RAPD分子标记技术在高粱抗丝黑穗病研究中的初步应用

实验采用7050B(抗病)与TX622B(感病)杂交的F2感性个体的DNA及高粱丝黑穗病3073(抗病)、3049(感病)为试材.采用CTAB法提取高粱总DNA,用RAPD分子标记技术对其扩增的多态性及差异性图谱进行分析.实验结果如下:通过对电泳电压、电泳时间及点样量的筛选,建立并优化了RAPD-PCR琼脂糖凝胶检测系统;筛选了90个RAPD引物,其中30个引物扩增出清晰的多态性谱带,用这30个引物对3073(抗病)、3049(感病)2种试材进行抗丝黑穗病基因的初步分析,有11个引物扩增出多态性谱带.     

The complete genome of an individual by massively parallel DNA sequencing

The association of genetic variation with disease and drug response, and improvements in nucleic acid technologies, have given great optimism for the impact of 'genomic medicine'. However, the formidable size of the diploid human genome, approximately 6 gigabases, has prevented the routine application of sequencing methods to deciphering complete individual human genomes. To realize the full potential of genomics for human health, this limitation must be overcome. Here we report the DNA sequence of a diploid genome of a single individual, James D. Watson, sequenced to 7.4-fold redundancy in two months using massively parallel sequencing in picolitre-size reaction vessels. This sequence was completed in two months at approximately one-hundredth of the cost of traditional capillary electrophoresis methods. Comparison of the sequence to the reference genome led to the identification of 3.3 million single nucleotide polymorphisms, of which 10,654 cause amino-acid substitution within the coding sequence. In addition, we accurately identified small-scale (2-40,000 base pair (bp)) insertion and deletion polymorphism as well as copy number variation resulting in the large-scale gain and loss of chromosomal segments ranging from 26,000 to 1.5 million base pairs. Overall, these results agree well with recent results of sequencing of a single individual by traditional methods. However, in addition to being faster and significantly less expensive, this sequencing technology avoids the arbitrary loss of genomic sequences inherent in random shotgun sequencing by bacterial cloning because it amplifies DNA in a cell-free system. As a result, we further demonstrate the acquisition of novel human sequence, including novel genes not previously identified by traditional genomic sequencing. This is the first genome sequenced by next-generation technologies. Therefore it is a pilot for the future challenges of 'personalized genome sequencing'.     

沟眶象成虫肠道好氧及兼性厌氧菌群的研究

对沟眶象(Eucryptorrhynchus chinenis)成虫的肠道菌群进行了分离和鉴定.结果表明,葡萄球菌属(Sta phylococcus)是优势菌群.同时,还分离出了芽孢杆菌属(Bacillus)、假单胞菌属(Pseidp,pmas)、邻单胞菌属(Plesiomonas)、芽孢八叠球菌属(Sporasarcina)、奈瑟氏球菌属(Neisseria)、耶尔森氏菌属(Yersinia)、埃希氏菌属(Escherichia)、孤菌属(Vibirio)等共9个属.利用纤维素碳源培养基分离到了纤维素降解菌,它们与沟眶象营养及消化生理可能有极为密切的关系.     

Attack Simulation Model and Channel Statistics in Underwater Acoustic Sensor Networks

In recent years,underwater acoustic wireless sensor networks have been used in many areas.There have been many field trials of acoustic propagation models and statistics for shallow water conditions.However,field trials are limited environmentally and,hence,not widely accepted.Simulations of the impulse response of a shallow underwater acoustic channel allows less expensive system tests that are reproducable.This paper presents a shallow water acoustic channel model based on the actual acoustic propagation ...     

Soundness analysis of compositional model of time-based petri nets

Workflow management systems are widely used in improving the efficiency of business processes.To develop a workflow management system is the key to workflow modeling.In this paper,the compositional time net is presented to model workflow processes on the basis of the analyzing methods of time Petri nets and workflows,and to describe the static relationships and the dynamic semantics of the task of multiple workflow composition.The paper puts more emphasis on checking the soundness of composed workflow,at the same time,combinational problems of non-conditional structure of the compositional time net are introduced and analyzed.A sufficient and necessary condition for determining the soundness of compositional nets is obtained.     

GF(3)上一类几乎平衡的6阶分圆序列

具有良好自相关性的伪随机序列在信息安全等领域中有着广泛的应用.在GF(3)上构造一类周期为p的几乎平衡6阶分圆序列,利用6阶分圆数计算出该序列的自相关值,并进一步给出满足适当条件的素数p,使得这类序列的自相关值为3值.     

吲哚自由基的合成与晶体结构

以2-硝基丙烷为原料合成中间体2,3-二甲基-2,3-二羟胺基丁烷,然后与3-吲哚甲醛反应,合成了一种结构新颖的吲哚氮氧自由基2-(3′-吲哚基)-4,4,5,5-四甲基咪唑啉-3-氧化-1-氧基自由基(NITIn),其结构通过单晶X射线衍射法测定,该化合物空间群为P2(1)/n,a=1.111 4(7)nm,b=0.993 1(6)nm,c=1.287 7(8)nm,V=1.421 2(15)nm3,Z=4,Mr=272.32,F(000)=576,Dc=1.421 g/cm3.最终结构偏离因子R=0.060 1,Rw=0.135 3.最终差值电子密度的最大值和最小值分别为173 nm-3和-239 nm-3.     

扬子区页岩气和煤层气联合开发的地质优选

为了优选煤层气和页岩气联合研究与开发的有利区域,以扬子地区二叠系龙潭组及其上下部组合地层为研究对象,分析页岩气和煤层气的资源分布特点;讨论成藏地质条件及气藏聚集模式,依据龙潭组及其上下部地层组合的分布特征和埋深、页岩气和煤层气的资源潜力等条件,确定优选区域。研究认为,龙潭组在上扬子和下扬子普遍发育;上扬子滇东-黔西等区煤层气资源丰富;上扬子龙潭组及其与局部由长兴组相变而成的大隆组组合,下扬子大隆组—龙潭组—孤峰组组合均发育富含有机质泥页岩,厚度较大、分布连续,有机质含量高,母质类型好,成熟度高,具有页岩气成藏的良好地质条件和丰富的资源潜力,且存在页岩气和煤层气资源的重叠组合性;至少在上、下扬子分别形成三种和两种地层空间气藏聚集模式。上扬子川南资阳、自贡及重庆、黔西北地区和下扬子苏南句容盆地,可作为开展页岩气和煤层气联合研究与开发的优选区域。     

玉米淀粉基缓释膜生物降解性的测定

以玉米淀粉与聚乙烯醇为原料,在交联剂的作用下,制得包膜料液,用此料液给尿素涂膜,制得包膜尿素.通过生物降解实验,验证了它的环保性.结果表明,此包膜尿素缓释性能良好,且有保水性,抗结块性.此包膜材料具有成本低、可生物降解、无环境污染等特点.     

热电制冷口杯底部传热设计

为提高热电制冷口杯的加热与制冷效率,分析了热电制冷片三种布局下的传热效果,然后对口杯底部传热进行了设计.采用该设计将有利于在口杯上实现加热与制冷的双重功能.