排序方式: 共有45条查询结果,搜索用时 109 毫秒
31.
Gemoll T Roblick UJ Szymczak S Braunschweig T Becker S Igl BW Bruch HP Ziegler A Hellman U Difilippantonio MJ Ried T Jörnvall H Auer G Habermann JK 《Cellular and molecular life sciences : CMLS》2011,68(19):3261-3274
DNA aneuploidy has been identified as a prognostic factor for epithelial malignancies. Further understanding of the translation of DNA aneuploidy into protein expression will help to define novel biomarkers to improve therapies and prognosis. DNA ploidy was assessed by image cytometry. Comparison of gel-electrophoresis-based protein expression patterns of three diploid and four aneuploid colorectal cancer cell lines detected 64 ploidy-associated proteins. Proteins were identified by mass spectrometry and subjected to Ingenuity Pathway Analysis resulting in two overlapping high-ranked networks maintaining Cellular Assembly and Organization, Cell Cycle, and Cellular Growth and Proliferation. CAPZA1, TXNL1, and HDAC2 were significantly validated by Western blotting in cell lines and the latter two showed expression differences also in clinical samples using a tissue microarray of normal mucosa (n?=?19), diploid (n?=?31), and aneuploid (n?=?47) carcinomas. The results suggest that distinct protein expression patterns, affecting TXNL1 and HDAC2, distinguish aneuploid with poor prognosis from diploid colorectal cancers. 相似文献
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Extreme river floods have been a substantial natural hazard in Europe over the past centuries, and radiative effects of recent anthropogenic changes in atmospheric composition are expected to cause climate changes, especially enhancement of the hydrological cycle, leading to an increased flood risk. For the past few decades, however, observations from Europe do not show a clear increase in flood occurrence rate. Here we present longer-term records of winter and summer floods in two of the largest rivers in central Europe, the Elbe and Oder rivers. For the past 80 to 150 yr, we find a decrease in winter flood occurrence in both rivers, while summer floods show no trend, consistent with trends in extreme precipitation occurrence. The reduction in winter flood occurrence can partly be attributed to fewer events of strong freezing-following such events, breaking river ice at the end of the winter may function as a water barrier and enhance floods severely. Additionally, we detect significant long-term changes in flood occurrence rates in the sixteenth to nineteenth centuries, and conclude that reductions in river length, construction of reservoirs and deforestation have had minor effects on flood frequency. 相似文献
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Ellinor PT Lunetta KL Albert CM Glazer NL Ritchie MD Smith AV Arking DE Müller-Nurasyid M Krijthe BP Lubitz SA Bis JC Chung MK Dörr M Ozaki K Roberts JD Smith JG Pfeufer A Sinner MF Lohman K Ding J Smith NL Smith JD Rienstra M Rice KM Van Wagoner DR Magnani JW Wakili R Clauss S Rotter JI Steinbeck G Launer LJ Davies RW Borkovich M Harris TB Lin H Völker U Völzke H Milan DJ Hofman A Boerwinkle E Chen LY Soliman EZ Voight BF Li G Chakravarti A Kubo M Tedrow UB Rose LM Ridker PM Conen D Tsunoda T 《Nature genetics》2012,44(6):670-675
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den Hollander AI Koenekoop RK Mohamed MD Arts HH Boldt K Towns KV Sedmak T Beer M Nagel-Wolfrum K McKibbin M Dharmaraj S Lopez I Ivings L Williams GA Springell K Woods CG Jafri H Rashid Y Strom TM van der Zwaag B Gosens I Kersten FF van Wijk E Veltman JA Zonneveld MN van Beersum SE Maumenee IH Wolfrum U Cheetham ME Ueffing M Cremers FP Inglehearn CF Roepman R 《Nature genetics》2007,39(7):889-895
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Rolles D Braune M Cvejanović S Gessner O Hentges R Korica S Langer B Lischke T Prümper G Reinköster A Viefhaus J Zimmermann B McKoy V Becker U 《Nature》2005,437(7059):711-715
Because of inversion symmetry and particle exchange, all constituents of homonuclear diatomic molecules are in a quantum mechanically non-local coherent state; this includes the nuclei and deep-lying core electrons. Hence, the molecular photoemission can be regarded as a natural double-slit experiment: coherent electron emission originates from two identical sites, and should give rise to characteristic interference patterns. However, the quantum coherence is obscured if the two possible symmetry states of the electronic wavefunction ('gerade' and 'ungerade') are degenerate; the sum of the two exactly resembles the distinguishable, incoherent emission from two localized core sites. Here we observe the coherence of core electrons in N(2) through a direct measurement of the interference exhibited in their emission. We also explore the gradual transition to a symmetry-broken system of localized electrons by comparing different isotope-substituted species--a phenomenon analogous to the acquisition of partial 'which-way' information in macroscopic double-slit experiments. 相似文献
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Hennies HC Kornak U Zhang H Egerer J Zhang X Seifert W Kühnisch J Budde B Nätebus M Brancati F Wilcox WR Müller D Kaplan PB Rajab A Zampino G Fodale V Dallapiccola B Newman W Metcalfe K Clayton-Smith J Tassabehji M Steinmann B Barr FA Nürnberg P Wieacker P Mundlos S 《Nature genetics》2008,40(12):1410-1412
Gerodermia osteodysplastica is an autosomal recessive disorder characterized by wrinkly skin and osteoporosis. Here we demonstrate that gerodermia osteodysplastica is caused by loss-of-function mutations in SCYL1BP1, which is highly expressed in skin and osteoblasts. The protein localizes to the Golgi apparatus and interacts with Rab6, identifying SCYL1BP1 as a golgin. These results associate abnormalities of the secretory pathway with age-related changes in connective tissues. 相似文献
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Tyler S. Smith Benjamin Spitzbarth Jian Li Donald R. Dugger Gabi Stern-Schneider Elisabeth Sehn Susan N. Bolch J. Hugh McDowell Jeremiah Tipton Uwe Wolfrum W. Clay Smith 《Cellular and molecular life sciences : CMLS》2013,70(23):4603-4616
Arrestins are dynamic proteins that move between cell compartments triggered by stimulation of G-protein-coupled receptors. Even more dynamically in vertebrate photoreceptors, arrestin1 (Arr1) moves between the inner and outer segments according to the light conditions. Previous studies have shown that the light-driven translocation of Arr1 in rod photoreceptors is initiated by rhodopsin through a phospholipase C/protein kinase C (PKC) signaling cascade. The purpose of this study is to identify the PKC substrate that regulates the translocation of Arr1. Mass spectrometry was used to identify the primary phosphorylated proteins in extracts prepared from PKC-stimulated mouse eye cups, confirming the finding with in vitro phosphorylation assays. Our results show that Bardet–Biedl syndrome 5 (BBS5) is the principal protein phosphorylated either by phorbol ester stimulation or by light stimulation of PKC. Via immunoprecipitation of BBS5 in rod outer segments, Arr1 was pulled down; phosphorylation of BBS5 reduced this co-precipitation of Arr1. Immunofluorescence and immunoelectron microscopy showed that BBS5 principally localizes along the axonemes of rods and cones, but also in photoreceptor inner segments, and synaptic regions. Our principal findings in this study are threefold. First, we demonstrate that BBS5 is post-translationally regulated by phosphorylation via PKC, an event that is triggered by light in photoreceptor cells. Second, we find a direct interaction between BBS5 and Arr1, an interaction that is modulated by phosphorylation of BBS5. Finally, we show that BBS5 is distributed along the photoreceptor axoneme, co-localizing with Arr1 in the dark. These findings suggest a role for BBS5 in regulating light-dependent translocation of Arr1 and a model describing its role in Arr1 translocation is proposed. 相似文献
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Plant aquaporin selectivity: where transport assays, computer simulations and physiology meet 总被引:3,自引:0,他引:3
Plants contain a large number of aquaporins with different selectivity. These channels generally conduct water, but some additionally
conduct NH3, CO2 and/or H2O2. The experimental evidence and molecular basis for the transport of a given solute, the validation with molecular dynamics
simulations and the physiological impact of the selectivity are reviewed here. The aromatic/arginine (ar/R) constriction is
most important for solute selection, but the exact pore requirements for efficient conduction of small solutes remain difficult
to predict. Yeast growth assays are valuable for screening substrate selectivity and are explicitly shown for hydrogen peroxide
and methylamine, a transport analog of ammonia. Independent assays need to address the relevance of different substrates for
each channel in its physiological context. This is emphasized by the fact that several plant NIP channels, which conduct several
solutes, are specifically involved in the transport of metalloids, such as silicic acid, arsenite, or boric acid in planta. 相似文献
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