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971.
JIANG Yong MA TongHui 《科学通报(英文版)》2007,52(6):771-776
The asparagine-proline-alanine sequences (NPA motifs) are highly conserved in aquaporin water channel family. Crystallographic studies of AQP1 structure demonstrated that the two NPA motifs are in the narrow central constriction of the channel, serving to bind water molecules for selective and effi-cient water passage. To investigate the importance of the two NPA motifs in the structure, function and biogenesis of aquaporin water channels, we generated AQP1 mutations with NPA1 deletion, NPA2 de-letion and NPA1,2 double deletion. The coding sequences of the three mutated cDNAs were subcloned into the mammalian expression vector pcDNA3.1 to form expression plasmids. We established stably transfected CHO cell lines expressing these AQP1 mutants. Immunofluorescence indicated that all the three mutated AQP1 proteins are expressed normally on the plasma membrane of stably transfected CHO cells, suggesting that deletion of NPA motifs does not influence the expression and intracellular processing of AQP1. Functional analysis demonstrated that NPA1 or NPA2 deletion reduced AQP1 water permeability by 49.6% and 46.7%, respectively, while NPA1,2 double deletion had little effect on AQP1 water permeability. These results provide evidence that NPA motifs are important for water per-meation but not essential for the expression, intracellular processing and the basic structure of AQP1 water channel. 相似文献
972.
Study on crustal, lithospheric and asthenospheric thickness beneath the Qinghai-Tibet Plateau and its adjacent areas 总被引:2,自引:0,他引:2
ZHANG XueMei SUN RuoMei TENG JiWen 《科学通报(英文版)》2007,52(6):797-804
Based on the results of pure dispersions of Rayleigh wave tomography in the Qinghai-Tlbet Plateau and its adjacent areas, tsklng S wave velocities from previous linear inversion as the initial model, using the simulated annealing algorithm, a nonlinear simultaneous inversion has been carried out for S wave velocity and thickness of different layers, including the crust, the lithosphere and the asthenosphere. The results indicate: The crustal thickness shows strong correlation with geology structures sketched by the sutures and major faults. The crust is very thick in the Qinghal-Tibet Plateau, varying from 60 km to 80 kin. The Ilthospherlc thickness in the Qinghai-Tibet Plateau Is thinner (130-160 kin) than Its adjacent areas. And two blocks can be recognized, divided by an NNE strike boundary running between 90°E-92°E inside the plateau. Its asthenosphere is relatively thick, varies from 150 km to 230 kin, and the thickest area is located in the western Qiangtsng. India has a thinner crust (32-38 kin), a thicker lithosphere of 190 km and a rather thin asthenosphere of only 60 kin. Sichuan and Tarlm basins have the crust thickness less than 50 kin. Their Iithospheres are thicker than the Qinghai-Tibet Plateau, and their asthenospheres are thinner. A discussion has been made on the character and formation mechanism of the typical crust-mantle transition zone in the western Qiangtsng block. 相似文献
973.
HUANG Jian ZHANG JinChao ZHANG TianLan WANG Kui 《科学通报(英文版)》2007,52(7):890-895
The effect of icariin on the bone resorption activity of rabbit osteoclasts is assessed in vitro. Osteoclasts were isolated from Japanese white rabbits and cultured on plates with a sterilized bone slice in each well. After treatment with icariin at various concentrations, the bone resorption activity of osteoclasts was evaluated by examining pit areas, superoxide anion (·O2-) generation, size and number of actin rings and intracellular calcium concentration [Ca2 ]i. As revealed by these data, icariin elicited continuous decline of [Ca2 ]i, making actin ring constricted and ·O2- generation decreased. These events resulted in smaller and fewer pits which indicate suppressed bone resorption activity of rabbit osteoclasts by icariin. 相似文献
974.
Construction of a novel economy-climate model 总被引:1,自引:0,他引:1
CHOU JieMing DONG WenJie YE DuZheng 《科学通报(英文版)》2007,52(7):1006-1008
An attempt has been made to construct a novel economy-climate model by combining climate change research with agricultural economy research to evaluate the influence of global climate change on grain yields. The insertion of a climate change factor into the economic C-D (Cobb-Dauglas) production function model yields a novel evaluation model, which connects the climate change factor to the eco-nomic variation factor, and the performance and reasonableness of the novel evaluation model are also preliminarily simulated and verified. 相似文献
975.
WANG YongQiang YUAN SongLiu SONG YunXing LIU Li TIAN ZhaoMing LI Pai ZHOU YuanMing LI YunLong YIN ShiYan 《科学通报(英文版)》2007,52(8):1019-1023
Bulk samples with nominal composition Zn0.95Co0.05O and Zn0.92Co0.05Mn0.03O were fabricated by a solid-state reaction method at 600℃.X-ray diffraction experiment showed that the peaks of secondary phase Co3O4 with a cubic structure were visible in both samples,besides the main peaks of wurtzite structure as ZnO.Magnetization measurement indicated that doping Co alone can induce ferromag- netism in ZnO itself,while the introduction of Mn significantly enhances ferromagnetism.However, both samples showed different magnetic behavior at temperatures below 50 K.It was also noted that ferromagnetic coupling interaction was weakened due to the presence of antiferromagnetic Co3O4. 相似文献
976.
Clay minerals in surface sediments of the Pearl River drainage basin and their contribution to the South China Sea 总被引:5,自引:0,他引:5
LIU ZhiFei Christophe COLIN HUANG Wei CHEN Zhong Alain TRENTESAUX CHEN JianFang 《科学通报(英文版)》2007,52(8):1101-1111
Clay minerals have played a significant role in the study of the East Asian monsoon evolution in the South China Sea by being able to track oceanic current variations and to reveal contemporaneous paleoclimaUc changes prevailing in continental source areas. As one of the most important rivers inputting terrigenous matters to the northern South China Sea, the Pearl River was not previously paid attention to from the viewpoint of clay mineralogy. This paper presents a detailed study on clay minerals in surface sediments collected from the Pearl River drainage basin (including all three main channels, various branches, and the Lingdingyang in the estuary) by using the X-ray diffraction (XRD) method. The results indicate that the clay mineral assemblage consists dominantly of kaolinite (35%-65%), lesser abundance of chlorite (20%-35%) and illite (12%-42%), and very scare smectite occurrences (generally 〈5%). Their respective distribution does not present any obvious difference throughout the Pearl River drainage basin. However, downstream the Pearl River to the northern South China Sea, the clay mineral assemblage varies significantly: kaolinite decreases gradually, smecUte and illite increase gradually. Additionally, illite chemistry index steps down and illite crystallinity steps up. These variations indicate the contribution of major kaolinite, lesser illite and chlorite, and very scarce smecUte to the northern South China Sea from the Pearl River drainage basin. The maximum contribution of clay minerals from the Pearl River is 72% to the northern margin and only 15% to the northern slope of the South China Sea. In both glacials and interglacials, kaolinite indicates that the ability of mechanical erosion occurred in the Pearl River drainage basin. 相似文献
977.
YANG MeiYing MA PengDa LI WenMing LIU JinYing LI Liang ZHU XiaoJuan WANG XingZhi 《科学通报(英文版)》2007,52(9):1205-1211
Bacterium strain PJ3,isolated from wastewater and identified as Arthrobacter sp. bacterium based on its 16S rDNA gene,could use carbazole as the sole carbon,nitrogen and energy source. The genomic library of strain PJ3 was constructed and a positive clone JM109(pUCW402) was screened out for the expression of dioxygenase by the ability to form yellow ring-fission product. A 2,3-dihydroxybiphenyl dioxygenase(23DHBD) gene of 933 bp was found in the 3360 bp exogenous fragment of pUCW402 by GenSCAN software and BLAST analysis. The phylogenetic analysis showed that 23DHBD from strain PJ3 formed a deep branch separate from a cluster containing most known 23DHBD in GenBank. Southern hybridization confirmed for the first time that the 23DHBD gene was from the genomic DNA of Arthrobacter sp. PJ3. In order to test the gene function,recombinant bacterium BL21(pETW-8) was constructed to express 23DHBD. The expression level in BL21(pETW-8) was highest compared with the recombinant bacteria JM109(pUCW402) and strain PJ3. We observed that 23DHBD was not absolute specific. The enzyme activity was higher with 2,3-dihydroxybiphenyl as a substrate than with catechol. The substrate specificity assay suggested that 23DHBD was essential for cleavage of bi-cyclic aromatic compounds during the course of aromatic compound biodegradation in Arthrobacter sp. strain PJ3. 相似文献
978.
LIU Yuan SHAO Min 《科学通报(英文版)》2007,52(9):1274-1281
Black carbon is a by-product of incomplete combustion of carbon containing fuels. It can alter atmospheric radiation property and make adverse impacts on human health. The energy consumption in Beijing City depends largely on coal burning. Recently, Beijing City has been performing the municipal energy structure adjustment as a tool for air pollution abatement, aiming at the air quality goal for the Beijing 2008 Olympic Games. Based on Beijing energy use data in 2000, combined with emission factors of major sources of black carbon, the emission of black carbon in Beijing City is estimated to be 7.77 Gg. Coke, raw coal and biomass as non-commercial energy are the main contributors to municipal black carbon emissions. Based on Beijing energy planning in the year 2008, the emission of black carbon in 2008 will be 2.97 Gg if the contribution from biomass is not taken into account. Assuming that the black carbon emission from rural biomass in 2008 is the same as that in 2004, the biomass burning will be the largest emitter of black carbon to Beijing City in 2008. 相似文献
979.
WANG Wei ZHANG HePing WAN YuTian 《科学通报(英文版)》2007,52(9):1282-1286
Lining materials are widely used in buildings to cover walls and ceilings. Combustible linings may produce a potential high fire hazard in buildings. Once ignited, it propagates fire and accelerates the enclosure fire growth. Two types of lining materials were studied during the tests: block board and plywood. The test was conducted in an ISO 9705 room, where linings were mounted on walls without the ceiling. By changing the heat output of the burner, the ventilation, etc., the concentrations of CO2/CO of different lining materials were researched. The effect of test conditions on the production of CO2/CO of different lining materials was investigated, and useful experimental data were provided for the further development of numerical modeling to simulate enclosure fire growth lined with combustible materials. 相似文献
980.
ZHAO YuXin YI ShiHe HE Lin CHENG ZhongYu TIAN LiFeng 《科学通报(英文版)》2007,52(10):1297-1301
The interaction between shock wave and turbulence has been studied in supersonic turbulent mix layer wind tunnel. The interaction between oblique shock wave and turbulent boundary layer and the influence of large vortex in mix layer on oblique shock wave have been observed by NPLS technique. From NPLS image, not only complex flow structure is observed but also time-dependent supersonic flow visualization is realized. The mechanism of interaction between shock wave and turbulence is discussed based on high quality NPLS image. 相似文献