含量都超过北京市土壤背景值,重金属元素有一定的富集.Cu有机结合态约占总量的3%～68%;Mn可交换态、碳酸盐结合态约占总量的1%～18%、7%～31%;Pb、Zn的铁锰氧化物结合态分别占总量的12%～53%、4%～27%;其它重金属均以铁锰氧化物结合态和残渣态为主,平均含量占总量的80%以上.根据RAC法得出,Mn(除采样点18)处于中等风险甚至高风险等级;Pb、Zn、Cu属从低风险到中等风险级;Ni、V、Cr、Ti的潜在生态风险较低.该结论将为北京市密云水库保护、人体健康安全保护措施的制定提供科学依据.     

民族高校图书馆音视频民族文献资源建设初探

简述了民族高校图书馆音视频民族文献资源建设的重要性,指出了音视频民族文献资源建设存在的问题,提出了音视频民族文献资源建设的策略。     

茶叶主要组分与发酵度联系的研究

利用傅里叶红外光谱和特征基理论,对茶叶主要组分与茶叶发酵度的联系进行了研究.采集茶叶的主要内含组分的中红外光谱图,建立茶叶单质光谱特征基.通过改变建基的单质种类,根据不同发酵程度茶叶的分布情况,并对比分析得出4种儿茶素和3种氧化物是影响重度发酵茶发酵程度的主要因素,叶绿素是中度发酵茶和轻度发酵茶发酵程度不同的主要因素.     

基于数据挖掘的网络业务流分析方法

为了从业务角度对网络的性能进行评价和优化,提出了一种新的网络业务分析方法——具有时态路径约束的关联规则挖掘分析方法.该方法以网络业务为分析粒度,以与网络业务流相关的时态属性和路径属性为约束条件,对已经积累的反映网络状况的海量历史数据进行挖掘分析.在进行关联规则挖掘时,利用频繁数据项集的性质,通过引入事务标号,在求出候选频繁项集的同时也求出其支持度,避免了为求支持度而进行的扫描数据库运算,极大提高了挖掘的效率和速度.实验结果表明,进行挖掘分析的数据量越大,该方法的性能和效率就越好.     

Shape demagnetization effect on layered magnetoelectric composites

Magnetoelectric （ME） voltage coefficient of plate layered magnetostricUve-piezoelectric composites was always considered independent of the in-plane size due to the oversimplification in ME theoretical models. Here we report that the ME voltage coefficient depends on the in-plane size owing to shape demagnetization effect on magnetostriction. Both theoretical analysis and experimental results indicate that ME voltage coefficient of layered ME composites changes with the variation in in-plane sizes （length and width）. ME coefficient increases with a rise in in-plane sizes, and different aspect ratio also results in different ME coefficients. Proper design of in-plane shape will greatly promote the development of ME devices.     

广西钦州配电网电容电流核实测试出现问题的分析及对策

从广西电网发生的几起配电网事故说明测试配电网电容电流的重要性,接着介绍了传统和现在测量配电网电容电流的方法并分析其优缺点,从而证明新方法在电力系统的适用性、可行性,并从钦州供电局测试的数据进行分析,指出现阶段测试配电网电容电流的关键及注意事项.     

一种用于异常检测的网络流量抽样方法

为了减小抽样数据对网络异常检测的影响,提出了一种新的可变抽样率的网络流量抽样方法.通过利用哈希模式匹配算法,将到达的数据报文按流标识分类并记录下该报文在流中的位置,然后根据报文所属流的位置顺序减函数来设置不同的报文抽样概率.实验结果表明,所提方法增加了短流报文的抽样概率,解决了由于随机报文抽样方法偏向于长流抽样而导致的网络异常丢弃的问题,从而提高了异常检测的正确性.     

我国体育赛事电视转播权经营开发的影响因素探析

从体育、广电小系统到整个社会大系统,审视了影响国内体育赛事电视转播权经营开发的主、客观因素,科学探寻了问题背后的原因和症结,提出了有过渡性特征,有中国特色的体育赛事电视转播权经营开发的对策建议,以期为规范和推进国内赛事电视转播权的有序开发提供参考。     

图的顶点划分与图的上可嵌入性

图G的顶点W-划分是指G的一个顶点划分{V1,V2,…,Vs),其中G[Vi]有生成子图轮W[V1](1≤i≤s).结合图的顶点W-划分以及顶点度条件,得到了一类新的上可嵌入图类,推广了已有相关结果.