功能化石墨烯载hTERTsiRNA的制备及其对宫颈癌Hela细胞的影响

以氧化石墨烯(GrapheneOxide,GO)为基质,制备了氧化石墨烯-聚乙二醇-叶酸(GO-PEG-FA)纳米载体,借助于通过π-π相互作用负载在GO表面的胺甲基芘盐酸盐(PyNH2),介导人端粒酶反转录酶siRNA(hTERTsiRNA)转染入宫颈癌Hela细胞.GO-PEG-FA-PyNH2-hTERTsiRNA纳米基因复合物被成功制备,并通过红外光谱和紫外光谱进行表征.荧光显微镜观察该纳米载体的转染效率明显优于裸siRNA组,Western blot实验检测Hela细胞hTERT蛋白表达显著降低,MTT实验观察该载体对细胞无毒,其共载hTERTsiRNA和抗肿瘤药阿霉素后对Hela细胞生长的抑制具有协同作用.因而该功能化氧化石墨烯可以作为靶向基因载体,有效传递siRNA及化疗药物进入肿瘤细胞发挥作用.     

新型芴类发光共聚物

采用Suzuki偶合方法合成了萄与低带隙单体噻吩（Th)及其衍生物乙烯基二氧噻吩（EDT），4，7－二噻吩－2，1，3－苯并噻二唑（DBT），4，7－二噻吩－2，1，3－苯并硒二唑（BTSe)的二元无规共聚物，实现了聚芴颜色的调节，得到了发蓝绿光到黄光（峰值波长在490－560nm之间）以及饱和红光（峰值波长在628－718nm)的聚合物，所得到的共聚物的最大电致发光（EL）外量子效率为0．45％，芴与EDT共聚物的最大EL外量子效率为1．8％，芴与DBT共聚物的最大EL外量子效率为1．4％，均比文献中所报道的相应聚合物高得多，实验中还观察到了由于激子在低带隙单体DBT和BTSe位置的捕获而产生的有效的能量转移，这表明采用Suzuki偶合方法合成用少量低带隙单体掺杂至大带隙单体所得到的共聚物是一种极大发展前景的新型发光聚合物。     

Late Pleistocene glaciation of the Changbai Mountains in northeastern China

The Changbai Mountains （2749 m a.s.l.） in northeastern China are one of the typical mountain regions with glaciation since late Pleistocene as evidenced by well-preserved erosive and accumulative landforms at elevations above 2000 m a.s.l, formed by glaciers around the crater lake, Tianchi Lake. Cirque glaciers developed on both the inner and outer sides of the volcanic cone. Well-preserved cirques, glacial trough valleys, glacial threholds, polished surfaces of the glacial erratics and the moraine ridges indicate that several glaciation processes took place during the last glacial period in this region. Resuits of optically stimulated luminescence （OSL） dating on the moraine sediments, and the K/Ar, thermal ionization mass spectrometry （TIMS）, electronic spinning resonance （ESR） dating on the volcanic rocks suggest two periods of glacier advances. One is named the Black Wind Mouth glacier advance taking place on the west and north slopes of the volcanic cone at an elevation of 2000-2100 m a.s.l., which is dated to about 20 ka, being the result of the Last Glacial Maximum （LGM）. The other is named the Meteorological Station glacier advance at the elevation of 2400-2600 m a.s.l., dated to 11 ka during the late glacial period, and is tentatively correlated to the Younger Dryas stage. The scope of the former glacier advance is larger than that of the latter. Regional comparisons showed that the glacial sequences in the Changbai Mountains are similar to other glaciated areas in eastern Asia during the later part of the last glacial cycle.     

氯化锂-乙醇-水溶液稀释热

使用LKB 2107型流动式微量热计-微机数据采集系统测量了288.15 K、298.15 K和308.15 K下LiCl-C     

增量竞争的评定及方法

文章提出了经济发展中增量竞争的评定方式及其计算方法.     

桥式起重机运动模拟

在桥式起重机是应用最为广泛的起重机械，文章综合应用现代设计手段和方法，在桥架、起升机构、小车运行机构和大车运行机构的基础上，建立三维运动分析的数学模型，从而对整机和各部件的运动速度、加速度以及因运动引起的载荷进行分析，模拟各种运动规律和运动轨迹，从而实现起重机运行情况的模拟，为起重机的设计提供可靠的参考依据。     

引进ISO9001国际标准强化国外承包电站工程的质量管理

论述了引进ISO9001国际标准．健全质量保证体系，重视质量文件．强针质量监督与考核机制．从而提高企业在国际市场竞争力的做法与经验．     

The Textual Condition of Jane Austen's Sense and Sensibility

Literature has increasingly been seen as a text in a context. And text is a particular embodiment of the abstract idea that a work tries to exhibit. The textual condition of a publication involves not only the author but also the editor, publisher and readers. Text varies because of its ever-changing textual condition. Since its first publication in 1813, Jane Austen's novel Sense and Sensibility had been reproduced several times in the 19th century. The analysis of the novel's text and textuality in a way ...     

一种求闭环频率特性的图解法

本文介绍的求闭环频率特性的图解法，其特点是不用Ｎｉｃｈｏｌｓ图线或等Ｍ圆、等Ｎ圆图，而是通过向量叠加的办法，由开环频率特性求闭环频率特性。该方法计算量少，简单方便，适用于自动控制系统的分析与设计。     

苄基环戊二烯的相转移催化合成

碱性不介质中和四丁基溴化铵存在下，环戊二烯和氯化苄反应，合成了苄基环戊二烯。