Expression of recombinant human lysozyme in the milk of transgenic mice

Human lysozyme is a 130-aa (amino acid) alkaline polypeptide, and has both anti-bacterial and anti-viral properties which make it an important component of human natural immunity system. As a first step toward the ultimate goal of improving the anti-bacterial properties of bovine and ovine milk, a transgenic mouse that contains the genomic DNA sequence of the human lysozme gene has been generated for the first time. From 83 mice generated by microinjection, a total of 6 positive transgenic mice were identified by PCR and Southern blot. F1 mice positive for transgene in lines were also detected by PCR. This shows that transgene could be transmitted from founder transgenic mice to their offspring. Recombinant human lysozyme (rHlys) was found in the whey of 3 female positive transgenic mice by Western blot. The highest concentration of rHlys for transgenic mice was 0.2mg/mL. The antibacterial activity of the whey for transgenic mice was highly enhanced up to 0.4 times as much as that of human, while that of non-transgenic mouse was very low. Although the lysozyme activity of transgenic mice is still lower than that of human, the rHlys exhibits the same specific activity as that of human lysozyme. It provides a strong basis for further studies into the possible application of rHlys express in mammary gland.     

物流系统的合理化研究

指出物流系统合理性提出的原因,并论述了物流系统合理性的体现、作用及途径。     

浅析工程项目中的风险识别方法

文章在分析了项目工程风险的基础上，提出了工程项目风险辨识的一般方法，并对几种常用的方法进行了分析。     

城市固体垃圾热解设备与特性研究

针对无筛分城市垃圾热解的实用技术进行了研究,开发出内热源和外热源联合使用的垃圾热解设备.同时对设备的温度变化、垃圾热解特性、产气规律以及影响热解气热值的主要因素等进行了详细的分析.结果表明:实用热解设备的产气率高,理想热解温度在700℃以下.垃圾热解气中H2的体积分数达15.2%,C2H4,C2H6,C3H6和CH4的体积分数总和是10%,热解气的平均热值为7546.4 kJ/m3.设备运行稳定且温度特性满足设计要求.     

蒽甲醇修饰单壁碳纳米管的研究

利用蒽甲醇对单壁碳纳米管进行了化学修饰，并利用透射电镜、紫外可见吸收光谱、荧光光谱、拉曼光谱对修饰后的单壁碳纳米管进行了表征分析．通过对比吸附前后的紫外可见吸收光谱发现，吸附后的光谱强度下降大约57．1％，说明单壁碳纳米管吸附上了蒽甲醇．通过拉曼光谱分析发现，吸附后单壁碳纳米管的拉曼光谱中主要峰的位置向长波方向移动了6～7cm^-1，认为拉曼光谱发生移动的原因是单壁碳纳米管吸附蒽甲醇前后状态的改变导致的．     

Research on the User Interest Modeling of Personalized Search Engine

At present, how to enable Search Engine to construct user personal interest model initially, master user＇s personalized information timely and provide personalized services accurately have become the hotspot in the research of Search Engine area. Aiming at the problems of user model＇s construction and combining techniques of manual customization modeling and automatic analytical modeling, a User Interest Model （UIM） is proposed in the paper. On the basis of it, the corresponding establishment and update algorithms of User lnterest Profile （UIP） are presented subsequently. Simulation tests proved that the UIM proposed and corresponding algorithms could enhance the retrieval precision effectively and have superior adaptability.     

基于最小二乘法的自适应电流速断保护中系统参数的实时计算

对自适应电流速断保护中实时计算系统参数的算法进行了研究.提出了在系统发生故障时采用带补偿的全波傅氏算法,基于最小二乘法推导全波傅氏算法的补偿分量,并实时计算出系统的等值内电抗,然后再利用记忆在计算机存储器中的故障前的测量电压、电流采样值来计算系统等值内电势的相电势幅值Em.     

试论中国人法观念的文化继承

辛亥革命虽然结束了帝制时代,但传统法文化并没有消失,旧时代的法观念依然深深埋藏在中国人的内心深处。当代中国社会延续了人治时代对实质正义的追求,也继承了传统的人情、关系及恕道文化,导致对违法的宽容或认同,进而影响法治的进程。     

一种新型神经网络控制器的研究

提出了一种新型神经网络控制器,其控制机理与PID控制相类似,但解决了常规PID控制存在的快速性与超调量的矛盾.给出了该神经网络控制器的自适应学习算法,分析讨论了控制系统的稳定性.仿真实验表明,这种新型神经网络控制器构成的自适应控制系统具有较强的适应性和鲁棒性.     

新疆哈萨克大尾羊盲肠和结肠初袢综合肌电的活动规律

盲肠的电活动特征与结肠初袢的相同，均为以峰电活动为特征的电活动期与静息期交替出现的周期性电活动。盲肠、结肠初袢有长间隔（６３．２７±４．１３ｍｉｎ），短时程（５．１６±０．１３ｍｉｎ）的增强性峰电活动相，盲肠、结肠初袢的峰电具有移行性，常见的移行性峰电有５种类型，均与回肠无关系。