Regulation of cell cycle progression and gene expression by H2A deubiquitination

Post-translational histone modifications have important regulatory roles in chromatin structure and function. One example of such modifications is histone ubiquitination, which occurs predominately on histone H2A and H2B. Although the recent identification of the ubiquitin ligase for histone H2A has revealed important roles for H2A ubiquitination in Hox gene silencing as well as in X-chromosome inactivation, the enzyme(s) involved in H2A deubiquitination and the function of H2A deubiquitination are not known. Here we report the identification and functional characterization of the major deubiquitinase for histone H2A, Ubp-M (also called USP16). Ubp-M prefers nucleosomal substrates in vitro, and specifically deubiquitinates histone H2A but not H2B in vitro and in vivo. Notably, knockdown of Ubp-M in HeLa cells results in slow cell growth rates owing to defects in the mitotic phase of the cell cycle. Further studies reveal that H2A deubiquitination by Ubp-M is a prerequisite for subsequent phosphorylation of Ser 10 of H3 and chromosome segregation when cells enter mitosis. Furthermore, we demonstrate that Ubp-M regulates Hox gene expression through H2A deubiquitination and that blocking the function of Ubp-M results in defective posterior development in Xenopus laevis. This study identifies the major deubiquitinase for histone H2A and demonstrates that H2A deubiquitination is critically involved in cell cycle progression and gene expression.     

大学图书馆创新发展之我见

随着信息技术的进步,大学图书馆必须实现管理与服务模式的转变,馆藏图书要随着学科、专业建设的发展而更新,使师生在教学、科研工作中接受最新的信息资源、接受到最新的社会动向,和社会保持同步前进。     

Fast computation of scattering from 3D complex structures by MLFMA

This paper introduces the research work on the extension of multilevel fast multipole algorithm （MLFMA） to 3D complex structures including coating object, thin dielectric sheet, composite dielectric and conductor, cavity. The impedance boundary condition is used for scattering from the object coated by thin lossy material. Instead of volume integral equation, surface integral equation is applied in case of thin dielectric sheet through resistive sheet boundary condition. To realize the fast computation of scattering from composite homogeneous dielectric and conductor, the surface integral equation based on equivalence principle is used. Compared with the traditional volume integral equation, the surface integral equation reduces greatly the number of unknowns. To computc conducting cavity with electrically large aperture, an electric field integral equation is applied. Some numerical results are given to demonstrate the validity and accuracy of the present methods.     

On the MIMO channel capacity saturation for spatially constrained receive region

In MIMO wireless communication systems,when more and more antennas are packed into spatiallylimited receive region,the antenna saturation phenomenon will appear.Moreover,the electromagnetic interactions among antennas will also become stronger and stronger and affect the antenna saturation effect considerably.Despite this,few studies consider these two effects jointly.The effects of antenna saturation are investigated under the consideration of mutual coupling,thus a more practical and physically meaningful result can be obtained.     

Surface nanostructures orienting self-protection of an orthodontic nickel-titanium shape memory alloys wire

Shape memory alloys (SMA) have been applied to a wide variety of applications in a number of different fields such as aeronautical applications, sensors/actuators, medical sciences as well as orthodontics. It is a hot topic to enhance the anti-corrosion ability of orthodontic wires for clinical applications. In this letter, a very nice fractal structure, micro-domains with identical nanometer sized grooves, was ob- tained on the surfaces of the orthodontic wires with an oxygen plasma and acid corrosion. The concave parts of the grooves were dominated by titanium and convex parts were the same as the bulk wires. The micro-nano fractal structure generated a hydrophobic surface with the largest contact angle to water being about 157°. The titanium dominated nanolayer and the hydrophobicity of the surface resulted in jointly the great improvement of the anti-corrosion ability of the orthodontic wires. Because the fractal structures of the wires were formed automatically when they immersed in acidic environment, hence, the self-protection of the oxygen plasma-treated orthodontic wires in acidic environment indicates their potential applications in orthodontics, and should be also inspirable for other applications of SMA materials.     

数字全息中直透光和共轭像的去除方法

去除直透光和共轭像的影响,提高全息再现像的质量,一直以来都是全息研究中的一个热点和难点,已有许多结合数字图像处理技术方法被提出.针对数字全息中直透光和共轭像的去除方法进行综述,说明各方法的优点、不足及其适用情况,并通过实验加以验证.     

一类p-Ginzburg-Landau型径向极小的零点分布和渐近性态

研究了一类与超导相关的p-Ginzburg-Landau 模型,其中p>2.给出了这一类泛函的径向极小元的零点分布,并证明这个极小元的W1,p局部收敛性.     

空袭目标威胁程度评估与排序

以军事理论和层次分析法为基础 ,将模糊优化理论及多目标最优化方法应用于空袭目标的威胁程度评估 ,为防空作战自动化指挥提供了一种辅助决策方法.     

芯片载体表面仿生膜的制备及探针固定研究

采用生物仿生的方式,利用多巴胺在玻片表面自聚合的特性,在玻片表面构建一层聚多巴胺薄膜。其自身含有的醌式结构,易于同带有活性官能团的生物分子发生化学反应而将生物分子固定到玻片表面。构建一种经济高效、绿色环保、操作简便的生物分子固定化平台,并与传统的固定方式进行对比。通过扫描电镜,红外光谱仪及荧光显微镜等对聚多巴胺薄膜进行表征。结果表明,制备薄膜由15~20nm的纳米颗粒构成,因此制备薄膜具有更高的比表面积及更小的空间位阻,利于提高探针的固定量及效率。通过荧光显微镜成像及Image J软件统计分析,与传统的制备方法比较,该固定方法能够提高固定探针的数量及荧光强度,因而该方法优于传统的固定方法,在生物芯片载体表面修饰方面具有广阔的应用前景。     

基于虚拟现实的船舶操纵训练系统相关技术

介绍了船舶操纵训练系统的构成 ,该系统使用多通道视频拼接的方法生成 360°视景 .提出了视景中海浪的建模方法 ,包括静止海浪模型和动态海浪模型 .通过采用局域网环境下的并行处理、增量发送、消息包定长定构和事件驱动接收等计算机网络与通信技术实现了具有较高的性能价格比的船舶操纵训练器 .还给出了白天航行、夜航、雾航和大风浪航行等环境条件下的仿真结果