重组肠激酶在甲醇酵母中高效表达的培养条件研究

对重组肠激酶在甲醇酵母中高效表达的发酵条件进行了优化，并在BiofloⅢ发酵罐中实现了高密度培养和高效表达。结果表明；温度30C，培养基pH6．0，当酵母密度达到200A600mm以上时，加入无水甲醇，控制甲醇的流加体积，使其终体积分数为1．0％～3．O％，继续诱导培养72h左右，酵母菌密度可达到150g／L，重组肠激酶总活性可达58kIU／L发酵液。     

Analysis of cDNA sequence, protein structure and expression of parotid secretory protein in pig

Parotid secretory protein (PSP) secreted abundantly in saliva, whose function is related with the anti-bacterial effect. The PSP cDNA has been isolated from pig parotid glands by 3′ and 5′ rapid amplification of cDNA end (RACE), based on the conserved signal peptide region among the known mammalian PSP. The result of homologous comparison shows that pig PSP and human PSP shares the high identity at the level of the primary, secondary and tertiary protein structure. A search for functionally significant protein motifs revealed a unique amino acid sequence pattern consisting of the residues Leu-X(6)-Leu-X(6)-Leu-X(7)-Leu-X(6)-Leu-X(6)-Leu near the amino-terminal portion of the protein, which is important to its function. RT-PCR, Dot blot and Northern blot analysis demonstrated that PSP was strongly expressed in parotid glands, but not in other tissues.     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

发动机用新型超导电磁减振器研究

针对传统减振器在性能上的固有缺陷，提出了用超导技术进行新型减振器设计，并对新型超导减振器的减振原理和产品结构进行了初步研究，探讨了新型超导减振器的控制方式，为进一步完善该产品性能提供了技术依据．     

BOT方式流行的原因及其风险分析

BOT方式作为发展大型基础设施的一种重要的融资与建造模式,近年来已成为国际建设工程承包领域最流行的建设方式之一。但实践表明风险问题是BOT模式的核心问题。本文对BOT模式流行的原因、BOT项目可能遇到的各种风险及相应的防范措施进行详尽的剖析。     

光纤传感技术监测混凝土大坝全相和时效分析方法

光纤监测混凝土大坝安全研究方法的探讨，是深入发展这一研究方向的重要课题。Q．S分析法、S．X分析法是通过鱼跳电站工程实践摸索的分析方法，它通过理论探讨和实践对照，为光纤技术应用于混凝土大坝安全监测积累了一定的经验。     

我国天然气资源的可持续发展与利用

通过结合世界及我国天然气事业的现状和发展前景，从经济、环境、资源、科技等方面协调发展的相互关系出发，提出了为保证我国天然气事业可持续发展，政府应在政策引导、建全法律法规、建立市场和合理补偿机制、发展西气东输等方面所应采取的措施．     

Turbo码译码算法的时延改进分析

通过对Turbo码最大似然译码MAP算法（maximum posteriori probability algorithm）和SOVA算法（soft output viterbi algorithm）的比较和对SOVA算法的比较深入的分析，对Turbo码在译码过程中存在的时延较大的问题做出了一些改进，并且针对引进时延的不同原因讨论了具体的解决方案，通过Matlab进行仿真，表明这些改进是有意义的。     

碳布补强含裂纹梁的断裂特性实验

为了解采用纤维复合材料加固含裂纹结构的断裂机理,通过光学焦散线方法对碳纤维布加固含裂纹梁进行3点弯荷载下的断裂实验,研究了其补强效果及断裂特性。实时记录了不同载荷状态下裂纹尖端应力集中引起的焦散斑光学图像,确定了不同初始裂纹长度的试件在粘贴加固不同长度碳布的情况下裂纹尖端的应力强度因子演化规律,分析了初始裂纹和粘贴碳布两种因素对加固效果的影响。实验结果表明:加固碳布可以大幅度地提高含裂纹梁强度,在该文实验中强度最大增加了近2倍。     

2-距离空间中几个不动点定理与Mann型迭代法

得到几个在2-距离空间中关于压缩型与扩张型的新的不动点定理，它扩充文[4]，[6]等中某些结果也得到关于两个映射的Mann型迭代法在Banach空间中的新结论．