基于五片DSPs的JPEG2000压缩系统

由于JPEG2000的离散小波变换(DWT)的提升算法和嵌入块编码(EBCOT)占85%以上的处理时间,因此,提出一种基于TMS320C6713 DSPs的1 392×1 040像素、40帧/s的JPEG2000压缩系统的设计方案.它采用并行编码处理的五片DSPs结构系统、改进9/7小波变换算法、运用高效的内存管理和EBCOT编码的并行处理.实验结果表明本方案在DWT执行速度上是现有方案的2倍多.     

NaCl胁迫下枸杞愈伤组织可溶性蛋白含量的变化

以枸杞愈伤组织为材料,分别接种于对照及含100,200,400mmol／L NaCl的培养基上,培养1,3,5,7,11d．实验结果表明,随着盐浓度的升高．枸杞愈伤组织可溶性蛋白含量降低,且随着时间的延长呈先上升后下降的趋势,即随着盐浓度的升高,培养1,3,5d时可溶性蛋白含量上升,5d后开始下降;培养11d时在不同含盐培养基上加入外源ABA,愈伤组织可溶性蛋白含量升高,通过SDS-PAGE电泳实验发现,当NaCl浓度为400mmol／L时,在愈伤组织中诱导出一条40．5kD的新蛋白．     

Probing cellular protein complexes using single-molecule pull-down

Proteins perform most cellular functions in macromolecular complexes. The same protein often participates in different complexes to exhibit diverse functionality. Current ensemble approaches of identifying cellular protein interactions cannot reveal physiological permutations of these interactions. Here we describe a single-molecule pull-down (SiMPull) assay that combines the principles of a conventional pull-down assay with single-molecule fluorescence microscopy and enables direct visualization of individual cellular protein complexes. SiMPull can reveal how many proteins and of which kinds are present in the in vivo complex, as we show using protein kinase A. We then demonstrate a wide applicability to various signalling proteins found in the cytosol, membrane and cellular organelles, and to endogenous protein complexes from animal tissue extracts. The pulled-down proteins are functional and are used, without further processing, for single-molecule biochemical studies. SiMPull should provide a rapid, sensitive and robust platform for analysing protein assemblies in biological pathways.     

Development of a propulsion system for a biomimetic thruster

We studied theoretically and experimentally a biomimetic propulsion system inspired by the motility mechanisms of bacteria such as E. coli. Our goal was to investigate the effect of the ??complex?? filament of Rhizobium Meliloti bacteria on thrust force. The complex filament is a helically perturbed filament, similar to a plain filament threaded through a small helix. The propulsive performance of this system was estimated by modeling the dynamics of helical wave motion in viscous fluid. The model consists of a helical filament which is axially rotated at angular velocity ??. Resistive force theory (RFT) was applied to this model to calculate the thrust force and required torque. The Buckingham PI theorem (non-dimensional analysis) was also used to analyze the theoretical results. The procedure for making a complex filament with various pitch angles ?? _s from a small helix and plain filament is explained in detail. To validate the theoretical results for helical wave propulsion and compare the characteristics of complex and plain filaments together, an experiment was performed to measure the thrust forces in silicone oil. The experimental results agreed with the theoretical values predicted by RFT. The thrust forces of complex filaments depended on the shape of small helix winding. The maximum thrust force was achieved at a small helix pitch angle of ?? _s = 45°. In addition, we found that the thrust force generated by a complex filament had a value about 10% higher than that of a plain filament with the same equivalent diameter d _e .     

Research on Container Behaviors Protocol

With the development of middleware technology, there are some new features to be adopted on demand in middleware platform based on container component. Aiming at the adaptation of middleware platform, from the nature of component container, this paper begins with behaviors features of EJB （Enterprise Java Bean） container, extracts general behaviors protocol of container, and defines its formal behaviors specification. In the end, the interactive relationship of components is described by WRIGHT language, and the configuration of whole system is shown. The method proposed in this paper mines the hidden features of framework of component container, and applies to refactor middleware platform in reflection and aspect-oriented programming technology.     

Features of tropical cyclone landfalls over East Asia corresponding to three types of Pacific warming decaying phase

This study examines the tropical cyclone （TC） landfall activities over the East Asia in three types of decaying phase of warm sea surface temperature anomalies （SSTAs） over the equatorial central-eastern Pacific： eastern Pacific warming decaying to La Nifia, eastern Pacific warming decaying to a neutral E1 Nifio-Southern Oscilla- tion phase, and a central Pacific warming decaying year. Results show that, for the type of eastern Pacific wanning decaying to La Nifia, more TCs make landfall over Hainan Island and Beibu Gulf, whereas fewer TCs reach eastern China coast. In particular, the number of landfalling TCs remarkably decreases in the decaying phase of eastern Pacific E1 Nifio to a neutral year. During the decaying phase of central Pacific E1 Nifio events, more TCs tend to make landfall over southern China, Indochina Peninsula and the Philippines. The anomalies of atmospheric circu- lation and environmental conditions induced by the SSTAs over the tropical Pacific in the different decaying types are responsible for the evident variation in features of TC landfall.     

基于动测法的基桩刚度验证试验研究

针对以往基桩刚度无法准确测得的情况,从基桩动力特性基本理论出发,重新梳理了桩顶受微小激励时所产生的动态响应,给出了通过速度导纳幅频曲线中振动固有频率的位置来计算基桩刚度值的基本公式。利用室内模型试验,通过静态加载法和瞬态机械阻抗法对比测定基桩刚度值,验证了瞬态机械阻抗法测试基桩刚度值的可行性。该结果进一步说明了通过瞬态机械阻抗速度幅频曲线拾取基桩振动固有频率来确定基桩刚度值是具有实际意义的。     

Compartmentalized calcium dynamics in a C. elegans interneuron encode head movement

The confinement of neuronal activity to specific subcellular regions is a mechanism for expanding the computational properties of neurons. Although the circuit organization underlying compartmentalized activity has been studied in several systems, its cellular basis is still unknown. Here we characterize compartmentalized activity in Caenorhabditis elegans RIA interneurons, which have multiple reciprocal connections to head motor neurons and receive input from sensory pathways. We show that RIA spatially encodes head movement on a subcellular scale through axonal compartmentalization. This subcellular axonal activity is dependent on acetylcholine release from head motor neurons and is simultaneously present and additive with glutamate-dependent globally synchronized activity evoked by sensory inputs. Postsynaptically, the muscarinic acetylcholine receptor GAR-3 acts in RIA to compartmentalize axonal activity through the mobilization of intracellular calcium stores. The compartmentalized activity functions independently of the synchronized activity to modulate locomotory behaviour.     

Autistic-like social behaviour in Shank2-mutant mice improved by restoring NMDA receptor function

Autism spectrum disorder (ASD) is a group of conditions characterized by impaired social interaction and communication, and restricted and repetitive behaviours. ASD is a highly heritable disorder involving various genetic determinants. Shank2 (also known as ProSAP1) is a multi-domain scaffolding protein and signalling adaptor enriched at excitatory neuronal synapses, and mutations in the human SHANK2 gene have recently been associated with ASD and intellectual disability. Although ASD-associated genes are being increasingly identified and studied using various approaches, including mouse genetics, further efforts are required to delineate important causal mechanisms with the potential for therapeutic application. Here we show that Shank2-mutant (Shank2(-/-)) mice carrying a mutation identical to the ASD-associated microdeletion in the human SHANK2 gene exhibit ASD-like behaviours including reduced social interaction, reduced social communication by ultrasonic vocalizations, and repetitive jumping. These mice show a marked decrease in NMDA (N-methyl-D-aspartate) glutamate receptor (NMDAR) function. Direct stimulation of NMDARs with D-cycloserine, a partial agonist of NMDARs, normalizes NMDAR function and improves social interaction in Shank2(-/-) mice. Furthermore, treatment of Shank2(-/-) mice with a positive allosteric modulator of metabotropic glutamate receptor 5 (mGluR5), which enhances NMDAR function via mGluR5 activation, also normalizes NMDAR function and markedly enhances social interaction. These results suggest that reduced NMDAR function may contribute to the development of ASD-like phenotypes in Shank2(-/-) mice, and mGluR modulation of NMDARs offers a potential strategy to treat ASD.