南四湖流域环境地质问题及其治理对策

南四湖流域的各种环境地质问题较突出，如水体污染、矿山环境地质灾害、地下水环境异常、南四湖淤积、地方病等。对流域环境地质问题的治理应综合考虑各种影响因素，建立完善的环境管理体制，增大环境治理投资比例，加强环境治理力度。     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system

The human adenovirus type 5 E1A, a tumor- suppressor gene[1], codes for two major related proteins of 243 amino acids (12S) and 289 amino acids (13S) by al-ternative splicing in two exons[2]. Studies have been shown that E1A can regulate expression of many genes and cell cycle[3]. Both in vitro and in vivo experiments indicated that E1A could induce tumor cells differentia-tion, convert tumor cells into an epithelial phenotype, in-hibit tumor cell growth and metastasis and strongly en-ha…     

A dynamic fuzzy clustering method based on genetic algorithm

A dynamic fuzzy clustering method is presented based on the genetic algorithm. By calculating the fuzzy dissimilarity between samples the essential associations among samples are modeled factually. The fuzzy dissimilarity between two samples is mapped into their Euclidean distance, that is, the high dimensional samples are mapped into the two-dimensional plane. The mapping is optimized globally by the genetic algorithm, which adjusts the coordinates of each sample, and thus the Euclidean distance, to approximate to the fuzzy dissimilarity between samples gradually. A key advantage of the proposed method is that the clustering is independent of the space distribution of input samples, which improves the flexibility and visualization. This method possesses characteristics of a faster convergence rate and more exact clustering than some typical clustering algorithms. Simulated experiments show the feasibility and availability of the proposed method.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

工艺规划中的组合优化问题

为了解决在工艺规划的诸多环节（如工步排序、机床负荷分配、切削参数选择等）普遍存在的组合优化问题，在分析传统方法缺陷的基础上，把一种先进的优化算法——模拟退火算法应用到工艺规划中，并针对加工中心上工步排序这一典型问题，建立组合优化数学模型，对该算法在工艺规划中的运用机理作了详细论述。结果表明，该算法把概率引入到启发式方法中，避免了陷入局部最优点的缺陷，在优化结果和计算时间方面都有明显优于常规方法，有效地提高了工艺规划系统的优化能力。     

人体血浆中游离脂肪酸的气相色谱测定

建立了以ＣＨ３ＯＨ－ＢＦ３甲酯化、毛细管气相色谱法测定人体血浆中游离脂肪酸的分析方法。方法简便可靠，回收率高。利用该方法测定了７例血浆中游离脂肪酸组成和含量，取得满意结果     

应用型工科专业课教学模式的研究

结合材料科学与工程类专业课程建设的内容与效果,探讨了21世纪应用型工科的专业课程所采用的教学方法,提出了“多媒体教学-读书报告-专业实验”三者结合的模式。     

DRDOS攻击及其防范

DOS攻击是一种常见、有效而简单的网络攻击技术,而且DOS攻击的手法也在不断发展,现已出现了威胁更大的DDOS和DRDOS。主要介绍了DDOS和DRDOS攻击的概念和原理,并提出了针对整个系统的防范对策。