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排序方式: 共有468条查询结果,搜索用时 31 毫秒
221.
根据川东大池干井构造带的钻井岩心和薄片资料,从岩性特征、古生物特征入手,结合区域地质综合分析,将上石炭统划分为三个段。文章详细地研究了沉积环境及演化特点,划分出5种沉积相,并研究了它们的识别标志,及在横向上的展布规律。认为受表生期溶蚀改造的白云岩段是最好的储层段,而碳酸盐砂滩相中的颗粒云岩则是最好的储集岩。 相似文献
222.
提出了蒸发流程终点浓度智能控制算法,应用启发式最优控制方法,将系统动态过程信息用节点表示,建立了启妇式最优控制规则。该算法可据系统特征状态,控制效果及评价函数选择控制规则,修正控制输入,仿真实验证明,该算法有较好的适应性。 相似文献
223.
Summary HeLa cell DNA was analyzed through CsCl density gradient centrifugation and thermal denaturation. Neutral centrifugation without ionic treatment allowed the resolution of a main peak with density 1.699 g/ml and of 3 satellites DNAs with densities 1.683, 1.710 and 1.720. First derivatives of melting curves showed the presence of 4 DNA families, whose G+C content calculated from Tm values corresponded almost exactly to the G+C content expected from the previously densities. The extraction method seems particularly suitable for quantitative separation of DNA classes. 相似文献
224.
Marie Novak 《Cellular and molecular life sciences : CMLS》1976,32(12):1529-1530
Summary Gonadectomy of SWR of both sexes significantly reduced the number of cysticerci ofTaenia crassiceps, 60 days post infection. There was a significant decrease in the total number of larvae and the number of nonbudding individuals, corresponding with increased number of budding larvae. This indicates that the asexual multiplication of cysticerci in populations from gonadectomized mice was inhibited. 相似文献
225.
Cichon S Buervenich S Kirov G Akula N Dimitrova A Green E Schumacher J Klopp N Becker T Ohlraun S Schulze TG Tullius M Gross MM Jones L Krastev S Nikolov I Hamshere M Jones I Czerski PM Leszczynska-Rodziewicz A Kapelski P Bogaert AV Illig T Hauser J Maier W Berrettini W Byerley W Coryell W Gershon ES Kelsoe JR McInnis MG Murphy DL Nurnberger JI Reich T Scheftner W O'Donovan MC Propping P Owen MJ Rietschel M Nöthen MM McMahon FJ Craddock N 《Nature genetics》2004,36(8):783-4; author reply 784-5
226.
Tschumperlin DJ Dai G Maly IV Kikuchi T Laiho LH McVittie AK Haley KJ Lilly CM So PT Lauffenburger DA Kamm RD Drazen JM 《Nature》2004,429(6987):83-86
Physical forces elicit biochemical signalling in a diverse array of cells, tissues and organisms, helping to govern fundamental biological processes. Several hypotheses have been advanced that link physical forces to intracellular signalling pathways, but in many cases the molecular mechanisms of mechanotransduction remain elusive. Here we find that compressive stress shrinks the lateral intercellular space surrounding epithelial cells, and triggers cellular signalling via autocrine binding of epidermal growth factor family ligands to the epidermal growth factor receptor. Mathematical analysis predicts that constant rate shedding of autocrine ligands into a collapsing lateral intercellular space leads to increased local ligand concentrations that are sufficient to account for the observed receptor signalling; direct experimental comparison of signalling stimulated by compressive stress versus exogenous soluble ligand supports this prediction. These findings establish a mechanism by which mechanotransduction arises from an autocrine ligand-receptor circuit operating in a dynamically regulated extracellular volume, not requiring induction of force-dependent biochemical processes within the cell or cell membrane. 相似文献
227.
Trifunovic A Wredenberg A Falkenberg M Spelbrink JN Rovio AT Bruder CE Bohlooly-Y M Gidlöf S Oldfors A Wibom R Törnell J Jacobs HT Larsson NG 《Nature》2004,429(6990):417-423
Point mutations and deletions of mitochondrial DNA (mtDNA) accumulate in a variety of tissues during ageing in humans, monkeys and rodents. These mutations are unevenly distributed and can accumulate clonally in certain cells, causing a mosaic pattern of respiratory chain deficiency in tissues such as heart, skeletal muscle and brain. In terms of the ageing process, their possible causative effects have been intensely debated because of their low abundance and purely correlative connection with ageing. We have now addressed this question experimentally by creating homozygous knock-in mice that express a proof-reading-deficient version of PolgA, the nucleus-encoded catalytic subunit of mtDNA polymerase. Here we show that the knock-in mice develop an mtDNA mutator phenotype with a threefold to fivefold increase in the levels of point mutations, as well as increased amounts of deleted mtDNA. This increase in somatic mtDNA mutations is associated with reduced lifespan and premature onset of ageing-related phenotypes such as weight loss, reduced subcutaneous fat, alopecia (hair loss), kyphosis (curvature of the spine), osteoporosis, anaemia, reduced fertility and heart enlargement. Our results thus provide a causative link between mtDNA mutations and ageing phenotypes in mammals. 相似文献
228.
AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus 总被引:1,自引:0,他引:1
Minokoshi Y Alquier T Furukawa N Kim YB Lee A Xue B Mu J Foufelle F Ferré P Birnbaum MJ Stuck BJ Kahn BB 《Nature》2004,428(6982):569-574
Obesity is an epidemic in Western society, and causes rapidly accelerating rates of type 2 diabetes and cardiovascular disease. The evolutionarily conserved serine/threonine kinase, AMP-activated protein kinase (AMPK), functions as a 'fuel gauge' to monitor cellular energy status. We investigated the potential role of AMPK in the hypothalamus in the regulation of food intake. Here we report that AMPK activity is inhibited in arcuate and paraventricular hypothalamus (PVH) by the anorexigenic hormone leptin, and in multiple hypothalamic regions by insulin, high glucose and refeeding. A melanocortin receptor agonist, a potent anorexigen, decreases AMPK activity in PVH, whereas agouti-related protein, an orexigen, increases AMPK activity. Melanocortin receptor signalling is required for leptin and refeeding effects on AMPK in PVH. Dominant negative AMPK expression in the hypothalamus is sufficient to reduce food intake and body weight, whereas constitutively active AMPK increases both. Alterations of hypothalamic AMPK activity augment changes in arcuate neuropeptide expression induced by fasting and feeding. Furthermore, inhibition of hypothalamic AMPK is necessary for leptin's effects on food intake and body weight, as constitutively active AMPK blocks these effects. Thus, hypothalamic AMPK plays a critical role in hormonal and nutrient-derived anorexigenic and orexigenic signals and in energy balance. 相似文献
229.
Diego Sbardella Grazia R. Tundo Andrea Coletta Julien Marcoux Efthymia Ioanna Koufogeorgou Chiara Ciaccio Anna M. Santoro Danilo Milardi Giuseppe Grasso Paola Cozza Marie-Pierre Bousquet-Dubouch Stefano Marini Massimo Coletta 《Cellular and molecular life sciences : CMLS》2018,75(18):3441-3456
The interaction of insulin-degrading enzyme (IDE) with the main intracellular proteasome assemblies (i.e, 30S, 26S and 20S) was analyzed by enzymatic activity, mass spectrometry and native gel electrophoresis. IDE was mainly detected in association with assemblies with at least one free 20S end and biochemical investigations suggest that IDE competes with the 19S in vitro. IDE directly binds the 20S and affects its proteolytic activities in a bimodal fashion, very similar in human and yeast 20S, inhibiting at (IDE)?≤?30 nM and activating at (IDE)?≥?30 nM. Only an activating effect is observed in a yeast mutant locked in the “open” conformation (i.e., the α-3ΔN 20S), envisaging a possible role of IDE as modulator of the 20S “open”–”closed” allosteric equilibrium. Protein–protein docking in silico proposes that the interaction between IDE and the 20S could involve the C-term helix of the 20S α-3 subunit which regulates the gate opening of the 20S. 相似文献
230.
Francesco Bellomo Anna Signorile Grazia Tamma Marianna Ranieri Francesco Emma Domenico De Rasmo 《Cellular and molecular life sciences : CMLS》2018,75(18):3411-3422
Nephropathic cystinosis (NC) is a rare disease caused by mutations in the CTNS gene encoding for cystinosin, a lysosomal transmembrane cystine/H+ symporter, which promotes the efflux of cystine from lysosomes to cytosol. NC is the most frequent cause of Fanconi syndrome (FS) in young children, the molecular basis of which is not well established. Proximal tubular cells have very high metabolic rate due to the active transport of many solutes. Not surprisingly, mitochondrial disorders are often characterized by FS. A similar mechanism may also apply to NC. Because cAMP has regulatory properties on mitochondrial function, we have analyzed cAMP levels and mitochondrial targets in CTNS?/? conditionally immortalized proximal tubular epithelial cells (ciPTEC) carrying the classical homozygous 57-kb deletion (delCTNS?/?) or with compound heterozygous loss-of-function mutations (mutCTNS?/?). Compared to wild-type cells, cystinotic cells had significantly lower mitochondrial cAMP levels (delCTNS?/? ciPTEC by 56%?±?10.5, P?<?0.0001; mutCTNS?/? by 26%?±?4.3, P?<?0.001), complex I and V activities, mitochondrial membrane potential, and SIRT3 protein levels, which were associated with increased mitochondrial fragmentation. Reduction of complex I and V activities was associated with lower expression of part of their subunits. Treatment with the non-hydrolysable cAMP analog 8-Br-cAMP restored mitochondrial potential and corrected mitochondria morphology. Treatment with cysteamine, which reduces the intra-lysosomal cystine, was able to restore mitochondrial cAMP levels, as well as most other abnormal mitochondrial findings. These observations were validated in CTNS-silenced HK-2 cells, indicating a pivotal role of mitochondrial cAMP in the proximal tubular dysfunction observed in NC. 相似文献