Genomic organization of EDSV strain AA-2

The genomic organization of egg drop syndrome virus strain AA-2 isolated in China is reported here. The genome was found to be 32 838 bp in length, approximately 2 kb shorter than those of the human subgenus C adenoviruses. Analysis of open reading frames indicated that the genes for major viral structural proteins (55 K, Penton base, pⅦ, pⅩ, pⅥ, Hexon, 100 K, pⅧ and Fiber), as well as EP, DNA polymerase and ⅣaⅡ are present in the expected locations in the genome. EDSV possesses no identifiable E3 and E4 regions and most proteins encoded by E3 and E4 regions of other adenoviruses genome were not presented in EDSV.     

A continuous 200-ka palaeoclimatic record from stalagmite in Tangshan Cave, Nanjing

An oxygen isotope temperature record from 381 to 166 kaB. P. (²³⁰Th/²³⁴U dating and tuned ages) has been obtained by combining data on the isotopic composition of calcites with that of fluid inclusions trapped in a stalagmite from a limestone cave in which a fossil cranium deposited. Several laminae in microsequence of the stalagmite represent climatic events or shifts. The Nanjing man fossil was older than 381 kaB. P.     

High-precision timing of the Quaternary standard samples with thermal ionization mass spectrometry (TIMS) U-series method

Two national stalagmite standard (GBW04412, GBW04413) and one international coral standard (RKM_4) have been determined by using thermal ionization mass spectrometry (TIMS) method. The values of ( 234 U/ 238 U) act , ( 230 Th/ 234 U) act and age are all consistent in error range with the standard values obtained by using α spectrometry.     

Osmium isotope determination on mantle-derived peridotite xenoliths from Panshishan with N-TIMS

The¹⁸⁷Os/¹⁸⁸Os ratios of spinel lherzolite xenoliths from Panshishan determined with N-TIMS are lower than that of the primitive mantle, which shows depleted mantle characteristics. Their positive correlation with Al₂O₃/MgO suggests that the geochemical behavior of trace elements Re and Os is similar to that of Al₂O₃ and MgO respectively during the magmatic evolution and Re-Os isotopic system is largely immune to mantle metasomatism. A model age of 2.8–3.4 Ga obtained by¹⁸⁷Os/¹⁸⁸Os-Al₂O₃/ MgO correlation might represent the homogeneous age of the mantle lithosphere beneath the area.     

Chronology of environmental events over East Asia during the past 130 ka

In the transitional region between the Loess Plateau and deserts, 4 sections located respectively at Yuling, Hengshan, Dingbian and Huining of China are studied. The high-resolution paleoclimatic records obtained show that environmental events on varying time scales have happened over East Asia. These events are dated with the time scales established using the recently-developed approach of Automatic Orbital Tuning. This leads to the establishment of the chronology of the environmental events.     

Effects of extracellular calmodulin on pollen germination and tube growth

The effects of calmoddin (CaM) antagonist W7-agarose, anti-CaM serum and exogenous purified CaM on pollen germination and tube growth ofForsythia suspensu were studied. The pollen germination and tube growth were inhibited or completely stopped by CaM antagonist W7-agarose. The addition of exogenous purified CaM stimulated pollen germination and tube growth, whereas the same amount of bovine serum albumin (BSA) had no effect. The inhibitory effects caused by W7-agarose and anti-CaM serum could be reversed completely by the addition of exogenous purified CaM. The tube growth of germinated pollen was also inhibited or completely stopped by W7-agarose. The results suggest that endogenous extracellular CaM initiates pollen germination and tube growth, whereas exogenous CaM enhances the above processes.     

Identification and classification of subspecies of Asian cultivated rice and their hybrids

Having reviewed the major classification systems proposed by various scholars across the world, it is found thatindica andjaponica underO. sativa L. are two major directions thoroughly differentiated from the Asian cultivated rice, forming the framework of the classification structure. A system withindica andjaponica as the only two subspecies is therefore reiterated. There are various ways to determine the indica-japonica identity of hybrid rice, but the “combined morphological trait index” (CMT index) method is more efficient and easier to handle, although the isozyme analysis, molecular marker analysis and grain quality assay methods are also feasible.     

沥青砂浆粘弹性断裂扩延分析及其断裂特性的推求

以沥青砂浆材料为例,通过薄板的蠕变断裂及单轴拉伸蠕变的试验结果和数据分析,推求出沥青砂浆在不同温度下的断裂特性参数Γ和σｍＩ１。     

Cloning and expression of the vp39 gene ofBombyx mori nuclear polyhedrosis virus inE. coli

The nuclear capsid protein gene (vp39) ofBombyx mori nuclear polyhedrosis virus (BmNPV) was amplified successfully by PCR technique and inserted into pGEM 3zf(+). The 5′ and 3′ terminal area of the amplified vp39 gene were sequenced with silver-staining dideoxy method. Bmvp39 gene was sub-cloned into the expression vector pRSET-A, and transformed intoE. coli BL21. This gene was highly expressed by IPTG induction. SDS-PAGE analysis showed that the expressed protein is about 38 kd, and the expressed amount reached maxium in 4 h with IPTG induction. Supported by the National Natural science Foundation of China and the Doctoral Foundation of Edn carto Committee Liu Deli: born in 1954, Doctoral Candidate from Huazhong Normal University To whom correspondence should be addressed: (027-7882712-2938)     

科技革命与女性的辉煌

人类在进入文明社会以后,经历了三次科技革命。科学技术作为人类文化中最活跃的组成部分,不仅使世界的政治,经济领域发生了深刻的变化,而且还渗入到占人类一半但千百年来被束缚于家庭的妇女领域,对于女性的觉醒,女性的解放,女性的发展起到不可估量的作用