Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

When used in combination with certain chemotherapies, curcumin has been shown to increase apoptosis in several cancer cell lines. Here, we report the combined effects of curcumin and cinobufacini on human cervical carcinoma cells. The aim of this study was to examine whether curcumin could enhance apoptosis induced by cinobufacini. 3-(4,5-Dimethylthiazol-2-y1)-2,5- diphenytetrazolium bromide (MTT) assays revealed that the growth and proliferation of HeLa cells could be inhibited by 75% after a combined treatment of 25 μg/mL cinobufacini and 8 μg/mL curcumin. The combined treatment is 3 times more effective than treatment with 25 μg/mL cinobufacini alone. Annexin V-FITC/PI staining, morphological changes and immunofluorescence verified a significant enhancement in cinobufacini-induced apoptosis when cells were also exposed to curcumin. The data showed that the proportion of early apoptotic cells significantly increased from 15.43% in cells treated only with 25 μg/mL cinobufacini to 49.2% in cells treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. Moreover, compared with treatment of only 25 μg/mL cinobufacini, ROS production increased 1.7-fold, the intracellular free Ca 2+ concentration increased 1.5-fold, and the mitochon- drial membrane potential decreased by 20% in the combined treatment. Simultaneously, the atomic force microscopy (AFM) re- sults suggest that cells treated with a combination of cinobufacini and curcumin varied significantly in shape and ultrastructure. Collapsed cells with leaking cytoplasm, blebbing pores and emerging apoptotic bodies were prevalent. The nanoparticle size increased from 70 nm when the cells were treated with 25 μg/mL cinobufacini to 190 nm when the cells were treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. The size increase resulted in the cell membrane becoming considerably rough. These results can improve our understanding of combination treatments. Specifically, the combination of cinobufacini and curcumin may potentially find use as a novel cervical carcinoma treatment. Additionally, AFM is a powerful tool that can be used to explore cellular morphologies and ultrastructures.     

一种基于VIKOR的设备优选方法

该文应用多属性决策方法,解决了企业在采购大型设备中所面临的决策问题,为企业选取设备提供了一种有效合理的决策模型与决策方法.首先,通过构建多属性模糊决策模型;其次,选取设备优选因素集;然后,采用最小方差法解决不同属性的权重问题,进一步对因素集进行分析,建立设备优选判断集;最后,使用VIKOR方法对备选设备的综合属性进行优选排序.通过与传统的加性加权求和选购方法进行比较证明了VIKOR方法的有效性与实用性.     

超低界面张力的二元驱油体系对水驱残余油启动和运移机理

针对三元复合驱含碱驱油体系引起地层黏土分散和运移、形成碱垢、导致地层渗透率下降,碱还会大幅度降低聚合物溶液的黏弹性,从而降低波及效率等问题,利用新研制的不加碱可形成超低界面张力两性表面活性剂,通过微观模型驱油实验,分析了聚丙烯酰胺/两性表面活性剂二元复合体系对残余油启动、运移的过程,研究了该二元复合体系对水驱后残余油的作用机理.研究表明:具有超低界面张力的聚表二元复合体系利用了聚合物溶液的黏弹特性和表面活性剂体系的超低界面张力特性,使采收率大幅度提高.残余油的启动主要以拉成油滴和油丝两种形式,在运移过程中,较大的油滴又形成更容易被驱替液携带的小油滴,从而使驱油效率提高.     

武夷山风景区松墨天牛的发生与分布

采用林间调查和FJ-Ma-02型引诱剂诱捕相结合的方法,研究了松墨天牛在武夷山风景区的发生与分布.结果表明,松墨天牛在武夷山风景区主要是零星分布,多集中发生为害单株濒死或枯死松树上,以及被马尾松毛虫为害后的局部林分,局部发生区虫口密度高,但总体虫口密度不高;成虫在风景区1年有2个发生高峰期,分别在5月中旬到6月上旬和6月下旬到7月中旬,两次高峰期诱捕到的成虫占总诱捕量(125头)的90.4%;诱捕结果还表明,武夷山风景区一般海拔高程在200～400 m之间,在低海拔地理条件下松墨天牛均有发生,受海拔高程的影响不显著;松墨天牛成虫捕获量与气压、温度、最高与最低温差、相对湿度以及降水等5个气象因子之间均没有显著的相关性..     

一类非线性网络控制系统的随机H∞控制

研究了一类带有丢包的非线性网络控制系统(NNCSs)的随机H∞控制问题.丢包过程采用满足伯努利分布的随机序列来描述.利用T-S模糊模型和平行分布补偿技术建立了带有随机参数的非线性网络控制系统的模型,并以线性矩阵不等式的形式给出了模糊控制器存在的充分条件;所设计的控制器使得非线性网络控制系统在均方意叉下指数稳定且具有一个改进的随机H∞性能.     

Alteration of the specificity ofPst I restriction endonuclease

The influence of factors on the substrate-specificity ofPst I restriction endonuclease has been studied with the method of electrophoresis. The results show that, the specificity ofPst I almost can not be influenced by the single alteration of the concentration of Tris·HCl, Mg²⁺ or Na⁺ in the reaction system, but it can be altered by the reduction of any two of them. The specificity can not be altered by the single alteration of pH or the replacement of Mg²⁺ with Mn²⁺. The addition of glycerol or dimethylsulphoxide (DM-SO) to the reaction system results in the relaxation of the substrate-specificity ofPst I, but dimethyl-methylformide, glycol and ethyl alcohol can not bring about the alteration ofPst I specificity. Through the method of cloning and sequencing, the nucleotides of No. 1 and 6 in the recognition sequence ofPst I have changed (1C→A or 6G→T). Used with the enzyme analysis of an artificially synthetic DNA segment containing a special sequence, the nucleotides of No. 1 and 6 have both changed (1C→A and 6G→T). The recognition sequence ofPst I is speculated to be changed from CTGCA→G to TGCA→. Foundation item: Supported by the Research Fund for the Doctoral Program of Higher Education. Biography: Zou Guo-lin (1947-), male, professor. research direction, biochemistry.     

Excel集成PHEES绘制水盐体系相图及模拟K2SO4生产工艺

利用Excel 97的VBA编程调用PHEES的单点固液相平衡计算模块,通过网格法绘制出Na+-K+-Cl--SO42--H2O的25℃和100℃下的相图和等水线图.并设计无机盐工艺模拟程序,模拟了K2SO4生产的一种两段法工艺.     

论村级建档的动力体系

农村档案建设已成为农村经济和社会发展的必然要求。目前,由于经济条件差、人员文化素质低、档案意识淡薄、管理体制不完善等因素的影响,广大农村地区的建档率还比较低。然而,在村级建档的动态发展中,在其自身演进的动力体系,即社会需要动力、素质动力、资金动力、宣传动力、激励动力、法律与标准动力。准确把握这一动力体系,可以促进村级建档目标的顺利实现。     

基于有限元数值模拟的直流电阻率激电找矿探测技术

为提高直流电阻率激电探测成果地质解释准确性,促进直流电阻率激电在找矿勘查中的应用,采用有限元数值模拟对直流电阻率激电探测中低阻高极化体、直立板状体、低阻盖层、高阻盖层、地表不均匀体、地形起伏及实测数据反演解释几个主要方面进行了对比分析。研究结果表明,对于实际勘查中的反演解释结果,根据异常特征构建地质体模型并进行有限元数值模拟,能快速判别探测目标体异常属性、区分虚假异常,提高定量解释水平及找矿勘查效率。研究结果为直流电阻率激电找矿探测定量解释提供了参考。     

多智能体涌现生成的群体行为仿真

众多实验表明,以建模仿真的方法研究复杂的群体行为是一条可行有效的途径.针对如何使所构建的群体行为模型在仿真过程中,既能表现自主、智能的个体行为,又能表现灵活、适应的整体行为,提出了一种基于智能体的综合建模方法,重点从信息获取、决策判断、运动控制三个方面对智能体模型的设计与实现进行了论述,并以实验结果证明了多智能体在交互.协调作用下涌现生成可视化群体行为仿真的科学性及合理性.